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Largest Map of Protein Connections Holds Clues to Health and Disease – Technology Networks

Posted: April 12, 2020 at 6:42 pm

The human body is composed of billions of cells, each of which is made and maintained through countless interactions among its molecular parts. But which interactions sustain health and which ones can cause disease when they go awry?

The human genome project has provided us with a parts list for the cell, but only if we can understand how these parts go together, or interact, can we really begin to understand how the cell works and what goes wrong in disease.

To answer these questions, scientists needed a reference map of interactionsan interactome between gene-encoded proteins, which make up cells and do most of the work in them.

Almost a decade in the making, the human protein map is now available thanks to a joint effort, involving over 80 researchers in the United States, Canada, Spain, Belgium, France and Israel, led byMarc Vidal,David HillandMichael Calderwood, at the Center for Cancer Systems Biology (CCSB) at Dana-Farber Cancer Institute, andFrederick Roth, a professor of molecular genetics and computer science at U of Ts Donnelly Centre.

The largest of its kind, the human reference interactome (HuRI) map charts 52,569 interactions between 8,275 human proteins, as described in astudypublished inNature.

Humans have about 20,000 protein-coding genes but scientists still know remarkably little about most of the proteins they encode. Fortunately, this information can be gleaned from interaction data thanks to the guilt by association principle, according to which two proteins that have similar interacting partners are likely involved in similar biological processes.

People can look up their favourite protein and get clues about its function from the proteins it interacts with.

The data are already revealing new cellular roles for human proteinsinvolved in programmed cell death, release of cellular cargo and other essential processes, for example.

And, by integrating protein interaction data with tissue-specific gene expression, the teams have been able to identify protein networks behind the development and maintenance of different tissues, revealing new therapeutic targets for diverse diseases.

Furthermore, using HuRI as a reference, they were also able to see how disease-causing protein variants bring about network rewiring to reveal molecular mechanisms behind those particular disorders.

Genome sequencing can identify the variants carried by an individual that make them susceptible to disease, but it doesnt reveal how the disease is caused, says Calderwood. Changes in the interactions of a protein is one possible mechanism of disease, and this map provides a starting point to study the impact of disease associated variants on protein-protein interactions.

The Toronto and Boston teams previously did two smaller studies mapping a total of ~14,000 protein interactions. Now HuRI has interrogated proteins encoded by nearly all human protein-coding genes and expanded the map four-fold.

To create HuRI, the researchers co-expressed in pairs almost all human proteins in yeast cells. When the two proteins interact, or bind one another, they form a molecular switch which boosts yeast cell growtha sign that an interaction has occurred.

The team tested all possible pairwise combinations among 17,500 proteins for their ability to interact with each other in three separate versions of a yeast-based assay, each done in triplicate, amounting to a staggering three billion separate tests. The results yielded ~53,000 high-confidence binary interactions between more than 8,000 proteins, which were verified by other methods. The majority of interactions had never been detected before.

Although the largest map of its kind to date, the map remains incomplete, representing between 2-11 per cent of all human protein interactions. Roth said that one reason why many interactions were missed is probably because yeast cells lack certain human-specific molecular factors that are needed for proper protein function.

Despite these limitations, HuRI has more than tripled the number of known interactions between human proteins and will serve as an important resource for the research community. Already 15,000 people have visited the data web portal, which was built byMiles Mee,Mohamed Helmy, andGary Bader, also a professor in the Donnelly Centre, since HuRI was made available on bioRxiv, an open-source online publisher, in April 2019.

We already had lots of people download the whole dataset and so I imagine well see the iteration of our previous paper, which has already been cited over 800 times and it is less than a third of the size of HuRI, says Roth.

The research was primarily supported by the National Institutes of Healths National Human Genome Research Institute, but had additional support internationally from other sources.

Reference: Luck et al. (2020).A reference map of the human binary protein interactome. Nature.DOI: https://doi.org/10.1038/s41586-020-2188-x.

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.

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Myriad Withdrawing Financial Guidance for FY2020 Due to Business Impact from Coronavirus Pandemic – Yahoo Finance

Posted: April 12, 2020 at 6:42 pm

SALT LAKE CITY, April 08, 2020 (GLOBE NEWSWIRE) -- Myriad Genetics, Inc. (MYGN), a global leader in personalized medicine, announced today that due to the impact of the global COVID-19 pandemic, the company is withdrawing its fiscal year 2020 financial guidance.

Prior to mid-March we were experiencing volume trends consistent with our expectations across all products; however, recent social distancing guidelines have had a significant impact on test volume trends in late March and into the fiscal fourth-quarter, said R. Bryan Riggsbee, interim president and CEO and chief financial officer at Myriad Genetics. Our priority as an organization during the coronavirus pandemic has been to maintain business continuity and access to testing, while ensuring the safety of our employees and customers. As an organization we have taken steps to advance these dual aims, and I am very proud of how the Myriad team has responded to the crisis.

In responding to the pandemic, Myriad has made several changes to its business practices to promote the safety of both customers and employees including ceasing in-office sales calls and implementing virtual selling, granting all non-essential personnel the ability to work from home, enabling direct sample collection for patients and implementing policies to improve laboratory personnel safety.

While the uncertain timeframe of the Coronavirus pandemic makes it difficult to predict future business trends for the company, the company will provide an update on its business, including the impact of COVID-19, on its next quarterly earnings call.

About Myriad GeneticsMyriad Genetics, Inc. is a leading personalized medicine company dedicated to being a trusted advisor transforming patient lives worldwide with pioneering molecular diagnostics. Myriad discovers and commercializes molecular diagnostic tests that determine the risk of developing disease, accurately diagnose disease, assess the risk of disease progression, and guide treatment decisions across six major medical specialties where molecular diagnostics can significantly improve patient care and lower healthcare costs. Myriad is focused on three strategic imperatives: transitioning and expanding its hereditary cancer testing markets, diversifying its product portfolio through the introduction of new products and increasing the revenue contribution from international markets. For more information on how Myriad is making a difference, please visit the Company's website: http://www.myriad.com.

