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Dr. Kenneth Pettine Announces Verification of Clinical Safety Trial – Yahoo Finance

Posted: February 3, 2020 at 1:41 pm

Kenneth Pettine's stem cell product to treat OA was tested on retired Navy SEALs

FORT COLLINS, CO / ACCESSWIRE / February 3, 2020 / Kenneth Pettine is proud to announce that his revolutionary mesenchymal stem cell product to treat osteoarthritis was recently tested on 33 former Navy SEALs (one is a medal of honor recipient).

Kenneth Pettine is co-founder of Paisley Laboratories and a co-developer of a bone marrow-derived mesenchymal stem cell active growth factor and exosome product that is anticipated to revolutionize regenerative medicine.

In this study, Extracellular Vesicle Isolate Product (EVIP) was injected into 33 retired Navy SEALs to assist with knee, shoulder, elbow, ankle, and wrist osteoarthritis. At three-month follow-up, the injection appeared both safe and effective, with improvements ranging from 40% to as high as 98%. The average improvement is over 70%.

"This is extremely promising and we are motivated to continue our clinical studies to improve the quality of life for patients," says Kenneth Pettine.

Kenneth Pettine notes in his study that over 50 million Americans require daily treatment for disability and pain associated with OA. Every year, over one million total hip and knee replacements are performed in the U.S. with direct costs of over $30 billion and indirect costs of over $200 billion, with these numbers expected to double in the next three years.

In addition to this trial, Kenneth Pettine has three additional clinical studies planned to evaluate his stem cell products to treat erectile dysfunction, chronic obstructive pulmonary disease (COPD), and chronic lower back pain from painful discs.

For more information, visit https://www.kenneth-pettine.com/

About Kenneth Pettine

Dr. Kenneth Pettine is a serial entrepreneur and published clinical researcher with over 30 years of experience as an orthopedic surgeon. He holds a medical degree from the University of Colorado School of Medicine and completed his master's degree in orthopedic surgery and residency at the Mayo Clinic in Rochester, Minnesota.

In 1991, Dr. Pettine founded the Rocky Mountain Associates in Orthopedic Medicine. Kenneth Pettine is also the founder of Paisley Laboratories and the co-founder of the Society for Ambulatory Spine Surgery. In addition, he co-invented the Prestige cervical artificial disc and the Maverick Artificial Disc. Dr. Pettine is the principal investigator of 18 FDA studies involving non-fusion implants, biologics, and stem cells. He holds the only two issued U.S. patents for performing stem cell joint and spinal injections and currently has 21 additional patents pending for bone marrow derived mesenchymal stem cell applications. Kenneth Pettine is also a philanthropist and currently has a scholarship program underway to help students fund their education.

For more information, visit https://www.kenneth-pettine.com/ or https://www.kennethpettinescholarship.com/

Contact

info@kenneth-pettine.com

https://www.kenneth-pettine.com/

SOURCE: Kenneth Pettine

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Dr. Kenneth Pettine Announces Verification of Clinical Safety Trial - Yahoo Finance

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Snake venom can now be made in a lab and that could save many lives – CNN

Posted: February 3, 2020 at 1:41 pm

It involves milking snake venom by hand and injecting it into horses or other animals in small doses to evoke an immune response. The animal's blood is drawn and purified to obtain antibodies that act against the venom.

Producing antivenom in this way can get messy, not to mention dangerous. The process is error prone, laborious and the finished serum can result in serious side effects.

Experts have long called for better ways to treat snake bites, which kill some 200 people a day.

Now -- finally -- scientists are applying stem cell research and genome mapping to this long-ignored field of research. They hope it will bring antivenom production into the 21st Century and ultimately save thousands, if not hundreds of thousands, of lives each year.

Researchers in the Netherlands have created venom-producing glands from the Cape Coral Snake and eight other snake species in the lab, using stem cells. The toxins produced by the miniature 3-D replicas of snake glands are all but identical to the snake's venom, the team announced Thursday.

"They've really moved the game on," said Nick Cammack, head of the snakebite team at UK medical research charity Wellcome. "These are massive developments because it's bringing 2020 science into a field that's been neglected."

Hans Clevers, the principal investigator at the Hubrecht Institute for Developmental Biology and Stem Cell Research in Utrecht, never expected to be using his lab to make snake venom.

So why did he decide to culture a snake venom gland?

Clevers said it was essentially a whim of three PhD students working in his lab who'd grown bored of reproducing mouse and human kidneys, livers and guts. "I think they sat down and asked themselves what is the most iconic animal we can culture? Not human or mouse. They said it's got to be the snake. The snake venom gland."

"They assumed that snakes would have stem cells the same way mice and humans have stems cells but nobody had ever investigated this," said Clevers.

