Category Archives: Stem Cells

Scientists from Queen Mary University of London have shown that stem cell behaviour can be modified by manipulating the nanoscale properties of the material they are grown on - improving the potential of regenerative medicine and tissue engineering as a result.

Stem cells are special because they are essential to the normal function of our organs and tissues. Previous research shows stem cells grown on hard substrates go on to multiply but do not differentiate: a process by which the cells specialise to perform specific functions in the body. In contrast, stem cells grown on softer surfaces do go on to differentiate.

In this new study, published in the journal Nano Letters, the researchers used tiny material patches known as nanopatches to alter the surface of the substrate and mimic the properties of a softer material.

"By changing the surface properties like the shape of the substrate at the nanoscale level, we tricked the stem cells to behave differently," explains co-author Dr Julien Gautrot, from QMUL's School of Engineering and Materials Science and the Institute of Bioengineering.

The team tested different sizes of nanopatches - from 3 microns to 100 nanometres (about one thousandth of the diameter of a hair). The stem cells behaved as if they were on a soft surface when in contact with the smallest patches because they can't firmly grip them.

Dr Gautrot added: "This development will be useful when there's a need to create a rigid implant to be inserted into the body. Potentially, such nanopatches could provide a soft touch to the surface of the implant so that cells from the neighbouring tissues are not perturbed by such a hard material."

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Stem cells are a soft touch for nano-engineered biomaterials

NBC News -- Researchers have grown part of an eye in a lab dish, using a type of stem cell made from a piece of skin.

They said the little retina started growing and developing on its own an important step towards creating custom-tailored organs in the lab.

We have basically created a miniature human retina in a dish that not only has the architectural organization of the retina but also has the ability to sense light," said M. Valeria Canto-Soler, an assistant professor of ophthalmology at the Johns Hopkins University School of Medicine.

The team used cells called induced pluripotent stem cells, or iPS cells, which are immature stem cells whose powers resemble those of embryonic stem cells they can morph into any cell type in the body.

Theyre made by tricking an ordinary cell, like a skin cell, into reverting back into embryonic mode. Then the researchers activate genes to get the cell to redirect itself into forming the desired cells in this case cells of the retina.

To the surprise of the researchers, the cells started developing as if they were in a growing human embryo.

"We knew that a 3-D cellular structure was necessary if we wanted to reproduce functional characteristics of the retina, but when we began this work, we didn't think stem cells would be able to build up a retina almost on their own. In our system, somehow the cells knew what to do, Canto-Soler said in a statement.

The experiment may ultimately lead to technologies that restore vision in people with retinal diseases, she added.

Tests showed the cells responded to light, the team reported in the journal Nature Communications. "Is our lab retina capable of producing a visual signal that the brain can interpret into an image? Probably not, but this is a good start," Canto-Soler said.

Other teams have used iPS cells to make a piece of human liver and are using them to study a range of human diseases.

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Eye in a Dish: Researchers Make Retina From Stem Cells

Mice severely disabled by a condition similar to multiple sclerosis (MS) could walk less than two weeks following treatment with human stem cells. The study, which uncovers new avenues for treating MS, was don e at the University of Utah and published online on May 15th 2014, in the journal Stem Cell Reports.

The scientists we surprised and encouraged by their finding. When they transplanted human stem cells into MS mice, they expected no benefit from the treatment. They thought the cells would be rejected, much like rejection of an organ transplant. Instead, the experiment yielded spectacular results.

A release from the university quotes co-senior author, Tom Lane, Ph.D., a professor of pathology at the University of Utah, who began the study at the University of California, Irvine, as saying, My postdoctoral fellow Dr. Lu Chen came to me and said, The mice are walking. I didnt believe her.

Within a short period of time, 10 to 14 days, the mice could walk and run. Six months later, they showed no signs of slowing down.

This result opens up a whole new area of research for us to figure out why it worked, said co-senior author Jeanne Loring, Ph.D., director of the Center for Regenerative Medicine at The Scripps Research Institute in La Jolla, California.