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Myriad, the Myriad logo, BART, BRACAnalysis, Colaris, Colaris AP, myPath, myRisk, Myriad myRisk, myRisk Hereditary Cancer, myChoice, myPlan, BRACAnalysis CDx, Tumor BRACAnalysis CDx, myChoice HRD, Vectra, Prequel, ForeSight, GeneSight and Prolaris are trademarks or registered trademarks of Myriad Genetics, Inc. or its wholly owned subsidiaries in the United States and foreign countries. MYGN-F, MYGN-G.

Safe Harbor StatementThis press release contains "forward-looking statements" within the meaning of the Private Securities Litigation Reform Act of 1995, including statements relating to maintaining the Companys global leadership in precision medicine and the Company's strategic directives under the caption "About Myriad Genetics." These "forward-looking statements" are based on management's present expectations of future events and are subject to a number of risks and uncertainties that could cause actual results to differ materially and adversely from those described or implied in the forward-looking statements. These risks include, but are not limited to: the risk that sales and profit margins of the Companys existing molecular diagnostic tests and pharmaceutical and clinical services may decline or will not continue to increase at historical rates; risks related to the Companys ability to successfully transition from its existing product portfolio to our new tests; risks related to changes in the governmental or private insurers reimbursement levels for the Companys tests or the Companys ability to obtain reimbursement for its new tests at comparable levels to its existing tests; risks related to increased competition and the development of new competing tests and services; the risk that the Company may be unable to develop or achieve commercial success for additional molecular diagnostic tests and pharmaceutical and clinical services in a timely manner, or at all; the risk that the Company may not successfully develop new markets for its molecular diagnostic tests and pharmaceutical and clinical services, including the Companys ability to successfully generate revenue outside the United States; the risk that licenses to the technology underlying the Companys molecular diagnostic tests and pharmaceutical and clinical services tests and any future tests are terminated or cannot be maintained on satisfactory terms; risks related to delays or other problems with operating the Companys laboratory testing facilities; risks related to public concern over the Companys genetic testing in general or the Companys tests in particular; risks related to regulatory requirements or enforcement in the United States and foreign countries and changes in the structure of the healthcare system or healthcare payment systems; risks related to the Companys ability to obtain new corporate collaborations or licenses and acquire new technologies or businesses on satisfactory terms, if at all; risks related to the Companys ability to successfully integrate and derive benefits from any technologies or businesses that it licenses or acquires; risks related to the Companys projections about the potential market opportunity for the Companys products; the risk that the Company or its licensors may be unable to protect or that third parties will infringe the proprietary technologies underlying the Companys tests; the risk of patent-infringement claims or challenges to the validity of the Companys patents; risks related to changes in intellectual property laws covering the Companys molecular diagnostic tests and pharmaceutical and clinical services and patents or enforcement in the United States and foreign countries, such as the Supreme Court decisions Mayo Collab. Servs. v. Prometheus Labs., Inc., 566 U.S. 66 (2012), Assn for Molecular Pathology v. Myriad Genetics, Inc., 569 U.S. 576 (2013), and Alice Corp. v. CLS Bank Intl, 573 U.S. 208 (2014); risks of new, changing and competitive technologies and regulations in the United States and internationally; the risk that the Company may be unable to comply with financial operating covenants under the Companys credit or lending agreements; the risk that the Company will be unable to pay, when due, amounts due under the Companys credit or lending agreements; and other factors discussed under the heading "Risk Factors" contained in Item 1A of the Companys most recent Annual Report on Form 10-K filed with the Securities and Exchange Commission, as well as any updates to those risk factors filed from time to time in the Companys Quarterly Reports on Form 10-Q or Current Reports on Form 8-K. All information in this press release is as of the date of the release, and Myriad undertakes no duty to update this information unless required by law.

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Researchers at U of T, Sinai Health working on blood test to screen thousands for COVID-19 immunity – News@UofT

Posted: April 12, 2020 at 6:42 pm

A team of researchers at Sinai Health Systemand the University of Toronto is in the early stages of developing a blood test that can identify who is immune to COVID-19 on a mass scale.

The test is an adaptation of an ELISA assay (enzyme-linked immunosorbent assay)and has the potential to enable hospitals and other institutions to screen up to 10,000 samples at once, allowing entire workforces to be tested efficiently.

The blood-based test, whichthe team hopes to test on volunteers within the next two weeks, does not directly detect the live virus and is not intended to replace current tests for infection.

Anne-Claude Gingras, project co-lead, said the test works by detecting antibodies in the immune system of infected patients. Those antibodies persist in blood even after the virus has been completely eliminated.

The entire city has come together to make this possible, said Gingras, a senior investigator at Sinai Healths Lunenfeld-Tanenbaum Research Institute (LTRI) and a professor of molecular genetics at U of T. This test is being developed with the goal of monitoring the percentage of the population that has been infected and to help in identifying those individuals that may have protective immunity.

The project is a collaboration between Gingras and Jeff Wrana, also a senior investigator at LTRI and a professor of molecular genetics at U of T, and other researchers from the Faculty of Medicine.

The team includes James Rini, a professor of biochemistry and molecular genetics who was key to producing proteins for the assay, and Professors Jennifer Gommerman and Mario Ostrowski from the department of immunology, who helped supply samples from pre-pandemic subjects as well as patients infected early in the pandemic who have since recovered.

The new ELISA test can provide valuable information about the spread of SARS-CoV-2 in Canada, said Karen Maxwell, an assistant professor of biochemistry who is helping to co-ordinate COVID-19 research at U of T.

This test will allow us to track the true spread and magnitude of the disease, Maxwell said. Determining who has been infected and has antibodies will be important information for making decisions about how and when we return to our normal activities.

The test will make use of the robotics platform at LTRI. Jim Woodgett, director of research at LTRI and a professor of medical biophysics at U of T, said such advances are possible thanks to close collaboration between scientists across institutions.

Sinai Health and the University of Toronto are ideally positioned to develop this critically important antibody-based test, Woodgett said. This research group is eager to contribute in any way possible to help Canada overcome this historic public health challenge.

