Category Archives: Wyoming Stem Cells

Scientists have created an electronic wafer that reprogrammed damaged skin cells on a mouse's leg to grow new blood vessels and help a wound heal.

One day, creator Chandan Sen hopes, it could be used to be used to treat wounds on humans. But that day is a long way off as are many other regeneration technologies in the works. Like Sen, some scientists have begun trying to directly reprogram one cell type into another for healing, while others are attempting to build organs or tissues from stem cells and organ-shaped scaffolding.

But other scientists have greeted Sen's mouse experiment, published in Nature Nanotechnology on Monday, with extreme skepticism. "My impression is that there's a lot of hyperbole here," says Sean Morrison, a stem cell researcher at the University of Texas Southwestern Medical Center. "The idea you can [reprogram] a limited number of cells in the skin and improve blood flow to an entire limb I think it's a pretty fantastic claim. I find it hard to believe."

When the device is placed on live skin and activated, it sends a small electrical pulse onto the skin cells' membrane, which opens a tiny window on the cell surface. "It's about 2 percent of the cell membrane," says Sen, who is a researcher in regenerative medicine at Ohio State University. Then, using a microscopic chute, the chip shoots new genetic code through that window and into the cell where it can begin reprogramming the cell for a new fate.

Sen says the whole process takes less than 0.1 seconds and can reprogram the cells resting underneath the device, which is about the size of a big toenail. The best part is that it's able to successfully deliver its genetic payload almost 100 percent of the time, he says. "No other gene delivery technique can deliver over 98 percent efficiency. That is our triumph."

To test the device's healing capabilities, Sen and his colleagues took a few mice with damaged leg arteries and placed the chip on the skin near the damaged artery. That reprogrammed a centimeter or two of skin to turn into blood vessel cells. Sen says the cells that received the reprogramming genes actually started replicating the reprogramming code that the researchers originally inserted in the chip, repackaging it and sending it out to other nearby cells. And that initiated the growth of a new network of blood vessels in the leg that replaced the function of the original, damaged artery, the researchers say. "Not only did we make new cells, but those cells reorganized to make functional blood vessels that plumb with the existing vasculature and carry blood," Sen says. That was enough for the leg to fully recover. Injured mice that didn't get the chip never healed.

When the researchers used the chip on healthy legs, no new blood vessels formed. Sen says because injured mouse legs were was able to incorporate the chip's reprogramming code into the ongoing attempt to heal.

That idea hasn't quite been accepted by other researchers, however. "It's just a hand waving argument," Morrison says. "It could be true, but there's no evidence that reprogramming works differently in an injured tissue versus a non-injured tissue."

What's more, the role of exosomes, the vesicles that supposedly transmit the reprogramming command to other cells, has been contentious in medical science. "There are all manners of claims of these vesicles. It's not clear what these things are, and if it's a real biological process or if it's debris," Morrison says. "In my lab, we would want to do a lot more characterization of these exosomes before we make any claims like this."

Sen says that the theory that introduced reprogramming code from the chip or any other gene delivery method does need more work, but he isn't deterred by the criticism. "This clearly is a new conceptual development, and skepticism is understandable," he says. But he is steadfast in his confidence about the role of reprogrammed exosomes. When the researchers extracted the vesicles and injected them into skin cells in the lab, Sen says those cells converted into blood vessel cells in the petri dish. "I believe this is definitive evidence supporting that [these exosomes] may induce cell conversion."

Even if the device works as well as Sen and his colleagues hope it does, they only tested it on mice. Repairing deeper injuries, like vital organ damage, would also require inserting the chip into the body to reach the wound site. It has a long way to go before it can ever be considered for use on humans. Right now, scientists can only directly reprogram adult cells into a limited selection of other cell types like muscle, neurons and blood vessel cells. It'll be many years before scientists understand how to reprogram one cell type to become part of any of our other, many tissues.

Still, Morrison says the chip is an interesting bit of technology. "It's a cool idea, being able to release [genetic code] through nano channels," he says. "There may be applications where that's advantageous in some way in the future."

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A Chip That Reprograms Cells Helps Healing, At Least In Mice - Wyoming Public Media

Mallory Family Wellness offers stem cell therapy for erectile dysfunction for the residents of Loveland, Fort Collins, and the surrounding Northern Colorado communities.

ED is a mans inability to consistently produce or maintain an erection during sexual activity. Most commonly it is caused by a physical issue. Some disease related issues which can cause erectile dysfunction include: heart disease, diabetes, prostate or kidney disease, neurological disorders or blood vessel disease. Other physical causes include: surgery, injury, hormone imbalance, enlarged prostate, tobacco, drugs, alcohol or stress. In most cases it is not simply mind over matter.

