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Category Archives: Cell Therapy
Kansas RMC Stem Cell Therapy in the US – Video
Posted: September 1, 2014 at 5:43 am
Kansas RMC Stem Cell Therapy in the US
In the past, stem cell treatment was only available in Europe. Now, in the US, we are able to provide this potentially life changing treatment to people like you at a very reasonable price....
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Kansas RMC Stem Cell Therapy in the US - Video
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Stem Cell Activation Phuket, Thailand: How is your stem cell therapy different – Video
Posted: August 30, 2014 at 5:48 am
Stem Cell Activation Phuket, Thailand: How is your stem cell therapy different
http://www.thanyapurahealth.com/health-services/natural-stem-cell-activationregenerative-therapy/how-is-your-stem-cell-therapy-different/ Thanyapura Health offers natural stem cell activation,...
By: Thanyapura Health Centre
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Stem Cell Activation Phuket, Thailand: How is your stem cell therapy different - Video
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Stem Cell Activation Phuket, Thailand: How have the stem cell therapy results been so far – Video
Posted: August 30, 2014 at 5:48 am
Stem Cell Activation Phuket, Thailand: How have the stem cell therapy results been so far
http://www.thanyapurahealth.com/health-services/natural-stem-cell-activationregenerative-therapy/stem-cell-activation-phuket-thailand-how-have-the-stem-cell-therapy-results-been-so-far/ Client...
By: Thanyapura Health Centre
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Stem Cell Activation Phuket, Thailand: How have the stem cell therapy results been so far - Video
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Stem Cell Activation Phuket: What type of clients have you been seeing for stem cell therapy – Video
Posted: August 30, 2014 at 5:48 am
Stem Cell Activation Phuket: What type of clients have you been seeing for stem cell therapy
http://www.thanyapurahealth.com/health-services/natural-stem-cell-activationregenerative-therapy/what-type-of-clients-have-you-been-seeing-for-stem-cell-therapy/ Wide range of client who has...
By: Thanyapura Health Centre
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Stem Cell Activation Phuket: What type of clients have you been seeing for stem cell therapy - Video
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Stem Cell Activation Phuket, Thailand: How have athletes been using stem cell therapy – Video
Posted: August 30, 2014 at 5:48 am
Stem Cell Activation Phuket, Thailand: How have athletes been using stem cell therapy
http://www.thanyapurahealth.com/health-services/natural-stem-cell-activationregenerative-therapy/how-have-athletes-been-using-stem-cell-therapy/ Using stem cell therapy treats body issue e.g....
By: Thanyapura Health Centre
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Stem Cell Activation Phuket, Thailand: How have athletes been using stem cell therapy - Video
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The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical …
Posted: August 30, 2014 at 5:48 am
Boston, MA (PRWEB) August 29, 2014
Due to a misstatement posted by the Family Resource Council (FRC) last week, the Adult Stem Cell Technology Center, LLC (ASCTC) was reported as an entity accepting donations to support stem cell therapy research that might benefit ALS patients. Because of ASCTC's exclusive focus on developing adult stem cells for new stem cell technologies and therapeutic applications, FRC listed ASCTC as one of several alternatives for ALS Ice Bucket Challenge donors who wish not to support research that requires the death of human embryos or fetal abortions.
After authorizing the listing by FRC, ASCTC's Director James Sherley states that he was surprised by suddenly receiving a number of communications from individuals inquiring how to donate to ASCTC. A look at the FRC posting explained the contacts. Inadvertently, ASCTC had been indicated as an agency that received donations for stem cell research. After ASCTC notified FRC staff of the mistake, the posting was immediately corrected. Since the correction, Director Sherley reports that the number of donation contacts has decreased, but not stopped completely.
In addition to the FRC correction, the ASCTC now responds to donation inquiries with the following statement:
Thank you for your interest in donating to ASCTC to support its development efforts in adult stem cell technologies. If your query was motivated by a recent Family Resource Council (FRC) notice, unfortunately, the notice posted by FRC was mistaken in its 'donate' message and did not adequately indicate that ASCTC is a for-profit company. Therefore, there is no means by which to 'donate' to the company. At my request, FRC has now corrected the posting. It is possible to invest in ASCTC commercial development of technologies for producing and quantifying human adult stem cells for drug development and therapeutics. However, this was not the intent of the FRC notice. Its purpose was to make the public aware of all the entities, not-for-profit or for-profit, engaged in developing stem cell technologies, potentially relevant to finding a cure for ALS, that do not use human embryonic stem cells. Please, accept my apologies for the miscommunication; and thank you for your good will in supporting ethical research to help others suffering from devastating illnesses like ALS.
Sincerely,
James L. Sherley Director ASCTC, LLC
Director Sherley observed, The best thing about this unfortunate situation is that I have had the pleasure of communicating with some very caring peopleand that is quite inspiring! ************************************************************************************************************* The Adult Stem Cell Technology Center, LLC (ASCTC) is a Massachusetts life sciences company established in September 2013. ASCTC Director and founder, James L. Sherley, M.D., Ph.D. is the foremost authority on the unique properties of adult stem cells. The companys patent portfolio contains biotechnologies that solve the two main technical problems production and quantification that have stood in the way of successful commercialization of human adult tissue stem cells for regenerative medicine and drug development. In addition, the portfolio includes novel technologies for isolating cancer stem cells and producing iPSCs. Currently, ASCTC is employing its technological advantages to pursue commercialization of mass-produced therapeutic human liver cells and facile assays that are early warning systems for drug candidates with catastrophic toxicity due to adverse effects against adult tissue stem cells.
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The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical ...