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A Deep Look into the Biology and Evolution of COVID-19 – UC San Diego Health

Posted: April 12, 2020 at 6:42 pm

UCTV roundtable explores the biological roots and spread of the global SARS-CoV-2 virus

Of the hundreds of coronaviruses known to exist, many are relatively harmless. Coronaviruses infect your nose, sinuses and upper throat but often result in nothing more than a common cold (see Know Your Coronaviruses).

So what makes the new SARS-CoV-2, the virus that has caused a global pandemic, such a society-altering threat?

Probing the biological basis of the novel virus and evolutionary spread of the COVID-19 disease it causes, a panel of UC San Diego biologists gathered for a special roundtable analysis hosted by UCTV. The program is available here: A Deep Look into the Biology and Evolution of COVID-19.

Roundtable moderator Suresh Subramani, distinguished professor emeritus in the Division of Biological Sciences and director of the Tata Institute for Genetics and Society, framed the program by highlighting three major areas of concern surrounding the pandemic and how it impacts our daily lives: the rapid spread of the virus over the past three months; the ominous morbidity and mortality rates of the disease, which threaten to overwhelm global health care systems; and the immense reservoir of carriers of the disease.

Dissecting COVID-19: Biology Professor Opens Infectious Disease Course to Public Audiences

It is estimated that there may be tenfold more asymptomatic carriers of the disease, which means that there could be over seven-and-a-half million carriers worldwide, said Subramani. This is a disease that is spreading very rapidly across the globe, so these faculty are here to share their knowledge regarding the biology of the virus and why this pandemic has brought the world to its knees.

Emily Troemel, a professor who studies host-pathogen interactions in the Section of Cell and Developmental Biology, kicked off the discussion by describing basic biological aspects of coronaviruses, including how health workers test for the presence of SARS-CoV-2 infection and facets scientists have learned about the virus genome.

Coronaviruses, as Troemel noted, feature RNA-based genomes, unlike most of life on the planet, which feature DNA genomes. RNA genomes in coronaviruses are positive-sense, which are similar to the cells own messenger RNA and allows these viruses to immediately hijack the protein synthesis machinery of host cells. This feature enables these viruses to quickly and effectively take over host cells and rapidly expand.

Knowing that it has RNA in its genome helps us understand how we test for the presence of coronavirus, said Troemel. In addition, we are able to look at changes in the sequence in the viral genome and thats enabling us to track the spread of this virus around the globe. We can learn about how the biology of the virus is changing and how it may be altering the way it interacts with host cells, and also potentially different ways that we could treat it. Its part of an amazing open science effort with an unprecedented level of information acquisition and information sharing among researchers.

Credit: iStock.com/ChakisAtelier

Matt Daugherty, an assistant professor in the Section of Molecular Biology, studies the evolutionary arms race that pits the immune systems of hosts on one hand and pathogens on the other. He covered aspects such as how SARS-CoV-2 and other viruses enter the human population and become pandemics; how SARS-CoV-2 relates to past and present epidemic viruses in the human population; and, based on what scientists have learned from other viruses, what we can expect in terms of long-term immunity and co-existence with SARS-CoV-2.

We as a species are always being exposed to viruses, Daugherty noted.

Since SARS-CoV-2 is so new, there are many key unknowns related to human immune defenses against it, Daugherty said. Even with coronaviruses that cause common colds, its unclear whether humans develop long-term immunity to these viruses or need to continually develop new immunities.

One thing I take comfort in with all of these other viruses is knowing that we arent constantly dealing with influenza pandemics and other pandemic viruses, and thats because of the largely effective role of our immune system in dealing with these viruses once the immune system has been prepared, said Daugherty.

For a virus that originated in an animal species to successfully infect humans, it needs to adapt to a range of genetic differences between the original host species and humans. But effective vaccines can ultimately thwart such pathogens.

We have really good ways of making effective vaccines, and the hope is that this will hold for SARS-CoV-2 as well, said Daugherty. I take some comfort in knowing that these types of pandemics do pass and we will get through this.

Justin Meyer, an assistant professor in the Section of Ecology, Behavior and Evolution, discussed concepts related to science and societys ability to predict future pandemics. These include variables that contribute to the spread of pathogens; the increased likelihood of future pandemics; and predictions for where the next pandemic is likely to occur.

Factors that boost the risk of pandemics include human exposure to pathogens through meat consumption and contact with wild animals, increased human encroachment in wild areas and the exotic animal trade. Increased urbanizationmore people living in close proximity means more opportunities for viruses to spreadand the rising consequences of climate change, also increase pandemic risks.

Were augmenting the temperature of the earth and environments in a way that were making ourselves more susceptible to diseases, said Meyer. When we warm the earth, we create more habitats for mosquitoes that carry vectors like malaria by increasing their range. They can spread to new human populations..... By increasing temperatures, were increasing flooding and there are many pathogens that are waterborne, such as cholera, which we will be exposing more and more people to.

During the roundtable discussion, Subramani prompted the scientists with a handful of questions, including: Since many coronaviruses are relatively harmless, what makes SARS-CoV-2 so damaging to the lungs? What is the appropriate vaccine target for SARS-CoV-2 and in what time framefrom validation to FDA approvalis a vaccine likely? Can we look to drug targets where vaccines have been developed for related viruses and would that timeline be the same? Is there any evidence that SARS-CoV-2 has a mutation rate that is extraordinarily high?

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Could COVID-19 Have Come From Chinese Lab? 4 Things to Know – Daily Signal

Posted: April 12, 2020 at 6:42 pm

Once dismissed as a conspiracy theory, the notion that the new coronavirus spread from a research lab in Wuhan, China has gained more mainstream backing in academia, the media, and at least one government.

There is a credible alternative view based on the nature of the virus, a senior British government official told The Daily Mail. Perhaps it is no coincidence that there is that laboratory in Wuhan. It is not discounted.

Authorities around the world say Wuhan, the capital of Chinas Hubei province, is where the new coronavirus that causes the disease COVID-19 originated.

A South China University of Technology report from February says: the killer coronavirus probably originated from a laboratory in Wuhan.

David Ignatius, a liberal columnist with The Washington Post, presented the plausibility that a lab worker spread the virus; other media outlets quoted medical experts affirming the same possibility.

Still, there is much skepticism.