After sourcing some fertilized snake eggs from a dealer, the researchers found they were able to take a tiny chunk of snake tissue, containing stem cells, and nurture it in a dish with the same growth factor they used for human organoids -- albeit at a lower temperature -- to create the venom glands. And they found that these snake organoids -- tiny balls just one millimeter wide -- produced the same toxins as the snake venom.

The team compared their lab-made venom with the real thing at the genetic level and in terms of function, finding that muscle cells stopped firing when exposed to their synthetic venom.

The current antivenoms available to us, produced in horses not humans, trigger relatively high rates of adverse reactions, which can be mild, like rash and itch, or more serious, like anaphylaxis. It's also expensive stuff. Wellcome estimate that one vial of antivenom costs $160, and a full course usually requires multiple vials.

Even if the people who need it can afford it -- most snakebite victims live in rural Asia and Africa -- the world has less than half of the antivenom stock it needs, according to Wellcome. Plus antivenoms have been developed for only around 60% of the world's venomous snakes.

In this context, the new research could have far-reaching consequences, allowing scientists to create a biobank of snake gland organoids from the 600 or so venomous snake species that could be used to produce limitless amounts of snake venom in a lab, said Clevers.

"The next step is to take all that knowledge and start investigating new antivenoms that take a more molecular approach," said Clevers.

To create an antivenom, genetic information and organoid technology could be used to make the specific venom components that cause the most harm -- and from them produce monoclonal antibodies, which mimic the body's immune system, to fight the venom, a method already used in immunotherapy treatments for cancer and other diseases.

"It's a great new way to work with venom in terms of developing new treatments and developing antivenom. Snakes are very difficult to look after," Cammack said, who was not involved with the research.

Clevers said his lab now plans to make venom gland organoids from the world's 50 most venomous animals and they will share this biobank with researchers worldwide. At the moment, Clevers said they are able to produce the organoids at a rate of one a week.

But producing antivenom is not an area that pharmaceutical companies have traditionally been keen to invest in, Clevers said

Campaigners often describe snakebites as a hidden health crisis, with snakebites killing more people than prostrate cancer and cholera worldwide, Cammack said.

"There's no money in the countries that suffer. Don't underestimate how many people die. Sharks kill about 20 per year. Snakes kill 100,000 or 150,000," said Clevers.

"I'm a cancer researcher essentially and I am appalled by the difference in investment in cancer research and this research."

One challenge to making synthetic antivenom is the sheer complexity of how a snake disables its prey. Its venom contains several different components that have different effects.

Researchers in India have sequenced the genome of the Indian Cobra, in an attempt to decode the venom.

"It's the first time a very medically important snake has been mapped in such detail," said Somasekar Seshagiri, president of SciGenom Research Foundation, a nonprofit research center in India.

"It creates the blueprint of the snake and helps us get the information from the venom glands." Next, his team will map the genomes of the saw-scaled viper, the common krait and the Russell's viper -- the rest of India's "big four." This could help make antivenom from the glands as it will be easier to identify the right proteins.

In tandem, both breakthroughs will also make it easier to discover whether some of the potent molecules contained in snake venom are themselves worth prospecting as drugs -- allowing snakes to make their mark on human health in a different way to how nature intended -- by saving lives.

"As well as being scary, venom is amazingly useful," Seshagari said.

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Chinese New Year babys B.C. family gives gift of life in cord-blood donation – The Province

Posted: February 3, 2020 at 1:41 pm

Jack Chieh and Yinnie Wong with their baby boy, born last Friday (Chinese New Year). The couple donate her baby's cord blood to the cord blood bank at B.C. Womens Hospital & Health Centre.Handout

Yinnie Wong and Jack Chiehs six-pound, 13-ounce baby boy as yet unnamed was born on an auspicious day, Jan. 24, Chinese New Year, and hes already doing good in the world.

Everyone was really happy, it is supposed to be a lucky day, said Wong.

Although the birth was a planned C-section, Wong had no control over the date hospital administrators chose for the birth. What she did have control over was the choice to donate her babys cord blood to the cord blood bank at B.C. Womens Hospital & Health Centre, which has just celebrated its fifth anniversary.

Cord blood is blood that is taken from the umbilical cord and placenta immediately after the birth of a healthy infant. Cord blood is rich in stem cells, and can be used to treat over 80 diseases, including leukemia.

According to Canadian Blood Services, ethnically diverse donors are especially needed because although Stats Canada data shows 67.7 per cent of Canadians consider their ethnic origin to be diverse, only 31 per cent of Canadians with blood in Canadas stem-cell registry are from ethnically diverse backgrounds.

Crystal Nguyen, 20, is a former B.C. Childrens Hospital patient whose life was saved by a stem-cell transplant from donated cord blood. Nguyen was first diagnosed with acute myeloid leukemia at age 12. After chemo, she went into remission for almost three years. Then the cancer returned. She was told she needed a bone-marrow transplant.