ED is something many men do not like to talk about. At Mallory Family Wellness, we make it our business to talk to our patients about health concerns of all types. Our number one goal is treating the whole person and not just the symptoms. ED is nothing to be embarrassed about and is a condition we take seriously at Mallory Family Wellness.

ED can be treated successfully using stem cell therapy. Stem cells are harvested from your own body fat. Once harvested, the stem cells are purified and then infused back into the body through an intravenous (into a vein) injection. For ED, injections are also performed directly into the penis. The infused stem cells are able to transform into endothelial cells causing a secretion of vasculotrophic and neurotrophic factors. Treating erectile dysfunction with stem cell therapy increases blood flow and pressure which means a firmer, more sustainable erection. To find out more about this safe and affordable therapy contact Mallory Family Wellness at 970-669-9245.

The cost of your stem cell therapy is dependent upon your individual condition, nature of the procedure needed, and responsiveness to the therapy. Dr. Mallorys staff will discuss stem cell injection therapy prices with you on an individual basis. With regards to the therapy, it is not covered by insurance, as its categorized with other transplant type therapies and is considered investigational. It is typically eligible for coverage out of a Healthcare Savings Account (HSA) or Healthcare Retirement Account (HRA).

Payment for the procedure may be made by cash, check or HSA/HRA credit cards*. Mallory Family Wellness also works with CareCredit to provide financing options for our patients. You can be pre-approved for CareCredit financing of your procedure within a few hours.

*Patients should verify this eligibility.

Dr. Mallory specializes in regenerative medicine; visit our other stem cell therapy pages including asthma, COPD, ischemic limb disorders, and ortho-arthritic joint injury.

At Mallory Family Wellness, you are our priority. With advanced training in Osteopathic Manipulative Medicine, we dont just treat the symptoms. We treat the whole person to address the root of the problem. And well work with you so you can prevent illness and remain healthy.

See for yourself why families from Loveland, Fort Collins, and the surrounding Northern Colorado communities, as well as Wyoming, North Dakota, South Dakota, Kansas, Nebraska, and Iowa, come to us. You can count on us to provide optimal care for your optimal health!

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Erectile Dysfunction in Loveland, CO

If you are not looking for Peritoneum Cancer locally, check out the popular Peritoneum Cancer brands on the right navigation area of the page. On the Peritoneum Cancer pages we include website links so you can check out Peritoneum Cancer online.

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If you are open to the idea of adult stem cell treatment and adult stem cell therapy as an alternative method of treatment for your medical disorder Peritoneum Cancer, then you have found the right place. Here at naturalcurefor.com we specialize in helping people heal by providing effective and natural cures for Peritoneum Cancer in or near Wyoming, New York 14591. For immediate, free, and confidential assistance, download our .pdf file and call our helpline NOW!

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Wyoming, New York - Natural Cure for Peritoneum Cancer - We Can Provide Some Tremendous Hope if You Are Willing to Consider Adult Stem Cell Therapy and Treatments as Your Path Back To Good Health

Treating Peritoneum Cancer in a traditional medical manner is sometimes a long and grueling process that can offer less hope than you deserve. Additionally, many traditional medical treatments are riddled with drugs and medications that can sometimes cause even more harm to other parts of the body. Further risks of medication mixups, allergies, destruction of the immune system and the constant level of additional medical treatments that may be required, can sometimes weigh heavily on a patient and their chances of regaining a healthful way of life. Recovery can become difficult or almost impossible in some cases.

We offer an alternative treatment or a more natural cure process that centers around the idea of using your own adult stem cells as the basis of this natural treatment. In some areas of the country, traditional medicine and medical practices may not have acknowledged the benefits that stem cell treatments can bring to the healing process. Stem cell treatments may not be a standard course of medical treatment quite yet, but that may be a result of other political and/or profit motives. But rest assured that is all changing and changing quite rapidly as more and more success and overwhelming evidence indicates that adult stem cell therapy is a very successful and viable treatment process for Peritoneum Cancer.

Stem cell treatment is extremely effective and very safe. It is also very natural, ethical and a very effective way in assisting the body to heal naturally and wholesomely. It embodies the very idea of "healing" rather than simply medicating a symptom. The main idea of how adult stem cell treatments work are as follows...

Adult stem cells are in your body right now. Yes, everybody has them. These are cells that have not yet differentiated themselves quite yet or basically stated they have not yet made up their mind as to what type of cells they are going to be. In other words, normal adult stem cells are a bit of a blank slate, ready to assume the impression of whatever task or needs the body may have for them.