Wuhan has 11 million people. Its likely they have several labs experimenting on animals and researching diseases, Dean Cheng, senior research fellow for Asian studies at The Heritage Foundation, told The Daily Signal. We [the United States] have labs across the country working on various diseases.

Cheng also contended that Chinas own conduct of message shifting, denial, and blame only helped the spread of rumors and conspiracy theories as well as the coronavirus.

China started by saying, Nothing to say here, and pushed implausible statements, Cheng said.

After a tragic occurrence, humans have a tendency to search for conspiracy theories, said Thomas Spoehr, a retired Army lieutenant general who directs the Center for National Defense at The Heritage Foundation.

We should look for the simplest explanation, and believing this escaped from a lab is not the simplest explanation, Spoehr told The Daily Signal. Like other forms of influenza, this mutated into something unfortunate.

Spoehr said Chinese government officials did invite suspicion with their actions.

Like a witness at a trial that has a reputation for not being truthful and honest with facts, a jury is not likely to believe them, Spoehr said. In this case, people are more likely to assume the worst about China and believe [the new coronavirus] started in a lab.

Here are four things to know about the Wuhan lab theory.

First, what its almost certainly not.

Although early internet gossip pushed the coronavirus as a biological weapon engineered in a Chinese lab, many experts say there is no credible evidence of that.

A study in mid-March by Scripps Research, published by the journal Nature Medicine, strongly states that COVID-19also referred to as SARS-CoV-2follows the natural process similar to related strains of coronavirus.

This evidence for natural evolution was supported by data on SARS-CoV-2s backboneits overall molecular structure, a statement from Scripps Research says, adding:

If someone were seeking to engineer a new coronavirus as a pathogen, they would have constructed it from the backbone of a virus known to cause illness. But the scientists found that the SARS-CoV-2 backbone differed substantially from those of already known coronaviruses and mostly resembled related viruses found in bats and pangolins.

A leading expert on bioweapons is definitive on the matter.

There is no evidence whatsoever that this is a bioweapon or that it was accidentally released from the Wuhan lab, said Michael Osterholm, director of the Center for Infectious Disease Research Policy at the University of Minnesota and author of Living Terrors: What Our Country Needs to Survive the Coming Bioterrorist Catastrophe.

Today, with the genetics we have on these viruses and how we can do testing, we can almost date them almost like carbon testing so radiocarbon and you want to know how old a block is or something like that, Osterholm said during an interview in March on The Joe Rogan Experience podcast.

Osterholm said the new coronavirus clearly jumped from an animal species, probably the third week of November to humans.

I dont believe that theres any evidence linking this to an intentional release or an accidental release, or that its an engineered bug. Its not, he said.

But the theory that the coronavirus came from an animal does not entirely back up the prevailing wisdom that the virus originated in a wet market in Wuhan, according to recent studies.

About 1.5 million cases of COVID-19 have been confirmed around the world, with 100,000 deaths. The U.S. government has projected at least 100,000 deaths in America before the pandemic passes.

But far deadlier pathogens exist. If the Chinese wanted to develop a biological weapon, Cheng said, its likely its communist government would have aimed for something far more lethal than COVID-19.

The prevailing wisdom that the virus was spread by people who ate contaminated animals at the Huanan Seafood Market in Wuhan, Ignatius wrote in the Post, is shaky.

He noted that bats werent sold at the seafood market, although that market or others could have sold animals that had contact with bats.

Wuhan authorities closed that seafood market and disinfected it without swabbing individual animals and cages, or drawing blood from workers, according to The New York Times.

It is absolutely clear the market had no connection with the origin of the outbreak virus, and, instead, only was involved in amplification of an outbreak that had started elsewhere in Wuhan almost a full month earlier, Richard Ebright, a Rutgers University professor of chemistry and chemical biology, told CNN.

A study published Jan. 24 found that the early coronavirus cases were not connected to the Huanan Seafood Market in Wuhan. The study was published in February by The Lancet, a weekly peer-reviewed medical journal.

The study found that the first case was reported Dec. 1, 2019 by an elderly man who had no contact with the Huanan Seafood Market.

One of the reports co-authors, Wu Wenjuan, a senior doctor at Wuhans Jinyintan Hospital, reportedly said the man had Alzheimers disease, lived four or five buses from the seafood market, and because he was sick he basically didnt go out.

Research labs exist close to the Huanan Seafood Market in Wuhan, prompting what Ignatius referred to as a competing theory.

Less than 300 yards from the seafood market is the Wuhan branch of the Chinese Center for Disease Control and Prevention, Ignatius wrote in the Post:

Researchers from that facility and the nearby Wuhan Institute of Virology have posted articles about collecting bat coronaviruses from around China, for study to prevent future illness. Did one of those samples leak, or was hazardous waste deposited in a place where it could spread?

COVID-19 also could have occurred as a laboratory accident, with, for example, an accidental infection of a laboratory worker, Ebright, of Rutgers University, told the Post columnist.

Coronaviruses in bats were being studied in Wuhan only at Biosafety Level 2, which provides only minimal protection, Ebright said. Biosafety Level 4 is the highest level of security.

In a December video from the Chinese Center for Disease Control and Prevention lab, staffers are seen collecting bat coronaviruses with inadequate [personal protective equipment] and unsafe operational practices, the Rutgers microbiologist is quoted as saying in the Post.

Cheng noted that COVID-19 is a new strain of coronavirus, and that Chinese researchers likely didnt anticipate the need for the highest security level.

We could require a SWAT team every time you make a police call just as you could always have maximum security in labs, but it would become very costly, Cheng said.

In the interview with CNN, Ebright said, The possibility that the virus entered humans through a laboratory accident cannot and should not be dismissed.

Ebright stressed that there were no signatures of human manipulation, but said a lab worker might have contracted the disease and accidentally spread it.

Chinese news articles from 2017 and 2019 described in heroic terms how Wuhan CDC researcher Tian Junhua, capturing bats in a cave, forgot to take protective measures and that bat urine dripped from the top of his head like raindrops, Ignatius wrote, quoting the articles.