Crystal Nguyen, now 20, was first diagnosed with acute myeloid leukemia at age 12. She found a stem-cell match for a needed bone-marrow transplant through the international cord blood bank.Handout

When I relapsed I was very confused, it was kind of surreal. The main thing about being told I needed the bone-marrow stem-cell transplant was confusion, fear and anxiety.

Nguyen is of Vietnamese descent and needed a match to survive. No one in her family was a match, nor was there a stem-cell match in the Canadian cord blood bank, but a match was found thanks to the Canadian Blood Services partnerships with 47 international blood banks.

I was told it came through the international cord blood bank from somewhere very far away, said Nguyen, who has been in remission since the transplant.

When she learned the stem-cell transplant had been successful, Nguyen, who is now studying to become a pediatric oncology nurse, said it felt too good to be true.

There was a lot of happiness, joy, excitement. Donating cord blood is such a simple way to save a life.

Although cord blood can be collected and stored for a fee by private companies and reserved for the donor familys use, cord blood donated through Canadian Blood Services is available free to the public whoever needs the match.

Wong didnt hesitate when her son was born. I felt like I wanted to do it if it helps someone in the public, and if it could save lives I would have been very happy to help another child, said Wong, who is a nurse at B.C. Womens hospital.

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Chinese New Year babys B.C. family gives gift of life in cord-blood donation - The Province

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Heart Muscle Cells Made in the Lab Successfully Transplanted into Patient – Interesting Engineering

Posted: February 3, 2020 at 1:41 pm

A team of researchers at Osaka University in Japan successfully transplanted cardiac muscle cells created from iPS into a patient, who is now recovering in the general ward of the hospital.

The team, led by Yoshiki Sawa, a professor in the university's cardiovascular surgery unit, created the cardiac muscle cells from iPS cells in a clinical trial to verify the safety and efficacy of this type of procedure. The researches want to transplant heart muscle cells into ten patients who have serious heart malfunctions because of ischemic cardiomyopathy over a three year period.

RELATED: RESEARCHERS ORGANIZE STEM CELLS BASED ON A COMPUTATIONAL MODEL

Instead of replacing the heart of patients, the researchers developed degradable sheets of heart muscle cells that were placed on the damaged areas of the heart.

To grow the heart muscle cells in the lab, the researchers turned to induced pluripotent stem cells otherwise known as iPS. Researchers are able to take those iPS cells and make them into any cell they want. In this case, it was heart muscle cells.If the clinical trials prove successful it could remove someday the need for heart transplants.

I hope that (the transplant) will become a medical technology that will save as many people as possible, as Ive seen many lives that I couldnt save, Sawa was quoted at a news conference reported the Japan Times.

As for the patient, the team plans to monitor him during the next year to ascertain how the heart muscle cells perform. According to the Japan Times, the researchers opted to conduct a clinical trial instead of a clinical study because they want approval from Japan's health ministry for clinical application as soon as possible.

The report noted that during the trial the researchers will look at risks, probabilities of cancer and the efficacy of transplanting 100 million cells for each patient that could include tumor cells.

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Efficacy and Safety of Sonidegib in Adult Patients with Nevoid Basal C | CCID – Dove Medical Press

Posted: February 3, 2020 at 1:41 pm

John T Lear,1 Axel Hauschild,2 Eggert Stockfleth,3 Nicholas Squittieri,4 Nicole Basset-Seguin,5 Reinhard Dummer6

1Manchester Royal Infirmary, Manchester, UK; 2Klinik Fr Dermatologie, Venerologie Und Allergologie Universittsklinikum Schleswig-Holstein, Kiel, Germany; 3Universittshautklinik Bochum, Bochum, Germany; 4Sun Pharmaceutical Industries, Inc., Princeton, NJ, USA; 5Department of Dermatology, Hpital Saint Louis, Paris, France; 6Skin Cancer Center University Hospital, Zrich, Switzerland

Correspondence: John T LearUniversity of Manchester, 46 Grafton Street, Manchester M13 9NT, UKTel +44 161 276 4173Fax +44 161 276 8881Email john.lear@srft.nhs.uk

Nevoid basal cell carcinoma syndrome (NBCCS), or Gorlin syndrome, is a rare hereditary disease characterized by the development of multiple cutaneous basal cell carcinomas (BCCs) from a young age.1 Loss-of-function germline mutations in the hedgehog-related patched 1 (PTCH1) tumor suppressor gene are the most common cause of NBCCS.1 The hedgehog signaling pathway plays a major role in embryonic development, and in adulthood, is involved in the renewal and maintenance of distinct tissues, including hair follicles, muscle stem cells, and gastric epithelium.2 Its abnormal activation is thought to drive the formation of both sporadic BCCs and those resulting from NBCCS.1 Patients with NBCCS inherit one inactive copy of PTCH1 and then acquire a second-hit mutation, resulting in hedgehog pathway activation and BCC formation.1 Mutations in Suppressor of fused (SUFU) or the PTCH1 homolog PTCH2 have also been found in a subset of patients meeting criteria for NBCCS.1,3

