Tristan Darland | Faculty | Biology | Arts & Sciences …

Assistant Professor of Biology

Starcher Hall Room 7

Office Tel: 701.777.3375

tristan.darland@UND.edu

B.S., 1986, Biology, Revelle College, University of California, San Diego, CA

Ph.D., 1998, Cell and Developmental Biology, Oregon Health Sciences University, Portland, OR

Visiting Faculty Member, Turtle Mountain Community College, Belcourt, ND

Instructor and Research Professor, Department of Biology, University of North Dakota

Postdoctoral Research Associate, Harvard University, Boston, MA

I am interested in finding new genes, or new functions of known genes, involved in diverse biological processes ranging from neurodevelopment and neural stem cell regulation to addiction related behavior. I use zebrafish because the genetic analysis possible is amenable to find new candidate genes and is not currently possible in other vertebrates. I am currently identifying the genes underlying mutations that affect neural stem cell regulation in the retina. Fish have the ability to generate new nervous tissue in adulthood, a process that may be applicable to treating neural injury and pathology in humans. Also, I am identifying other mutant gene products that underlie abnormal behavioral responsiveness to addictive drugs.

I house a number of wild type strains of zebrafish and hope to make the organism available to other investigators on the UND campus interested in trying new experiments with this very useful model system. In addition, I am involved in summer educational outreach programs for undergraduates and hope to provide teaching laboratory workshops on using zebrafish for genetic analysis. Undergraduates conducting research in my laboratory are encouraged to participate in all levels of the research endeavor and to present their findings at local, regional, and national meetings. Ana Espinoza (Arizona Western College) and Joclyn Seiler (University of North Dakota) recently presented their research results at the UND REU-Neuroscience poster session (August, 2012) and again at the North Dakota EPSCoR meeting (September, 2012).

Mersereau, E.J., Boyle, C.A., Poitra, S., Espinoza, A., Seiler, J., Longie, R., Delvo, L., Szarkowski, M., Maliske, J., Chalmers, S., Darland, D.C., and Darland, T. Longitudinal effects of embryonic exposure to cocaine on morphology, cardiovascular physiology, and behavior in zebrafish. International Journal of Molecular Sciences, 2016 May 17:847-865; [doi:10.3390/ijms17060847] Special Note: This is an issue dedicated to zebrafish toxicology models.Special Note: This is an issue dedicated to zebrafish toxicology models.

Mersereau EJ, Poitra SL, Espinoza A, Crossley DA 2nd, Darland T. The effects of cocaine on heart rate and electrocardiogram in zebrafish (Danio rerio); Comp Biochem Physiol and Toxicol Pharmacol. 2015; Jun-Jul; 172-173;1-6; PMID: 25847362.

Darland, T., Mauch, J.T., Meier, E.M., Hagan, S.J., Dowling, J.E., and Darland, D.C. Sulpiride, but not SCH23390, modifies cocaine-induced conditioned place preference and expression of Tyrosine hydroxylase and Elongation factor 1 in zebrafish. Pharmacology, Biochemistry, and Behavior 2012; 103(2):157-167.PMID: 22910534

Darland, D.C., Cain, J.T., Berosik, M.A., Saint-Geniez, M., Odens, P.W., Schaubhut, G.J., Frisch, S., Stemmer-Rachamimov, A., Darland, T., and D'Amore, P.A. Vascular endothelial growth factor (VEGF) isoform regulation of early forebrain development. Developmental Biology 2011; 358(1):9-22.PMID: 21803034

Nuckels, R.J., Ng, A. Darland, T. and Gross, J.M. The Vacuolar-ATPase Complex Regulates Retinoblast proliferation and Survival, Photoreceptor Morphogenesis and Pigmentation in the Zebrafish Eye. Investigative Ophthalmology and Visual Sciences 2009; 50(2): 893-905.PMID: 18836173

Gross JM, Perkins BD, Amsterdam A, Egana A, Darland T , Matsui JI, Sciasscia S, Hopkins N and Dowling JE. Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics 2005; 170(1):245-61.PMID: 15716491

Darland T and Dowling JE. Behavioral screening for cocaine sensitivity in mutagenized zebrafish. PNAS USA 2001; 98(20): 11691-6.PMID: 11553778

Link BA and Darland T . Genetic analysis of initial and ongoing retinogenesis in the zebrafish: comparing the central neuroepithelium and marginal zone. Prog Brain Res . 2001; 131: 565-77.PMID: 11420971

Bunzow JR, Sonders MS, Arttamangkul S, Harrison LM, Zhang G, Quigley DI, Darland T , Suchland KL, Pasumamula S, Kennedy JL, Olsen SB, Magenis RE, Amara SG and Grandy DK. Amphetamine, 3,4-methylenedioxymethamphetamine, lysergic acid diethylamide, and metabolites of the catecholamine neurotransmitters are agonists of a rat trace amine receptor . Mol. Pharm. 2001; 60 (6) 1181-8.

Allen CN, Jiang ZG, Teshima K, Darland T , Ikeda M, Nelson CS, Quigley DI, Yoshioka T, Allen RG, Rea MA and Grandy DK. Orphanin-FQ/nociceptin (OFQ/N) modulates the activity of suprachiasmatic nucleus neurons. J. Neurosci. (1999); 19(6): 2152-60.

Darland T , Grandy DK. The orphanin FQ system: an emerging target for the management of pain? Br. J. Anaesth . 1998; 81(1): 29-37.

Darland T , Heinricher MM, Grandy DK. Orphanin FQ/Nociceptin: a role in pain and analgesia, but so much more. Trends in Neurosci . 1998; 21(5): 215-21.

Quigley DI, McDougall J, Darland T , Zhang G, Ronnekliev O, Grandy DK, Allen RG. Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus. Peptides 1998;19(1):133-9.

Darland T , Leblanc GG. Immortalized Hensen's node cells secrete a factor that regulates avian neural crest cell fates in vitro . Dev Biol . 1996; 176(1): 62-75.

Leblanc GG, Holbert TE, Darland T . Role of the transforming growth factor-beta family in the expression of cranial neural crest-specific phenotypes. J Neurobiol . 1995; 26(4): 497-510.

Huang RP, Darland T , Okamura D, Mercola D, Adamson ED. Suppression of v-sis-dependent transformation by the transcription factor, Egr-1. Oncogene 1994; 9(5):1367-77.

Joh T, Darland T , Samuels M, Wu JX, Adamson ED. Regulation of epidermal growth factor receptor gene expression in murine embryonal carcinoma cells . Cell Growth Differ. 1992;3(5):315-25.

Edwards SA, Darland T , Sosnowski R, Samuels M, Adamson ED. The transcription factor, Egr-1, is rapidly modulated in response to retinoic acid in P19 embryonal carcinoma cells. Dev. Biol . 1991; 148(1): 165-73.

Darland T , Samuels M, Edwards SA, Sukhatme VP, Adamson ED. Regulation of Egr-1 and c-fos expression in differentiating embryonal carcinoma cells. Oncogene 1991; 6(8):1367-76.

Calogero A, Samuels M, Darland T , Edwards SA, Kemler R, Adamson ED. Overexpression of uvomorulin in a compaction-negative F9 mutant cell line. Dev Biol. 1991;146(2):499-508.

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