ResearchGate published the South China University of Technology study, which said: It is plausible that the virus leaked around and some of them [lab workers] contaminated the initial patients in this epidemic, though solid proofs are needed in future study.

The study concluded:

In summary, somebody was entangled with the evolution of 2019-nCoV coronavirus. In addition to origins of natural recombination and intermediate host, the killer coronavirus probably originated from a laboratory in Wuhan. Safety level may need to be reinforced in high risk biohazardous laboratories. Regulations may be taken to relocate these laboratories far away from city center[s] and other densely populated places.

One of the Wuhan CDC researchers quarantined himself after being exposed to bat blood, and also found a tick on the bat that could have spread the disease, according to the university report.

At a time when China was suppressing information on COVID-19, the authors withdrew the report.

The Wall Street Journal reported in February that co-author Botao Xiao said he had withdrawn the paper because, The speculation about the possible origins in the post was based on published papers and media, and was not supported by direct proofs.

Aside from any evidence about starting in a labanecdotal or otherwiseChinas early response to the coronavirus may have been a reason that suspicion spread more quickly than the disease itself.

The misinformation from delayed what would have been an early response from the international community. Later, mid-level Chinese government officials accused the U.S. Army of planting the virus in Wuhan.

Sen. Tom Cotton, R-Ark., was one of the earliest members of Congress to raise alarm about the coronavirus.

Cottons office wasnt ready to weigh in on the origins of the virus or whether Congress would investigate. But the senator said China is responsible for spreading misinformation about COVID-19.

China must be held accountable for unleashing this plague on the world, Cotton told The Daily Signal in a written statement, noting he has introduced legislation to do just that.

In early January, eight Chinese doctorsincluding Dr. Li Wenliangwarned about the coronavirus. The government brought them in for questioning and condemned them for making false statements.

Also in early January, Chinas National Health Commission directed that COVID-19 samples from Wuhan be removed and destroyed, Caixin Global reported.

Also that month, the Wuhan Municipal Health Commission stopped releasing daily updates of new COVID-19cases, The Wall Street Journal reported.

Government officials and state-run media later shifted the message, raising questions as to whether the virus originated in China or elsewhere.

The World Health Organization parroted many of the Chinese propaganda points in its slow response to the disease. This prompted a likely congressional investigation of WHO.

Meanwhile, the Trump administration is considering defunding WHO for its botched response to the original outbreak.

Asked Wednesday at a press briefing whether China withheld critical information, Secretary of State Mike Pompeo avoided a direct answer.

You know, this is not the time for retribution, but it is still the time for clarity and transparency, Pompeo said, adding:

Were still working on this problem set. Theres still data that these good people need so that they can perform their analysis of how to both develop therapeutics and a vaccine and to understand where this virus is. So, every country, China included, every country needs to be transparent about whats gone on in their country.

But Pompeo added of the virus: It started in China, and so they had that special responsibility to get it right quickly and fast.

After a tragic occurrence, there is a tendency to search for conspiracy theories, said Thomas Spoehr a former former Army lieutenant general and now the director of Center for National Defense at The Heritage Foundation.

We should look for the simplest explanation and believing this escaped from a lab is not the simplest explanation, Spoehr told The Daily Signal. Like other forms of influenza, this mutated into something unfortunate.

Spoehr said China did invite the suspicion.

Like a witness at a trial that has a reputation for not being truthful and honest with facts, a jury is not likely to believe them, Spoehr said. In this case, people are more likely to assume the worst about China and believe it started in a lab.

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Testosterone Replacement Therapy Market Growth Analysis, Top Manufacturers, Shares, Growth Opportunities and Forecast to 2026 – Germany English News

Posted: April 12, 2020 at 6:41 pm

New Jersey, United States:The new report has been added by Market Research Intellect to provide a detailed overview of the Testosterone Replacement Therapy Market. The study will help to better understand the Testosterone Replacement Therapy industry competitors, the sales channel, Testosterone Replacement Therapy growth potential, potentially disruptive trends, Testosterone Replacement Therapy industry product innovations and the value / volume of size market (regional / national level, Testosterone Replacement Therapy- Industrial segments), market share of the best actors / products.

Information has been added to the report to provide a realistic view of the industry based on data from Testosterone Replacement Therapy manufacturers, i.e. H. Shipping, price, sales, gross profit, business distribution, etc., SWOT analysis, consumer preference, current developments and trends, drivers and limiting factors, company profile, investment opportunities, analysis of the demand gap, market size value / volume, services and products, Porters five models , socio-economic factors, official regulations in the Testosterone Replacement Therapy branch. Market participants can use the report to take a look at the future of the Testosterone Replacement Therapy market and make significant changes to their operating style and marketing tactics in order to achieve sustainable growth.

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The report examines the competitive environment scenario observed with key players in Testosterone Replacement Therapy sales, the profile of their business, their earnings, their sales, their business tactics, and the forecasting situations of the Testosterone Replacement Therapy sales industry. According to studies, the Testosterone Replacement Therapy sales market is very competitive and diverse due to global and local suppliers.

The Testosterone Replacement Therapy Sales Market Report mainly contains the following Manufacturers:

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The competitive landscape of the Testosterone Replacement Therapy market is examined in detail in the report, with a focus on the latest developments, the future plans of the main players and the most important growth strategies that they have adopted. The analysts who compiled the report have created a portrait of almost all of the major players in the Testosterone Replacement Therapy market, highlighting their key commercial aspects such as production, areas of activity and product portfolio. All companies analyzed in the report are examined on the basis of important factors such as market share, market growth, company size, production, sales and earnings.

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Market entry analysis

market size and forecast

The geographic department provides data that give you an overview of the turnover of companies and sales figures for the growth activity Testosterone Replacement Therapy for electrical meters. Here are the strengths of the geographic divisions: North America (United States, Canada and Mexico), Europe (Germany, Spain, France, Great Britain, Russia and Italy and more), Asia-Pacific (China, Japan, Korea, India and Southeast Asia) and more ), South America (Brazil, Argentina, Colombia), the Middle East and Africa (Saudi Arabia, United Arab Emirates, Egypt, Nigeria and South Africa) and ROW.