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License.By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

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Efficacy and Safety of Sonidegib in Adult Patients with Nevoid Basal C | CCID - Dove Medical Press

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Contrasting Mesoblast (NASDAQ:MESO) and Titan Pharmaceuticals (NASDAQ:TTNP) – Riverton Roll

Posted: February 2, 2020 at 2:48 am

Mesoblast (NASDAQ:MESO) and Titan Pharmaceuticals (NASDAQ:TTNP) are both small-cap medical companies, but which is the better stock? We will contrast the two companies based on the strength of their valuation, institutional ownership, risk, earnings, analyst recommendations, dividends and profitability.

Profitability

This table compares Mesoblast and Titan Pharmaceuticals net margins, return on equity and return on assets.

Volatility & Risk

Mesoblast has a beta of 1.82, indicating that its share price is 82% more volatile than the S&P 500. Comparatively, Titan Pharmaceuticals has a beta of 1.18, indicating that its share price is 18% more volatile than the S&P 500.

Analyst Ratings

This is a breakdown of recent ratings for Mesoblast and Titan Pharmaceuticals, as provided by MarketBeat.

Mesoblast currently has a consensus price target of $12.83, indicating a potential upside of 28.21%. Titan Pharmaceuticals has a consensus price target of $1.00, indicating a potential upside of 274.67%. Given Titan Pharmaceuticals stronger consensus rating and higher probable upside, analysts plainly believe Titan Pharmaceuticals is more favorable than Mesoblast.

Insider and Institutional Ownership

2.7% of Mesoblast shares are held by institutional investors. Comparatively, 1.5% of Titan Pharmaceuticals shares are held by institutional investors. 18.8% of Mesoblast shares are held by company insiders. Comparatively, 2.0% of Titan Pharmaceuticals shares are held by company insiders. Strong institutional ownership is an indication that hedge funds, endowments and large money managers believe a company will outperform the market over the long term.

Valuation & Earnings

This table compares Mesoblast and Titan Pharmaceuticals top-line revenue, earnings per share (EPS) and valuation.

Titan Pharmaceuticals has lower revenue, but higher earnings than Mesoblast. Mesoblast is trading at a lower price-to-earnings ratio than Titan Pharmaceuticals, indicating that it is currently the more affordable of the two stocks.

Summary

Mesoblast beats Titan Pharmaceuticals on 9 of the 14 factors compared between the two stocks.

Mesoblast Company Profile

Mesoblast Limited, a biopharmaceutical company, develops and commercializes allogeneic cellular medicines. Its proprietary regenerative medicine technology platform is based on specialized cells known as mesenchymal lineage adult stem cells. The company's products under the Phase III clinical trials include MSC-100-IV for steroid refractory acute graft versus host disease; MPC-150-IM for advanced heart failure; and MPC-06-ID for chronic low back pain due to degenerative disc disease. It is also developing MPC-300-IV that is in Phase II clinical trials for the treatment of biologic refractory rheumatoid arthritis, diabetic kidney diseases, and type 2 diabetic nephropathy. It operates in the United States, Australia, Singapore, the United Kingdom, and Switzerland. Mesoblast Limited has strategic partnerships with Tasly Pharmaceutical Group to offer MPC-150-IM for heart failure and MPC-25-IC for heart attacks in China; and JCR Pharmaceuticals Co. Ltd. for the treatment of wound healing in epidermolysis bullosa. The company was founded in 2004 and is headquartered in Melbourne, Australia.

Titan Pharmaceuticals Company Profile

Titan Pharmaceuticals, Inc., a pharmaceutical company, develops proprietary therapeutics for the treatment of serious medical disorders. It develops products based on ProNeura, a proprietary long-term drug delivery platform that focuses primarily on treatments for chronic diseases. The company offers Probuphine, a product candidate for maintenance treatment of opioid dependence, which maintains a stable, around the clock blood level of the drug buprenorphine in patients for six months following a single treatment. It also develops ProNeura-Ropinirole, an implant to provide delivery of ropinirole, a dopamine agonist for the treatment of Parkinson's disease; and triiodothyronine, an implant for the treatment of hypothyroidism. The company has a collaboration with Nevada Center for Behavioral Health to evaluate a medication-assisted treatment program utilizing Probuphine (buprenorphine) implant for Opioid Use Disorder (OUD) patients. Titan Pharmaceuticals, Inc. was founded in 1992 and is based in South San Francisco, California.