Stem cell treatment involves the process of simply removing some of these adult stem cells from your own body - usually out of the side of your body - and then placing them in a petrie dish and adding enzymes to make them multiply very rapidly. After an incubation period of time, the stem cells are then reinserted back into you body directly into the afflicted area or in the area of Peritoneum Cancer - that is in the exact position and location where the adult stem cells are needed.

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Natural cures for Peritoneum Cancer in Wyoming New York ...

Stem Cell Breast Augmentation fees estimate between $18,000.00 to $22,000.00. There are several factors that determine the fee schedule such as the number of areas being treated, procedure time and overall goals.

Click Here to Read FAQ's

Dr Malan's Stem Cell Enhanced Natural Breast Augmentation uses a patients's fat tissue, which is a rich source of stem cells and regenerative cells, to provide women the option of enlarging their breast without the use of unnatural implants. These stem cells are not the controversial embryonic stem cells.

First, the Body-Jet liposuction device utilizes streaming water to gently harvest the fat. The fat is then processed to extract and activate the stem cells. The stem cells are then concentrated with the fat and injected in the breast. The injection of the fat along with the stem cells results in a permanent 250cc to 500cc overall breast size increase, which is a 1 or 2 bra cup size increase. A pressure splint is sometimes used after the procedure to ensure that the fat remains in place and assists in promoting regeneration of the transferred fat and stem cells.

The end result is enlarged breasts that are very natural in appearance and feel without the complications associated with implants. Unlike traditional implants, this procedure will not interfere with future Mammograms or breastfeeding. The technique does not require general anesthesia, typically requires only 48 hour recovery period and leaves permanent results.

Dr. Todd Malan - First in U.S. to perform Stem Cell Enhanced Breast Augmentation! READ FULL ARTICLE

Using Stem Cells in Breast Augmentation - Interview with Dr. Todd Malan READ FULL ARTICLE

Q: I have heard that this procedure is controversial? A: For 20 years the major U.S. Cosmetic Surgery society denounced Fat Transfer to the Breast as a dangerous procedure. This decision was based on technology and techniques used 20 years ago. Recent developments and results from our patients prompted the recent lift on this ban in March 2009. Many Cosmetic Surgeons are unaware of the new and improved techniques or of the recent lifting of this ban and still advise patients against this procedure. The common myths you will hear from doctors who have not followed these recent changes are;

Q: Who is the ideal candidate? A: Anyone who desires a permanent increase in breast size that is natural in appearance. The Natural Breast Augmentation is ideal for the person who has lost fullness in her breast due to age, weight fluctuations, and breast feeding. A 1-2 cup size increase and more fullness are expected from this procedure. However the person who would not be happy with anything less that a 2-3 cup size increase should consider Breast Implants.

Q: I read most of the fat doesn't survive so more than one surgery is needed? A: New techniques have proven an 85-90% fat survival. Stem cell techniques in Europe and Japan have shown fat survival to be even higher. The alternative breast implants need to be replaced every 10 years and problems such as scarring and rupture are common.

Q: I heard calcifications caused by the surgery can interfere with mammograms? A: Studies have proven that newer digital mammograms can easily evaluate calcifications in the breast whereas the alternative enhancement procedure, Breast Implants, block more than half of the breast from the view of mammograms.

Q: Is this similar to the Stem Cell Breast Augmentations being used in Japan? A: Yes, we offer stem cell enhanced fat transfer which has been proven in Europre and Japan to improve fat survival.

Q: Why should I chose Dr. Todd Malan? A: Since the recent lifting of the ban on this procedure many doctors have begun offering fat transfer to the breast surgery. The lift on the ban was due in part to Dr. Malan's improved technique and Dr. Malan trains doctors world wide in his procedure. In fact Dr. Malan is the first certified training course for doctors in the U.S. Unless the doctor has been trained by Dr. Malan they are likely using old techniques that caused the ban 20 years ago.

Q: What if I don't really need liposuction but still want a Natural Breast (Stem Cell) Augmentation? A: Dr. Todd Malan is dedicated to not only giving you natural looking breasts but also great liposuction results. Patients travel from all over the world to have their liposuction procedure performed by Dr. Malan. The stem cell procedure allows Dr. Malan to use far less fat to achieve great results.

1: Body-Jet Water Liposuction gently removes fat, regenerative, and stem cells from abdomen. 2: Cells go into the Stem Cell processing system. 3: The Stem Cell processing system separates regenerative and stem cells from fat cells. The regenerative and stem cells are then washed and concentrated. 4: The concentrated stem cell mixture is combined with the fat cells. 5: The final mixture is injected into the breast, where it replaces tissue volume.