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Table of Content

1 Introduction of Testosterone Replacement Therapy Market1.1 Overview of the Market1.2 Scope of Report1.3 Assumptions

2 Executive Summary

3 Research Methodology3.1 Data Mining3.2 Validation3.3 Primary Interviews3.4 List of Data Sources

4 Testosterone Replacement Therapy Market Outlook4.1 Overview4.2 Market Dynamics4.2.1 Drivers4.2.2 Restraints4.2.3 Opportunities4.3 Porters Five Force Model4.4 Value Chain Analysis

5 Testosterone Replacement Therapy Market, By Deployment Model5.1 Overview

6 Testosterone Replacement Therapy Market, By Solution6.1 Overview

7 Testosterone Replacement Therapy Market, By Vertical7.1 Overview

8 Testosterone Replacement Therapy Market, By Geography8.1 Overview8.2 North America8.2.1 U.S.8.2.2 Canada8.2.3 Mexico8.3 Europe8.3.1 Germany8.3.2 U.K.8.3.3 France8.3.4 Rest of Europe8.4 Asia Pacific8.4.1 China8.4.2 Japan8.4.3 India8.4.4 Rest of Asia Pacific8.5 Rest of the World8.5.1 Latin America8.5.2 Middle East

9 Testosterone Replacement Therapy Market Competitive Landscape9.1 Overview9.2 Company Market Ranking9.3 Key Development Strategies

10 Company Profiles10.1.1 Overview10.1.2 Financial Performance10.1.3 Product Outlook10.1.4 Key Developments

11 Appendix11.1 Related Research

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Testosterone Replacement Therapy Market Growth Analysis, Top Manufacturers, Shares, Growth Opportunities and Forecast to 2026 - Germany English News

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Another year in isolation? That’s great news for one San Diego family – The San Diego Union-Tribune

Posted: April 11, 2020 at 8:44 pm

On a recent grocery run, Katie Luckesen noticed something new: she wasnt the only shopper wearing a face mask and gloves.

And this time, she added, I didnt get any funny looks!

If anyone was ready for the current era of social distancing and hyper-cleanliness, it was the Luckesens. For more than two years, this South San Diego family has lived in lockdown to protect their youngest member.

Charlie Luckesen was born in November 2017 without a thymus, the gland that transforms stem cells into T cells able to fight infections and disease. Lacking this essential piece of the immune system, the child could be killed by even a minor illness.

This is a rare condition: athymia afflicts only five out of every 1 million babies born in the U.S. Theres only one cure, a transplant, and only one place in the U.S. that performs this operation, Duke University.

On March 10, Charlie became that North Carolina medical centers 102nd thymus tissue transplant. Hes home now, yet the Luckesens will self-quarantine until the new organ produces a full set of robust T cells. That may take up to a year.

Another 12 months in lockdown? To Charlies mom, that prospect is thrilling.

I finally feel like I can see the light at the end of the tunnel, she said.

Faith has played a major role in Charlies story. It has sustained Katie Luckesen and her husband, Lt. J.D. Luckesen, a Navy chaplain. It has steeled them for the possibility of losing their son. And it has reminded them on this Easter Sunday that with faith, miracles can happen.

Katie Luckesen said the past two years have been a trial and a test of her faith. She couldnt have gotten through it without what she calls a lively hope, a line from Chapter 1, Verse 3, of the Bibles First Epistle of Peter, about the miraculous resurrection of Jesus Christ on Easter morning.

Easter is a reminder to myself that even if Charlie would die that it wasnt the end, she said. I praise God for the knowledge of the resurrection that reminded me that mortal separations are temporary, and this gave me the hope and strength to carry on when days were hard.

The atonement of Christ becomes even more personal this Easter as I think that he suffered so that he could succor me in all of my trials, she said. I have come to realize the power and peace of the praying for courage to have the strength to say not my will, but thine be done ... We didnt come this far, only to come this far.

For most of his young life, Charlie had been on Dukes transplant waiting list. These were tense times for the Luckesens who, through social media, shared their trials and triumphs with other families engaged in the same struggle.

All pray for a transplant, but not all of these prayers are answered. The average lifespan of someone born without a thymus is two years. Last November, on Charlies second birthday, one of these children died. A week later, another followed.

I feel like we are living on borrowed time, Luckesen said then.

Complicating matters was a Food and Drug Administration ruling in December, withholding approval of RVT-802, a patented process used to culture thymus tissue and prepare it for transplantation. In the end, Duke won the FDAs permission to continue using the method under a research protocol.

Finally, the day came. On Feb. 17, Charlie had a quick checkup with his pediatrician, Dr. Katie Lively Swartz of Balboa Naval Medical Center Just to make sure things went smoothly, the doctor said. Then the boy and his mother boarded an air ambulance for the cross-country flight to Dukes campus in Durham, N.C.

There, Charlie underwent a battery of tests and was treated with pre-transplant medications.

He had a strong reaction to that, a big seizure, Luckesen said. He was able to power through it.

The toddler had recovered when a fever racked his body. Worried that this might indicate a serious ailment, doctors at Duke ordered a full body CT scan.

This was the worlds most expensive sinus infection diagnosis, she said.

On March 10, Charlie was wheeled into an operating room in Duke. The long-awaited procedure took a mere three hours.

Its a really minor, anti-climatic surgery for how monumental it is in our lives, Luckesen said. They put tiny pieces of thymus up and down his leg.

Dr. M. Louise Markert, a Duke professor of pediatrics and immunology, pioneered this technique in 1993, inspired by infants who undergo heart surgery.

At birth, the thymus is located over the heart. When heart surgeons operate on infants, they often need to remove some thymus tissue to gain access to the heart.

The material that is cut away can be used in a thymus tissue transplant, but only when parents agree to literally donate part of their own child to strangers.

Katie Luckesen holds infant Charlie in the NICU at Rady Childrens Hospital in San Diego in May 2018.

(Nelvin C. Cepeda / The San Diego Union-Tribune)

This is a wonderful story of parents who, and you can imagine the stress they are under, are able to think about someone else, Markert said. The only way this can be done is through the goodness of peoples hearts.