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Contrasting Mesoblast (NASDAQ:MESO) and Titan Pharmaceuticals (NASDAQ:TTNP) - Riverton Roll

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The tree family – Frontline

Posted: February 2, 2020 at 2:46 am

IN the anthropocene, when narratives turn to metaphor to grasp the meaning, deep time and big history, Richard Powers turns to trees in The Overstory. The book, which won the Pulitzer Prize for Fiction in 2019, narrates the stories of nine characters centred around trees as theme, motif and species to configure an account where trees begin to matter.

The novel is the saga of generations and families and the trees that have survived the vagaries of human history. The title refers to the foliage that makes the canopy or the trees that contribute to the overstory and represents distinctive character arcs in the novel. Just like the trees that make up the visible foliage, the nine main characters of the story are a set of people whose lives the author selects to chronicle. The lives of these characters are entangled in serendipitous ways without being envisioned through human tropes such as fate or destiny because The Overstory is not a realistic novel attempting to map human social history. Rather, by having the characters understand trees in different ways such that their paths converge, Powers attempts to map a small cross-section of humans from a larger cosmos of multiple species.

Consider the credentials: Nicholas Hoel is an artist whose family has photographed a chestnut tree every month for generations. Patricia Westerford, the botanist, seems to be modelled after Suzanne Simard, a professor of forest ecology who has put forward theories about communication between trees. She writes a book titled The Secret Forest (probably based on The Hidden Life of Trees, the 2005 bestseller by Peter Wohlleben) and all the main characters read it. Douggie Pavlicek is a Vietnam War veteran who participated in the controversial but real Stanford Prison Experiments as a college student. He falls in love with Mimi Ma, daughter of a Chinese immigrant engineer. We follow the boyhoods of two men: Neelay Mehta, son of Indian immigrants, and Adam Appich, who becomes a psychologist. Ray Brinkman is an intellectual property lawyer, and his wife Dorothy Cazaly is a stenographer. Olivia Vandergriff is an actuary student who has an epiphany after a near-death experience.

Powers background in computers is an important key to the novel. Neelay Mehta, who is confined to his wheelchair, invents a game that will scour databanks for knowledge about trees, so that armed with it we can engage with the natural world to solve our problems. This kind of transhumanist yearning, where corporeal finitude is an obstacle that can be overcome, is only one of the narrative answers to the puzzle that is the tree.

But how does one begin to narrate a tree? There is a happy coincidence between style and subject in The Overstory. Like the expansive trees, Powers is adept at laying out the story in broad brushstrokes. This is not to say that he mimics Charles Dickens in characterisation. His men and women suffer from a lack of detail but the narrative force allows the reader to suspend disbelief and understand them through their encounters and rendezvouses. For example, Neelay Mehta is typecast crudely, with shades of a cleverer Raj Koothrappali, the character in the CBS television series The Big Bang Theory. On the other hand, Patricia is portrayed as an activist like Greta Thunberg or Arundhati Roy. At the same time, reading about Patricia and her difficult childhood offers an invitation to speculate about the life history of an activist academic. What causes the turn from theory to praxis? Is praxis grounded in actions that shape a childhood and is adulthood a reclaiming of that lost agency? This kind of evocative characterisation allows for an openness that the reader is encouraged to inhabit.

The ecological importance of The Overstory lies in its ability to supplant the human in the story. As the network of people branches out across the interlayered stories through chance and connections, the centre appears evanescent, fugacious and non-human. Unlike other anthropocene novels that document the plight of humans in apocalyptic situations, The Overstory is ultimately about trees and how a set of people try to relate to them.

The difficulty of constructing a narrative that can capture the complexity of ongoing ecological catastrophes cannot be overstated. Should it be inspiring and moving? Or should it merely state the facts and expect the readers to draw their own conclusions? Or should the narrative be experimental and try to mirror nature so that form can provoke action? The hidden premise of the novel is the opening line of The Secret Forest that is recounted by several characters: You and the tree in your backyard come from a common ancestor. A billion and a half years ago, the two of you parted ways. It is this common ancestral root that makes the trees come alive in the novel without anthropomorphisation and allegorisation.

It is interesting that this exploration does not require trees to be characters in a rhetorical or Aristotelian sense that would embody a particular quality. Trees are not simply innocent victims out there to be preserved in their pristine virtue. This is made evident in the narrative through a clearly phenomenological investigation that the people engage in through various modes of their lives to acknowledge trees. In Becoming Animal: An Earthly Cosmology, David Abram demonstrates our entanglement with nature by showing how human nature is animal too, and a sensuous awareness can help us realise this dormant essence. Powers does not deny this entanglement of people and trees, but The Overstory upholds a certain distance. People and trees do not belong to the same species. It is this real chasm that the protagonists try to comprehend and decipher.

