Induced Pluripotent Stem Cell Services Induced …

Background

The recent discovery of reprogramming human somatic cells into induced pluripotent stem cells (iPS) offers an innovative approach to the study of many human genetic diseases. Like human embryonic stem cells, iPS cells self-renew indefinitely and have unlimited developmental potential. The creation of patient-specific iPS cells holds great promise for understanding genetic disease mechanisms and for in vitro testing of potential therapies. University of Connecticut induced Pluripotent Stem Cell Core (iPSCC) has extensive experiences in deriving iPSCs for many scientists from both academia and industry. We will continue to provide a high quality, reliable, and efficient reprogramming services to the increasing demands at University of Connecticut and elsewhere, both statewide and nationwide.

We currently produce in-house retrovirus for six factors total, four factors, originally developed in Yamanaka lab, including OCT4, SOX2, C-MYC, and KLF4 and two additional factors, NANOG and LIN28, which were used in Thomson lab for reprogramming. In addition, we produce lentivirus using a single polycistronic lentiviral vector (STEMCCA) to improve efficiency and reduce the number of viral integrations. Furthermore, this vector includes the flanking LoxP sites to facilitate Cre-mediated excision of the vector later if necessary Finally, we are in the process of optimizing the integration-free reprogramming protocols such mRNA transduction and episomal approach.

To initiate this service, please contact the iPSCC at ucscicore@uchc.edu and submit using the the sample submission form. We would be happy to answer questions about our iPSCC derivation service. Once arrangements between client and iPSCC are made, the client can submit the sample to us. Clients can submit either frozen or live cultures.

2) Derivation of iPSC Includes:

iPSCC Validation Service: Validation includes, but not limited to, immunocytochemistry, mycoplasma testing, and teratoma service.

We test NANO and SSEA4 immunostaining for one hESC or iPSC line, or 1 to 5 iPSC lines derived from the same fibroblast sample plate as an add-on to the iPSC derivation service. This service may be ordered separately from our iPSC derivation service (e.g., for other cells), if desired.

b. Mycoplasma Testing:

Mycoplasma test. The University of Connecticut iPSCC provides a sensitive and accurate biochemical mycoplasma test using Myco-Alert kit. Clients submit 2 ml of the spent culture medium to be tested. We request to fill out a separate sample submission form before you request the test online.

c. Teratoma Service:

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Induced Pluripotent Stem Cell Services Induced ...