Donated tissue, which is tested to ensure its compatibility to the recipient, is usually implanted in the thigh muscle. In Charlies case, about 40 pieces of thymus tissue were stitched into his left thigh.

It went according to plan, Markert said of Charlies operation. I think this one went very smoothly.

When Charlie and his mother arrived in North Carolina in February, the coronavirus was just beginning to disrupt life in the U.S. A month later, with Charlie recovering at Duke, Luckesen worried the pandemic would delay their trip home.

In fact, an air ambulance flew mother and child back to San Diego on March 26. Charlies long-term prospects are bright the survival rate for thymus tissue transplant recipients is 72 percent, which includes patients with other health issues.

Deaths, Markert said, are rare once a transplant patient survives the first year or two.

The Luckesens will remain in isolation as Charlies newly-acquired thymus tissue equips him with functioning T cells. This may mean another year of stringent precautions. Charlies father is remaining at his post at the Marine Corps Recruit Depot, rather than risk infecting his wife and children.

Were treating this like a deployment, she said.

This adds another layer of stress on the entire family, she acknowledged. But this is the best we can protect Charlie.

Charlie,2 and his mother, Katie Luckesen recently returned from North Carolina where Charlie underwent Thymus transplant procedure at Duke University Hospital.

(Nelvin C. Cepeda/Nelvin C. Cepeda/The San Diego Union-Tribune)

While most Americans are new to the demands of self-quarantining, the Luckesens have been there, done that and disinfected it all.

Were pros, Luckesen said.

Charlie was nearly 9 months old before he left his sterile room at Rady Childrens Hospital. Once the baby came home, his siblings Jack, who is now 8; William, 6; and Amelia, 5 were taken out of school. Classes are now held at home.

Visitors are rarely allowed inside, and only after slipping on hospital gloves, surgical masks, gowns and booties. These supplies are stored by the front door, alongside a stockpile of hand sanitizers and disinfectants.

Charlie Luckesen, 2, enjoyed playing with his three siblings, William, 6; Jack, 8; and Amelia, 5, during their playtime on the front yard of the familys home.

(Nelvin C. Cepeda/The San Diego Union-Tribune)

A Roomba roams the downstairs, vacuuming up stray grit, while Luckesen constantly washes, wipes, cleans.

Despite the occupation of Charlies father, the family stopped attending church services in person long ago, turning to internet services in order to avoid germs.

The children play in the backyard and on the front lawn, but only with each other. They walk in the neighborhood, but avoid other pedestrians and all crowds.

Given her hard-won expertise, Luckesen is sometimes asked how to handle social distancing and other protective measures being adopted in the coronavirus era.

My biggest advice, she said, is just to be diligent.

For this family, that means wearing gloves and face masks on any journey outside the home. On their return, they shower and change into fresh clothes, dropping the previous outfit into the washing machine.

Theres a lot of hand washing in this household, and lot of forgiveness.

You have to give yourself grace, to let things slide that dont matter as much, Luckesen said. My kids probably have too much screen time, probably watch more TV than they should. But I have to keep the house clean for Charlie.

Charlie was slow to develop, held back by his condition and his treatments. (One example: his frequent need for diuretics hindered his absorption of calcium, leading to a fractured vertebrae.)

When he came home from Duke, though, he seemed eager to make up for lost time.

Hes doing great, Luckesen said. Hes pulling himself up and he loves to take steps and walk with me, holding on.

Charlie will need close monitoring, and the family needs to live in isolation, for some time. Every week, the toddlers blood samples will be checked by physicians here. Every month, his T cells will be surveyed by specialists at Duke.

But I finally think we are starting to see the finish line of a marathon, she said.

Life is changing, in ways that allow the Luckesens to hope.

This will be the first year he wont be in the hospital for his birthday, Luckesen said of her youngest child. Nobody wants to be in the hospital for their birthday.

Today the family will celebrate Easter not only separated, with J.D. stationed on base at MCRD, but without a traditional church service. But Luckesen said she plans to connect with her family over FaceTime today and share her faith with others.

We can come together in our families at home. We can celebrate Easter without having to be in a building. We can come together through technology and worship through one of the many online church services. We can come together in reaching out virtually or over the phone with our relatives, she said.

For me the celebration of Easter means rejoicing in the resurrection of Jesus Christ and sharing my witness and testimony.

Staff writer Pam Kragen contributed to this report.

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Another year in isolation? That's great news for one San Diego family - The San Diego Union-Tribune

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Man hospitalized with COVID-19 learns he also has diabetes. Why that’s dangerous. – NBC News

Posted: April 11, 2020 at 8:44 pm

Rico Ramirez spent 10 days in a San Francisco hospital's COVID-19 unit, hooked up to oxygen to help him breathe, isolated from family and friends.

"I thought I was going to die alone," Ramirez told NBC affiliate KNTV. "I thought every day I was in there that I was going to die in a room by myself."

Full coverage of the coronavirus outbreak

But coronavirus wasn't the only illness he learned about when he was hospitalized; he also learned he has Type 2 diabetes, putting him at greater risk for complications from the virus.

"I'm just happy to be alive," Ramirez, who's now in recovery, said.

Ramirez joins the estimated 34 million Americans who have diabetes. The disease, in which the body is unable to produce enough insulin to keep blood sugar levels in check, is often listed as an underlying condition for COVID-19 patients sick enough to be hospitalized and put on a ventilator.

Earlier this week, the Centers for Disease Control and Prevention released findings on 178 hospitalized patients who had other chronic health problems. Nearly a third had diabetes.

Patients with diabetes also often have other underlying conditions, such as obesity and high blood pressure, putting them at even greater risk for coronavirus complications. The CDC report found nearly half of those hospitalized patients also had hypertension and/or obesity.

Diabetes weakens a person's immune system.

"People with diabetes are more prone to infections, and if they have infections, they're more prone to poor outcomes," Dr. John Buse, the head of endocrinology at the UNC School of Medicine in Chapel Hill, North Carolina, said.

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Doctors say this is especially true for those with uncontrolled blood sugar levels.

"Poorly controlled diabetes affects the immune system in various ways," Dr. Mary Vouyiouklis Kellis, an endocrinologist at the Cleveland Clinic, said. When blood glucose levels are too low or too high, it makes it more difficult for the body's army of white blood cells to function effectively, she said.