More than 2.3 million people worldwide have MS, a disease in which the immune system attacks myelin, an insulation layer surrounding nerve fibers. The resulting damage inhibits transmission of nerve impulses, producing a wide array of symptoms including difficulty walking, impaired vision, fatigue and pain.

Current FDA-approved medications slow early forms of the disease by dampening attacks by the immune system. In recent years, scientists have turned their attention to searching for ways to halt or reverse MS. Such a discovery could help patients with latter, or progressive, stages of the disease, for whom there are no treatments.

Results from the study demonstrate the mice experience at least a partial reversal of symptoms. Immune attacks are blunted, and the damaged myelin is repaired, explaining their dramatic recovery.

The way we made the neural stem cells turns out to be important, said Loring, describing the reason behind the novel outcome.

Prior to transplantation, Lorings graduate student and co-first author on the paper, Ronald Coleman, followed his intuition and grew the cells so they were less crowded on the Petri dish than usual. The change in protocol yielded a human neural stem cell type that turned out to be extremely potent. The experiments have since been successfully repeated with cells produced under the same conditions, but by different laboratories.

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Mice With MS Walk Again After Stem Cell Tx

Ravi Jagasia - Modeling ASD using human pluripotent stem cells
Ravi Jagasia, Human Neural Stem Cell Lab, Roche, IMI EU-AIMS project.

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Ravi Jagasia - Modeling ASD using human pluripotent stem cells - Video

University of Waikato Biological Sciences student Sarah Appleby has won a C. Alma Baker Postgraduate Scholarship for her agriculture-focused masters research project.

Bovine embryonic stem cell research

The Massey University scholarship, worth $13,000, will go towards Sarahs research into bovine embryonic stem cells.

"Im working with Dr Bjorn Oback at AgResearch on bovine embryonic stem cells and testing their functional potential. Embryonic stem cells have really only been fully isolated from mice and rats, however the group at AgResearch (Ruakura) have developed a method that isolates cells from cows that have very similar properties to the mice embryonic stem cells. Tests have already shown that the cells perform well in the lab, so my work will be focused on seeing if they improve cloning efficiency," says Sarah.

"The aim of this work is to improve our understanding of bovine embryonic stem cells for their use in accelerated animal breeding and biomedical applications. If we can effectively capture the best traits using embryonic stem cells we could develop better biotechnology-based breeding systems to have animals that are more efficient and sustainable to farm."

Hands-on work placements

The former Whangamata Area School student completed a Bachelor of Science (Technology) at Waikato University and is half way through a Master of Science majoring in Biological Sciences, with an emphasis on physiology and genetics.

This is not the first time that Sarah has worked with Dr Oback. During a BSc(Tech) work placement, she spent three months at AgResearch (Ruakura), on a bovine karyotyping project. During another placement, Sarah also spent seven months at AgResearch (Invermay), working under Dr Jenny Juengel on a number of projects related to sheep fertility and reproduction.

"I absolutely loved being part of those teams and getting some hands on lab experience, especially in the IVF field."

Sarah heard about the BSc(Tech) degree while at school. "Nowhere else offered a degree like it, so I chose to come to Waikato. I really liked the idea of the placements, which sounded like the perfect way to get real world experience while completing my degree."

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Scholarship to support student's embryonic stem cell research

Human embryonic stem cells the bodys powerful master cells might be useful for treating multiple sclerosis, researchers reported Thursday.

A team has used cells taken from frozen human embryos and transformed them into a type of cell that scientists have hoped might help treat patients with MS, a debilitating nerve disease.

Mice with an induced version of MS that paralyzed them were able to walk freely after the treatment, the teams at Advanced Cell Technology and ImStem Biotechnology in Farmington, Connecticut, reported.

The cells appeared to travel to the damaged tissues in the mice, toning down the mistaken immune system response that strips the fatty protective layer off of nerve calls. Its that damage that causes symptoms ranging from tremors and loss of balance to blurry vision and paralysis.

These embryonic stem cells were carefully nurtured to make them form a type of immature cell called a mesenchymal stem cell. These cells worked better to treat the mice than naturally developed mesenchymal stem cells taken directly from bone marrow, the team wrote in the journal Stem Cell Reports, published by the International Society for Stem Cell Research.