This is also the source (or shall I say the root?) of the strangeness of the narrative. How do we understand characters or motivations for which trees are not simply trees? The common ancestrality that The Secret Forest professes is what drives the authorial urge to understand trees in the novel. There is an intuition that we and trees belong to the same life process that exceeds our finite understanding because of the brevity of our lifespan and it is this insight that the novel taps into.

Curiously, it is not literature or philosophy that the novel uses to buttress its beliefs but social movements. The novel refers to the Chipko movement in India and the Brazilian Kayapo Indians to understand the concerns that one species may have for another. As the psychology student in the novel remarks, Who does the tree hugger really hug when he hugs a tree? Yet, when people interpose their bodies between tree cutters and the trees, the tree cutters always have their way with violent consequences for the dendrophiles.

Bound up with this cross-species interest toward trees is, of course, the legal suggestion of personhood. If trees acquire legal standing, will plant species cease to become resources worthy only of our consumption and exploitation? Perhaps legal standing will flatten barriers of species and language. The novel grapples with this question through activism. Nick, Olivia, Douglas and Mimi together oppose deforestation and stage protests and sit-ins atop trees. At one point, they even carve out an independent state for themselves called The Bio-Region of Cascadia. But because trees cannot speak back and be grateful or disenchanted, activism only furthers the life stories of the men and women involved, with the trees registering a woody opacity. The only possible acknowledgement remains with the human: will they cut the tree or not?

Against this seeming passivity, trees have a spectral presence in the novel that makes them resemble impenetrable aliens with whom true communication is never possible. Activism, then, is a pledge of the hope that communication, and action based on faith, are possible, despite the diverse personalities of humans and reasons. And as the novel makes clear, perfect harmony with trees or total empathy need not result in satisfying courses of action. There is no cosmic redemption or a promised messiah that will make our sacrifices worthwhile. The descent of environmental activists in the novel after repeated failures to protect the trees from an eco-terrorist group is testimony to this misjudgment.

This is not to say that the different characters do not attempt to anthropomorphise trees or allegorise them. It could be that human communication, even inner speech, is always already humanised. For Dorothy and Ray, the tree symbolises their child. Olivia and Nick give a human characteristic to the redwood tree, Mimas, during their stay atop it. However, here lies the paradox of writing on the environment: by the end of the novel, the reader is not sure whether the anthropomorphisation is inappropriate and the allegorisation insensitive. This is because one is able to see that the perspective of each character towards trees is shaped by their distinctive experiences and personalities, and the reader cannot obviously fault them for that.

The Overstory refrains from making ethical pronouncements for the same reason. While it is accepted that trees need to be protected, the novel never assumes a science fiction/fantasy fiction mode. Thus, though the characters fight for trees in different ways, the novel does not propose a unique or singular solution. In that way, it is certainly not utopic in its aim or hope. It is also not futuristic or idealistic. But as Maidenhair tells Adam, who is there to study them instead of studying people who believe plants are persons, he should study people for whom only other people are real.

The characters, whose lives seem entangled with trees, have chosen to become allies with trees and have, thereby, merely experienced a shift in perspective. Indeed, the main characters, especially Mimi, Douglas, Nick and Olivia, seem to experience time and history differently. Cross-species intimacy allows these characters to take on and dwell in the vital circles of the Umwelt of the trees. Realising that the trees have a longer life process that evokes the cosmic processes of life and cycles, Olivia and Nick live in forest time: They have been on forest time too long to count in mere hours anymore. The work is over in a moment, in the twinkling of an eye. The transience of human life poses an urgency to the matter that the characters are aware of: They cant see that time is one spreading ring wrapped around another, outward and outward until the thinnest skin of Now depends for its being on the enormous mass of everything that has already died. The logo of the cross section of a tree trunk showing the concentric growth rings recurs in the novel to alert us gently to human history as it is being shaped today by deforestation and forest fires.

This intimacy is also a dangerous tendency because at least for some characters it often seems to be on the verge of teetering into a kind of unreflective nativism at whose centre is a tenuous figure such as the ecological Indian. Do we have to discard humanity to reach out to other species? By rejecting civilisation and by extension, technology, what kind of return to the putative innocence of nature do we envision? The utopia certainly cannot be an anarchy of chaos and probably not one of jungles and lush verdure either.

The novel ends with an ambiguous message from Nick: STILL. While a lot of species may not survive the ongoing mass extinction event, there is something to be said about resistance and the possibilities that resistance enfolds, like a seed, of growth, rebirth and change. This still does not answer the question as to whether art is necessary in the anthropocene. While Powers does not privilege art as the mode through which we can understand trees, there is an element of the creative and the artistic at the core of every story in the novel. Thus, Nick is an artist, Ray and Dorothy act in plays, Mimi recites ancient Chinese poetry, Patricia writes a book, Douglas keeps a diary and Olivia assumes a persona, namely, Maidenhair.