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New Reprogramming Factor Cocktail Produces Therapy-Grade Induced Pluripotent Stem Cells
Posted: August 29, 2014 at 12:45 am
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Newswise CAMBRIDGE, Mass. (September 4, 2014) Induced pluripotent stem cells (iPSCs)adult cells reprogrammed back to an embryonic stem cell-like statemay hold the potential to cure damaged nerves, regrow limbs and organs, and perfectly model a patients particular disease. Yet through the reprogramming process, these cells can acquire serious genetic and epigenetic abnormalities that lower the cells quality and limit their therapeutic usefulness.
When the generation of iPSCs was first reported in 2006, efficiency was paramount because only a fraction of a percentage of reprogrammed cells successfully became cell lines. Accordingly, the stem cell field focused on reprogramming efficiency to boost the pool of cells that could be studied. However, as scientists gained an increased understanding of the reprogramming process, they realized that myriad variables, including the ratio of reprogramming factors and the reprogramming environment, can also greatly affect cell quality.
Now researchers working in the lab of Whitehead Institute Founding Member Rudolf Jaenisch together with scientists from the Hebrew University have determined that the reprogramming factors themselves impact the reprogramming efficiency and the quality of the resulting cells. Their work is described in the current issue of the journal Cell Stem Cell.
Postdoctoral researcher Yosef Buganim and Research Scientist Styliani Markoulaki show that a different combination of reprogramming factors may be less efficient than the original, but can produce much higher quality iPSCs, says Jaenisch, who is also a professor of biology at MIT. And quality is a really important issue. At this point, it doesnt matter if we get one colony out of 10,000 or one out of 100,000 cells, as long as it is of high quality.
To make iPSCs, scientists expose adult cells to a cocktail of genes that are active in embryonic stem cells. iPSCs can then be pushed to differentiate into almost any other cell type, such as nerve, liver, or muscle cells. Although the original combination of Oct4, Sox2, Klf4, and Myc (OSKM) efficiently reprograms cells, a relatively high percentage of the resulting cells have serious genomic aberrations, including aneuploidy, and trisomy 8, which make them unsuitable for use in clinical research.
Using bioinformatic analysis of a network of 48 genes key to the reprogramming process, Buganim and Markoulaki designed a new combination of genes, Sall4, Nanog, Esrrb, and Lin28 (SNEL). Roughly 80% of SNEL colonies made from mouse cells were of high quality and passed the most stringent pluripotency test currently available, the tetraploid complementation assay. By comparison, only 20-30% of high quality OSKM passed the same test. Buganim hypothesizes that SNEL reprograms cells better because, unlike OSKM, the cocktail does not rely on a potent oncogene like Myc, which may be causing some of the genetic problems. More importantly, the cocktail does not rely on the potent key master regulators Oct4 and Sox2 that might abnormally activate some regions in the adult cell genome.
To better understand why some reprogrammed cells are of high quality while others fall short, Buganim and Markoulaki analyzed SNEL colonies down to the genetic and epigenetic level. On their DNA, SNEL cells have deposits of the histone protein H2AX in locations very similar to those in ESCs, and the position of H2AX seems to predict the quality of the cell. The researchers believe this characteristic could be used to quickly screen for high quality colonies.
But for all of its promise, the current version of SNEL seems unable to reprogram human cells, which are generally more difficult to manipulate than mouse cells.
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New Reprogramming Factor Cocktail Produces Therapy-Grade Induced Pluripotent Stem Cells
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STEM CELL THERAPY: with DR ANDREW J. ROCHMAN – Video
Posted: August 28, 2014 at 5:47 am
STEM CELL THERAPY: with DR ANDREW J. ROCHMAN
Dr. Andrew J Rochman is a leader in advanced surgical techniques, a native New Yorker and a Board-Certified Surgeon. His current undertaking involves promoting an educational advocacy in MODERN...
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STEM CELL THERAPY: with DR ANDREW J. ROCHMAN - Video
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Hip arthroscopy and stem cell therapy – a patients view – Video
Posted: August 27, 2014 at 6:45 am
Hip arthroscopy and stem cell therapy - a patients view
Anne is a personal trainer from Oslo Norway, she is extremely active lady involved in lots of outdoor sports including Nordic walking, skiing, running, marti...
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Hip arthroscopy and stem cell therapy - a patients view - Video
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Method developed to print replacement tissues using stem cells
Posted: August 27, 2014 at 6:45 am
Prof Frank Barry, scientific director of the Regenerative Medicine Institute at NUI Galway, with PhD student Babu Rajendra Prasad. Photograph: Joe OShaughnessy
By using tiny cartridges dispensing one stem cell at a time, Galway-based researchers may soon be able to literally print the scaffold of a healthy human tissue, and let it grow to become a therapeutic transplant.
When the Regenerative Medicine Institute at NUI Galway and Irish start-up company Poly-Pico Ltd recently joined forces for a trial proof-of-concept experiment, the results were spectacular.
They were able to dispense tiny drops from a cartridge filled with a stem cell mixture, each drop containing no more than a single stem cell.
Now imagine that we have five dispensing cartridges, each containing a different type of programmed stem cell, said Frank Barry, professor of cellular therapy and scientific director of the institute.
In principle we could essentially print them on to a surface and, by repeating the process a few thousands of times, obtain a mixture of growing cells and eventually a healthy pancreatic islet.
The islets produced by the printing process would then be transplanted into the pancreas of a Type 1 diabetic patient. The hope is that they will develop there and eventually help with the regulation of blood sugar levels.
It is a futuristic prospect, but it is not science fiction, Prof Barry said.
We are talking five years down the line for potential clinical trials.
In the experiment, the drops containing a single stem cell were easily identified and isolated. The cells were then allowed to replicate themselves into exact copies. Finally the researchers checked that they had remained viable and unaffected by the process.
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Method developed to print replacement tissues using stem cells
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