The top mouse is paralyzed, while the mouse on the bottom was treated with human embryonic stem cells and is able to run around.

The company released a video to show the benefits. Untreated mice were suffering. They are paralyzed. They on their backs. They are dragging their limbs. They are in really sad shape, ACTs chief scientific officer, Dr. Bob Lanza, told NBC News.

Treated animals, they are walking and jumping around just like normal mice.

Lanza says human trials are many months away, but he thinks it will not be necessary to use controversial cloning technology to make perfectly matched human embryonic stem cells to treat patients.

We can use an off-the-shelf source and itll work for everyone, he said. So you can use them and not worry about rejection.

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Stem cells work on MS in mice

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New York, June 7 : Liver is known to regenerate itself, but can liver cells be reverted to a stem-cell like state? Yes, researchers say.

The finding can pave the way for liver cells transplant to cure genetic liver disorders.

Researchers at the Harvard Stem Cell Institute, led by Fernando Camargo, stumbled upon this fact while investigating whether a biochemical cascade called "Hippo", which controls how big the liver grows, also affects the cell's fate.

"I think this study highlights the tremendous plasticity of mature liver cells," said Camargo, an associate professor at Harvard University's department of stem cell and regenerative biology.

The researchers found that switching off the Hippo-signalling pathway in mature liver cells generates very high rates of dedifferentiation.

This means the cells turn back the clock to become stem-cell like again, thus allowing them to give rise to functional progenitor cells that can regenerate a diseased liver.

Even if three-quarters of a liver is surgically removed, duplication alone could return the organ to its normal functioning mass.

This new research indicates that there is a second mode of regeneration that may be repairing constant liver damage.

"It is not that you have a very small population of cells that can be recruited to an injury. Almost 80 percent of liver cells can undergo this cell fate change," Camargo added.

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Mature liver cells can turn into stem cells!

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Newswise When stem cells are used to regenerate bone tissue, many wind up migrating away from the repair site, which disrupts the healing process. But a technique employed by a University of Rochester research team keeps the stem cells in place, resulting in faster and better tissue regeneration. The key, as explained in a paper published in Acta Biomaterialia, is encasing the stem cells in polymers that attract water and disappear when their work is done.

The technique is similar to what has already been used to repair other types of tissue, including cartilage, but had never been tried on bone.

Our success opens the door for manyand more complicatedtypes of bone repair, said Assistant Professor of Biomedical Engineering Danielle Benoit. For example, we should now be able to pinpoint repairs within the periosteumor outer membrane of bone material.

The polymers used by Benoit and her teams are called hydrogels because they hold water, which is necessary to keep the stem cells alive. The hydrogels, which mimic the natural tissues of the body, are specially designed to have an additional feature thats vital to the repair process; they degrade and disappear before the body interprets them as foreign bodies and begins a defense response that could compromise the healing process.

Because stem cells have the unique ability to develop into many different types of cells, they are an important part of the mechanism for repairing body tissue. At present, unadulterated therapeutic stem cells are injected into the bone tissue that needs to be repaired. Benoit believed hydrogels would allow the stem cells to finish the job of initiating repairs, then leave before overstaying their welcome.

The research team tested the hypothesis by transplanting cells onto the surface of mouse bone grafts and studying the cell behavior both in vivoinside the animaland in vitrooutside the body. They started by removing all living cells from donor bone fragments, so that the tissue regeneration could be accomplished only by the stem cells.

In order to track the progress of the research, the stem cells were genetically modified to include genes that give off fluorescence signals. The bone material was then coated with the hydrogels, which contained the fluorescently labeled stem cells, and implanted into the defect of the damaged mouse bone. At that point, the researchers began monitoring the repair process with longitudinal fluorescence to determine if there would be an appreciable loss of stem cells in the in vivo samples, as compared to the static, in vitro, environments. They found that there was no measurable difference between the concentrations of stem cells in the various samples, despite the fact that the in vivo sample was part of a dynamic environmentwhich included enzymes and blood flowmaking it easier for the stem cells to migrate away from the target site. That means virtually all the stem cells stayed in place to complete their work in generating new bone tissue.