"That's when the immune system starts to go haywire," Kellis said. "The ability to fight infection is diminished."

Doctors also hypothesize that the chronic, low levels of inflammation associated with Type 2 diabetes may also worsen outcomes of patients with the coronavirus.

Inflammatory reactions in the body are not necessarily a bad thing and happen as a natural response to injury. For example, the swelling seen after twisting your ankle is an inflammatory response. But this type of response differs from the chronic inflammation seen in people with diabetes, because the swollen ankle eventually shrinks back down to size.

If a person's level of inflammation is chronically elevated, even at low levels, an infection such as the coronavirus may prompt the body to release far too many of those inflammatory chemicals, called cytokines.

"It seems that a lot of the bad outcomes with COVID-19 are related to a hyperinflammatory" response, referred to as a cytokine storm, Buse told NBC News.

"Instead of releasing enough cytokines to control the infection, the body almost overdoes it," Kellis said, "and it becomes too much for the body to handle."

Keeping blood sugar levels in check is critical, doctors say.

"That's really the most important thing," Kellis said. "Get your blood sugar as controlled as possible so if you do see elevations, you can make changes to get it back to where you need to be."

And if you do notice an unexplained rise in blood sugar, that may be an early warning sign of illness.

It's like "an antenna that goes up that says something may be coming," Dr. Robert Eckel, president of medicine and science at the American Diabetes Association, said.

Download the NBC News app for full coverage of the coronavirus outbreak

Eckel explained patients may see a rise in blood sugar 12 to 24 hours before developing symptoms, such as a cough or a fever. This is a known phenomenon that doesn't just apply to COVID-19; it's true for people with diabetes when they develop other illnesses, too.

"If you're used to a fairly stable picture of your blood glucose and now you're seeing an escalation," Eckel said, "pay attention to what may be following."

The American Diabetes Association has other suggestions for people with diabetes now that millions are staying home as much as possible:

Beyond those tips, the basics of infection prevention apply: proper hand washing, social distancing and covering your face in public.

Follow NBC HEALTH on Twitter & Facebook.

Erika Edwards is a health and medical news writer and reporter for NBC News and "TODAY."

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Man hospitalized with COVID-19 learns he also has diabetes. Why that's dangerous. - NBC News

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Styling out coronavirus with diabetes and tie-dye – BBC News

Posted: April 11, 2020 at 8:44 pm

Beth and Ellen had been enjoying their moment in the spotlight as the Diabetic Duo when coronavirus came along.

The type 1 diabetics had become known for their videos on the social media platform TikTok and dispelled myths around the condition, but the current pandemic and self-isolation has meant theyve had to get creative with how they make their content.

In this episode of Cabin Fever the duo reveal why diabetes is classed as High Risk in relation to Covid-19, how their emotions affect their blood sugar levels and their recent obsession with tie-dye loungewear.

This week's presenters are having quite different quarantine issues. Simon Minty is a little person and says that had a stranger turned away from him in the street he would have taken it badly a few weeks ago - now it's positively welcome!

And Emma feels liberated by lockdown. As a blind mum she says her house and garden are her castle and being at the home she knows so well means she can run about and play with her young boys independently.

Produced by Beth Rose. A full transcript is available here.

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Styling out coronavirus with diabetes and tie-dye - BBC News

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At the barbershop: a trim and a diabetes screening – Health24

Posted: April 11, 2020 at 8:43 pm

Hundreds of black men recently discovered they could get more than a trim at their local barbershops. They were offered diabetes testing, too.

A new study offered customers diabetes screenings at eight New York City barbershops. Among those who took the test, 10% learned they had average blood sugar levels that indicated type 2 diabetes. And almost 30% appeared to have prediabetes.

"For a long time, barbershops have been a place of trust, especially for black people. Because we had the barbers on board with us, people trusted us. Barbers are often important health advocates," said the study's senior author, Dr David Lee. He's an assistant professor of emergency medicine at the NYU School of Medicine.

Lee and his colleagues said it's important to reach out to black men in this way because their diabetes diagnosis is often delayed, and black men have significantly higher rates of diabetes complications once diagnosed. Black men are also less likely to live into their 70s than are men in other racial and ethnic groups.

Dr Anthony Clarke, an internal medicine doctor in Detroit, said, "Not seeking medical care is a common problem in men, and it's worse in the black community. With a lot of men in general, they think, if you don't know about a problem, it's OK. A lot of men tell me, 'My wife made me come in.'"

Clarke, of Detroit Medical Center's Harper University Hospital, was not involved in the current study.

"I think the barbershop was a good way to do this. If patients aren't coming to you, you go where the patients are. The barbershop is a gathering place for men," Clarke said.

The researchers partnered with eight Brooklyn barbershops, all owned by black people. The neighbourhoods were chosen because they had a higher prevalence of poor blood sugar control.

From September 2017 through January 2019, nearly 900 black men were offered the free finger-stick blood test for diabetes.

The researchers ended up testing 290 men. Their average body mass index (BMI) was 29.3. BMI is a rough estimate of body fat based on height and weight measurements. A BMI between 24.9 and 29.9 is considered overweight. A BMI of 30 or more is considered obese.

Of those who had undiagnosed diabetes, about 62% were obese, the study found. The average age of the men with undiagnosed diabetes was 41.

More than half of the men who didn't take the test were willing to tell the researchers why. About half said they knew their health status or had been checked by their doctor, and 35% said they were healthy or they didn't want to know their status. Eight percent said they were afraid of needles, Lee's team noted.

"The symptoms of type 2 diabetes are relatively few. A lot of people feel fine and think they're healthy. Other research has shown that if you find diabetes from a screening test rather than symptoms, you'll have half the premature mortality rate than those who find out later," Lee said.

"I usually meet people late in the disease process at the [emergency department]. We need to start figuring out ways to detect chronic diseases like diabetes earlier," he added.

Lee said he's not sure if this approach would work in other cities or in more rural areas.

The study results were published as a letter in the online edition of JAMA Internal Medicine.

Image credit: iStock

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