Perhaps, it could be said that at the heart of every activism is a flair for the dramatic as well. Of course, even the activists affirm the need for narrative: The best arguments in the world wont change a persons mind. The only thing that can do that is a good story.

The narrative power of The Overstory stems from how the stories of the characters make sense, not from one anothers stories but from the unwritten and unread story of trees. This is possible because of a self-reflexivity that is sewn into the novels texture, which states that it is impossible to narrativise a tree.

As we read about Rays need for fiction, Powers tells us that novels can only cover a few peoples lives and cannot tell a story that is compelling enough to successfully narrate the world that includes innumerable species, and it is precisely for this reason that we are failing the world. Rather than announcing a cliched return to our earthly natures or the reasons to love trees, The Overstory tells us why it is hard to tell a good story about them.

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How to live longer: How does fasting increase your life expectancy? What we know so far – Express

Posted: February 2, 2020 at 2:45 am

It is well understood that eating a healthy, balanced diet is essential to living a long life, with certain foods offering protection against life-threatening health complications. With the spotlight placed firmly on what foods you should embrace and avoid, less attention has been devoted to the frequency of eating and its impact on longevity.

Pearson continues: It's after this period of time that processes such as autophagy and stem cell generation are triggered.

Autophagy is the body's way of cleaning out damaged cells, in order to regenerate newer, healthier cells, according to Priya Khorana, PhD, in nutrition education from Columbia University.

According to Pearson, profound regenerative changes have been shown with periodic water-only fasting but consuming nothing but water for days on end can be challenging for many.

To circumvent this challenge, professor Valter Longo, who has spearheaded much of the research in the field of fasting and longevity, developed the concept of Fasting Mimicking Diets (FMDs).

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Fast Mimicking Diets mimics fasting by tricking your body into a fasted state, while eating specially designed plant-based mini meals, explains Pearson.

FMDs have been shown to promote multi-system regeneration, enhanced cognitive performance, and health span.

Clinical studies on three, five day FMD cycles, spread over three months, show a spike in circulating stem cells that lead to delayed ageing by promoting regeneration in multiple systems.

Body weight, BMI, total body fat, trunk fat, waist circumference, systolic and diastolic blood pressure, cholesterol, insulinlike growth factor 1 (IGF1) and C-Reactive Protein (a marker of inflammation) were significantly reduced, particularly in participants at risk for diseases.

Curiously, scientists from the University of Wisconsin-Madison, and the Pennington Biomedical Research Center, Baton Rouge, Louisiana, reported that health and longevity improved with increased fasting time, regardless of what the mice ate or how many calories they consumed.

According to the study's lead author, Rafael de Cabo, Ph.D., chief of the Translational Gerontology Branch of the NIA Intramural Research Program, scientists have studied the beneficial effects of caloric restriction for more than a century, but the impact of increased fasting times has recently come under closer scrutiny.

"Increasing daily fasting times, without a reduction of calories and regardless of the type of diet consumed, resulted in overall improvements in health and survival in male mice," said de Cabo.

He added: Perhaps this extended daily fasting period enables repair and maintenance mechanisms that would be absent in a continuous exposure to food."

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Epigenetic Influences and Mechanisms in Asthma – Pulmonology Advisor

Posted: February 2, 2020 at 2:44 am

In an effort to identify genetic variants responsible for asthma, thousands of genetic studies have been performed. Of the potential answers to this dilemma, epigenetics represents a solution, according to a review published in Tuberculosis and Respiratory Diseases.

Familial clustering of asthmatics is indicative of a genetic component of asthma, a condition with up to 60% heritability. Over the last 3 decades, more than 100 loci have been identified as being linked with asthma, and recent genome-wide single nucleotide polymorphism (SNP) association studies have confirmed that SNPs on genes associated with antigen presentation, inflammation, and Th1/Th2 processes are strongly associated with asthma and its subphenotypes.

Worldwide asthma prevalence has increased over the past 30 years, but investigators note that changes in genetic variants of the disease have rarely occurred. They posit that exposure to changing exposomes large sets of environmental exposures including diet, toxins, and hormones have introduced several different phenotypes of asthma.

In 2007, epigenetics was defined via the following criteria: a change in the activity of a gene that does not involve a mutation; initiated by environment signals; and mitotically or meiotically inherited in the absence of the change in nucleotide sequence of genomic DNA. Epigenetic mechanisms include DNA CpG methylation, histone deformation, and non-coding RNA.

DNA methylation takes place in CpG occupying 1% of DNA bases in human somatic cells, resulting in a total number of CpG sites of approximately 28 million; 70% to 80% of the human DNA CpG bases are methylated. Considerable research has identified specific time periods during which individuals are more susceptible to the effects of environmental exposures and other asthma triggers, including during prenatal development, early childhood, and adolescence. Epigenetic modifications are more likely to develop during these times.