Some types of tissue repair take more time to heal than do others, said Benoit. What we needed was a way to control how long the hydrogels remained at the site.

See the article here:
Better Tissue Healing with Disappearing Hydrogels

Human embryonic stem cells the bodys powerful master cells might be useful for treating multiple sclerosis, researchers reported Thursday.

A team has used cells taken from frozen human embryos and transformed them into a type of cell that scientists have hoped might help treat patients with MS, a debilitating nerve disease.

Mice with an induced version of MS that paralyzed them were able to walk freely after the treatment, the teams at Advanced Cell Technology and ImStem Biotechnology in Farmington, Connecticut, reported.

The cells appeared to travel to the damaged tissues in the mice, toning down the mistaken immune system response that strips the fatty protective layer off of nerve calls. Its that damage that causes symptoms ranging from tremors and loss of balance to blurry vision and paralysis.

These embryonic stem cells were carefully nurtured to make them form a type of immature cell called a mesenchymal stem cell. These cells worked better to treat the mice than naturally developed mesenchymal stem cells taken directly from bone marrow, the team wrote in the journal Stem Cell Reports, published by the International Society for Stem Cell Research.

The top mouse is paralyzed, while the mouse on the bottom was treated with human embryonic stem cells and is able to run around.

The company released a video to show the benefits. Untreated mice were suffering. They are paralyzed. They on their backs. They are dragging their limbs. They are in really sad shape, ACTs chief scientific officer, Dr. Bob Lanza, told NBC News.

Treated animals, they are walking and jumping around just like normal mice.

Lanza says human trials are many months away, but he thinks it will not be necessary to use controversial cloning technology to make perfectly matched human embryonic stem cells to treat patients.

We can use an off-the-shelf source and itll work for everyone, he said. So you can use them and not worry about rejection.

Read this article:
Stem Cells Treat Multiple Sclerosis in Mice

PUBLIC RELEASE DATE:

5-Jun-2014

Contact: Robert Miranda cogcomm@aol.com Cell Transplantation Center of Excellence for Aging and Brain Repair

Putnam Valley, NY. (June 5, 2014) Scientists in Taiwan have found that intravenous injections of stem cells derived from human exfoliated deciduous tooth pulp (SHED) have a protective effect against brain damage from heat stroke in mice. Their finding was safe and effective and so may be a candidate for successfully treating human patients by preventing the neurological damage caused by heat stroke.

The study is published in a future issue of Cell Transplantation and is currently freely available on-line as an unedited early e-pub at: http://www.ingentaconnect.com/content/cog/ct/pre-prints/content-CT1100Tseng.

"Heat stroke deaths are increasing worldwide and heat stroke-induced brain injury is the third largest cause of mortality after cardiovascular disease and traumatic brain injury," said study lead author Dr. Ying-Chu Lin of the Kaohsiung Medical University School of Dentistry, Kaohsiung City, Taiwan. "Heat stroke is characterized by hyperthermia, systemic inflammatory response, multiple organ failure and brain dysfunction."

To investigate the beneficial and potentially therapeutic effects afforded by the protective activities of self-renewing stem cells derived from human exfoliated deciduous teeth, the scientists transplanted SHED into mice that had suffered experimental heat stroke.

According to the research team, these cells have "significantly higher proliferation rates" than stem cells from bone marrow and have the added advantages of being easy to harvest and express several growth factors, including vascular endothelial growth factor (VEGF), and they can promote the migration and differentiation of neuronal progenitor cells (NPCs).

"We observed that the intravenous administration of SHED immediately post-heat stroke exhibited several therapeutic benefits," said Dr. Lin. "These included the inhibition of neurological deficits and a reduction in oxidative damage to the brain. We suspect that the protective effect of SHED may be related to a decreased inflammatory response, decreased oxidative stress and an increase in hypothalamo-pituitary-adrenocortical axis activity following the heat stroke injury."

There are currently some drawbacks to the experimental therapy, said the researchers. First, there is a limited supply of SHED. Also, SHED transplantation has been associated with cancer and immune rejection.

Read more from the original source:
Future heat stroke treatment found in dental pulp stem cells