Stem Cell Therapy – Boulder, Colorado | Boulder Biologics |Jason Glowney, MD | Stem Cells – Denver, Colorado | PRP | Regenerative Medicine | EPAT |…

Typically, a hybrid bone marrow stem cell therapy protocol is used. In this protocol, a small amount of the filtered bone marrow aspirate is set aside and not processed, but the majority of the total bone marrow aspirate is centrifuged to concentrate the nucleated cells with stemness potential. Stem cells numbers are important, but we might be discarding important healing agents like pluripotent Muse cells and very small embryonic-like stem cells (VSELs), that have even greater potential for healing than MSCs.

Historically, for the bone marrow concentration technique, most of the emphasis was put on increasing the density of nucleated cells. Many of the techniques employed by stem cell providers and bedside kits use centrifuge RPMs and g-forces that are extremely high. In our testing, we found that these excessively high forces come at a steep price in terms of the end result for the viability of the cells that are isolated. This has led us to employ a much safer approach to our cellular isolation techniques. We see below in Fig 2., a Wrights stained peripheral smear of our bone marrow concentrate after centrifugation with a higher density of nucleated cells.

Original post:
Stem Cell Therapy - Boulder, Colorado | Boulder Biologics |Jason Glowney, MD | Stem Cells - Denver, Colorado | PRP | Regenerative Medicine | EPAT |...