Both human skin and lungs have large surface areas exposed to external environments; surface area estimates depend on height, weight, and other factors. Both indoor and outdoor stimuli affect airway epithelium, with 2 methods that have been used to study DNA CpG methylation: candidate gene approaches and epigenome-wide association studies. One candidate CpG methylation approach utilized buccal mucosa of children with asthma on 1505 CpG loci across 807 genes, identifying a small number of DNA methylation signatures. Recent epigenome-wide association studies have been launched with the intention of searching for changes in global CpG methylations.

Many CpG methylation studies have relied on the use of DNA isolated from unfractionated peripheral blood leukocytes because they are widely available and easily accessed. New methods have been recently developed to infer the proportion of immune cell populations in these leukocytes using DNA methylation data. The major immunologic components of asthma pathogenesis and their epigenetic mechanisms specifically affect the expression of the transcription factors involved in the development of Th1, Th2, Th17, and regulatory T cells. Therefore, these DNA methylation profiles have been very useful in identifying the epigenetic change of immune status.

In terms of atmospheric environmental factors, smoking is the most important asthma risk factor. Through DNA damage and other mechanisms, cigarette smoke modulates gene expression; several epigenome-wide association studies have demonstrated that cigarette smoke results in global hypomethylation. In one study, smoke exposure was associated with a 2% increase in mean CpG methylation in the FERM domain containing 4A gene compared with no smoke exposure. Despite the potential for clinical relevance, the authors noted that the current interpretation of cross-sectional epigenetic studies is problematic because it is impossible to determine whether methylation alteration is a cause or consequence.

Because DNA CpG methylation changes are more reversible than DNA mutations, many treatment strategies are currently under investigation, the authors wrote, including dietary manipulation, which has demonstrated that methyl-rich diets have been associated with epigenome hypermethylation. These findings have generated interest, but clinical efficacy is still unclear.

Epigenetic influences and mechanisms have been clarified in allergic diseases and asthma, but there are still many questions to be solved yet, the researchers posit. The most complex situation is when both the gene and the environment are unknown. Additional information on exposomes, they added, is necessary, and data should be analyzed via multi-Omnic approaches.

The researchers concluded that If accurate influence and mechanisms of epigenetics are revealed, prevention and control strategies for asthma and its subtypes will be developed.

Reference

Bae D-J, Jun AE, Chang HS, Park JS, Park C-S. Epigenetic changes in asthma: role of DNA CpG methylation.Tuberc Respir Dis. 2020;83(1):1-13.

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The Mental Game: Inside the mind of an elite athlete – CBS19.tv KYTX

Posted: February 2, 2020 at 2:44 am

With the NFL Championship game this Sunday, all eyes turn to Whitehouse native and Kansas City Chiefs quarterback Patrick Mahomes.

Over the course of the last couple weeks, CBS19 has spoken to everyone from Mahomes father, Pat Mahomes Senior, his trainer at APEC, Bobby Stroupe, and fans across the region. Mahomes environment clearly helped forge him into a future NFL MVP.

Dr. Ushimbra Buford, a psychiatrist at UT Health Science Center at Tyler says there are crucial mental aspects of becoming an elite athlete.

Buford says the phrase nature vs nurture which references conflicting theories on whether the natural instincts with which people are born or the environment in which they are brought up has more of an impact on their development, is now somewhat outdated.

When I first started in medicine, we used to say things like nature and nurture and saw them as two different things. In the past several years with the advent and understanding of epigenetics, we understand now that the nurture aspect influences the nature, the environment influences your genetics, Buford said.

Buford says a persons development in all aspects, including athleticism, is heavily influenced by their environment, down to changes their body makes at a biological level.

From a genetic standpoint, that person may have a certain kind of capacity for the muscle fibers that they will produce that they were born with. But then they get into an environment where the expectation and the understanding and like the daily living is different from what their body is genetically set. Over time, their composition would change to reflect the activities they were involved in, and the potential reality exists that over time their genetics would change to reflect the new physical reality, Buford said.

This means growing up in an environment, such as in Mahomes case, where his father is a professional baseball player and he was immediately around athletics, can be incredibly beneficial. However, Buford says in athletics and in life, it always comes down to a choice to strive for greatness.

Every quarterback is saying we're going to the Super Bowl this year, but how many of them believe that?" Buford said. "How many of them really, truly feel that this is my going to be my reality? You almost have to be delusional like that to achieve greatness in a sense. You have to see and believe something before other people can or will."

This mentality is something everyone can implement in their daily lives. Buford says the sky is the limit when a persons head is in the right place.

We all have the ability to be incredible," Buford said. "You know, we really limit ourselves and I don't know if this is a societal thing, or it's just not receiving the right messages early enough in life. But don't ever think for a minute that anyone cannot become what they want to be. Only person stopping you is you.

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