The Stem Cells when injected may the help to:

*Regenerative and Stem Cell information presented is based on clinical studies conducted in Europe and Asia. Current U.S. studies are underway.

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Doctors in Wyoming who treat or diagnose Stem Cell Transplant

siRNA-mediated Knockdown of PDGF R alpha, PDGF R beta, and cAbl Upregulates Expression of Pluripotent, Mesoderm, and Endoderm Markers. Small interfering RNAs (siRNAs) were transfected into mesenchymal stem cells by electroporation. Knockdown was verified by RT-PCR and Western blot analysis (data not shown here). Cell lysates from siRNA-treated cells were used in the Proteome Profiler Human Pluripotent Stem Cell Antibody Array (Catalog#ARY010) to examine relative expression levels of mesoderm, endoderm, and pluripotent markers. The images represent nitrocellulose membranes following chemiluminescent detection of bound analytes. Each of the array markers has duplicate spots, which are boxed to highlight their identity. A. Scrambled (Scr) siRNA-treated mesenchymal stem cells. B. Knockdown of PDGF R alpha upregulated pluripotent markers (Oct-3/4, Nanog, SOX2, and E-Cadherin) as well as markers of the mesoderm and endoderm compared to Scr. C. Knockdown of PDGF R beta upregulated expression of Snail, SOX17, VEGF R2, Oct-3/4, and Nanog compared to both Scr and PDGF R alpha knockdowns. D. Knockdown of cAbl upregulated pluripotent markers (Nanog, Oct-3/4, E-Cadherin, and SOX2) compared to Scr in addition to markers of the endoderm and mesoderm.

Adapted from Ball, S.G. et al. (2012) Stem Cells 30:548.

Abbreviations: AFP, alpha-Fetoprotein; GAPDH, Glyceraldehyde 3-Phosphate Dehydrogenase; GSC, Goosecoid; HCG, Human Chorionic Gonadotropin; HNF, Hepatocyte Nuclear Factor; PDGF R, Platelet-derived Growth Factor Receptor; Scr, scrambled; VEGF R, Vascular Endothelial Growth Factor Receptor.

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Proteome Profiler Human Pluripotent Stem Cell Array Kit ...

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Source: Research by Tim Friend and Dan Vergano, USA TODAY By Frank Pompa and Julie Snider, USA TODAY

Although the general public considers stem cell therapy an innovative, cutting-edge treatment, the fact is that this kind of therapy already has a lengthy history. In the past, however, stem cells were difficult and very expensive to obtain. Luckily, the advent of improved equipment and techniques has meant that stem cells can now be acquired through a simple procedure.

Stem cells can be characterized as the bodys repairmen. The most common type is the hematopoietic stem cell (HSC-CD 34+). The old thinking was that the hematopoietic stem cells were not that important. We now know that these are the cells that are the true drivers of tissue regeneration. The good news about these cells is that their numbers do not diminish with age. The other type of stem cell associated with tissue healing is the mesenchymal stem cell (MSC), which usually travels to injured areas of the body via the bloodstream. The mesenchymal stem cell is still a very important stem cell but not as important as it once was. It prepares the area for the other stem cells to do their work.

If the area in question has an insufficient blood supply, this is termed an area of hypoxia, otherwise known as low oxygen content. Hypoxia areas can include the rotator cuff, the joints, meniscus tissue, and other spots with tendon injuries. Typically, these areas are unable to heal properly without help, as the body does not send enough repair cells to the afflicted areas. The inadequate supply of blood in these areas means that the body fails to sense the injury. Fortunately, we are generally able to treat the area, if the injury isnt severe, with platelet-rich plasma. This works by effectively mimicking a blood supply, allowing the platelets to sense the injury and release growth factors, which then prompt the body to send various stem cells to the area.

Stem cells are gathered by aspirating (removing through suction) bone marrow from the back of a patients pelvis. This bloody substance is removed from the patients pelvis with a tiny needle. Since the patient is given a local anesthetic, only minimal discomfort results from the procedure.

In most cases, 2 oz. (60 cc) of bone marrow aspirate is required. The aspirate includes platelets, mesenchymal stem cells, and other kinds of stem cells used in adult stem cell therapy. After aspiration, the bone marrow is placed inside a special container, which in turn is placed into a machine known as a centrifuge. The centrifuge spins the material at a high rate of speed, and this process separates the platelets and stem cells from the remainder of the blood products. It is this concentration of bone marrowcalled BMAC, or bone marrow aspiration concentratethat is reintroduced to the injured area during stem cell therapy.

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