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Stem Cell Treatments: What to Ask A Closer Look at Stem …

Posted: March 11, 2019 at 10:41 pm

There is certain information you should look into if you are considering a stem cell treatment, including a detailed description of the treatment and the science that supports it, the expected outcome and the risks.

In many cases, particularly in a clinical trial, you should be provided with a patient information sheet and informed consent documents that answer many of the questions below. However, dont hesitate to ask for more information or further explanation. The medical team involved should know a lot about your disease or condition, other treatment options, and the evidence that the treatment they are offering will be safe and that it will work.

The questions and answers are best discussed with a trusted physician familiar with your condition who can help you understand the treatment and your choices. It is a good idea to seek medical advice independent of the provider to help assess whether the treatment and outcome claims offered are reasonable.

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Massages & Treatments | Day Spa on Lake Erie in Ohio | The …

Posted: March 10, 2019 at 3:44 pm

Customized Spa Facial Includes skin analysis, cleansing, exfoliating, treatment mask and a relaxing hand & arm and foot & leg massage to rejuvenate and protect your skin.$89

Mini Facial Includes a deep cleansing with a skin analysis, treatment masque, and moisturizer.$59

Gentlemans Facial Includes a skin analysis, cleansing, treatment mask, and relaxing hand & arm massage to rejuvenate and protect your skin. (Please arrive clean shaven)$79

Seasonal Spa Facial This classic facial includes cleansing, exfoliating, treatment masque, moisturizer, and a relaxing hand & arm massage, featuring seasonally scented Farmhouse Fresh products.$79

Anti-aging FacialThe maximum in anti-aging prevention and hydration. The treatment starts with cleansing and moves to exfoliating treatments, containing plant-derived stem cells, peptides, AHAs and Vitamin C, to provide the maximum in correction, prevention, and nutrition. $99

Signature Lift -Vitamin C and fruit enzymes blended in an organic aloe vera base visibly reduce redness and brighten the skin while hyaluronic acid provides superior hydration. Skin type indications: Redness-prone, dry/dehydrated, smokers skin, tired/ dull, post-microdermabrasion, oily/acne. $89

O2 Lift -This luxurious skin care treatment infuses oxygen, plant-derived stem cells, peptides and a high concentration of enzymatic botanicals into the skin leaving it luminous, refreshed and rejuvenated. $79

Dermaplaining -Amethod of manual exfoliation and removes the outer most layers of dead skin cells and hair leaving the skin immediately smooth, supple and vibrant. $89

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Mutation Generation & Detection HSC Cores: Home

Posted: March 10, 2019 at 3:43 pm

Lonzas 4D Nucleofector Technology is an improved electroporation technology that can help researchers achieve high transfection efficiencies in standard cell lines, primary cells, stem cells, and hard to transfect cell lines. With the 4D Nucleofector high efficiencies can be reached using much lower substrate amounts and with moderate impact on viability. The comprehensive way in which 4D Nucleofector Programs and cell type-specific solutions are developed enables nucleic acid and protein substrate delivery not only to the cytoplasm, but also through the nuclear membrane and into the nucleus. This allows for high efficiencies up to 99% and makes the transfection success independent from cell proliferation.

The MGD Core has acquired a complete Lonza 4D Nucleofector System that any and all researchers can use. This complete System is made up of four unique functional parts:

Core Unit The main control center for the 4D-Nucleofector System that controls the function of all other units. It has a 5.7 touch screen to operate all units and is loaded with intuitive operation software for designing and saving individual experimental setups.

X Unit This base unit allows Nucleofection of cells in suspension in 20ul Nucleocuvette 16-well strips or in single-use 100ul Nucleocuvettes. Each well in a 16-well Nucleocevette strip is electroporated independently allowing for different conditions to be tested and re-use of the strips if wells are not used. This unit is perfect for testing individual conditions on cells and for small-scale experiments.

Y Unit This unit allows Nucleofection of cells while still adherent to 24 well culture plates. This unit is perfect for working with adherent cells, such as neurons derived from stem cells, which are not transfectable in suspension. Transfection of adherent cells using the Y unit may lead to more physiological response in cells.

96-well Shuttle Controlled by the Core unit the 96-well Shuttle is an add-on unit that allows for convenient optimization of conditions or large-scale screens to be preformed. Each individual well is processed independently allowing 96 different experimental conditions to be tested at one time.

The full Lonza 4D Nucleofector System is housed in Room 7470 of the Eccles Institute of Human Genetics, along with a cell culture hood for researchers to work with their cells in and a 37C incubator to store their cell.

To reserve time to use the Lonza 4D Nucleofector System please use the following link to login to the HSC Core Research Facilities resource page. Select the Mutation Generation page option and then select the 4D Nucleofector page option. Here you can reserve time to use the System. Researchers will be charged a $5.00 fee for every 30 minutes block reserved. Individual experiments do not require more than one 30-minute block.

University of Utah Core Labs

Lonza maintains two databases with protocols (including Nucleofector Solution types and program numbers) of optimized protocols for a wide range of cell lines. Basically Lonza has already done the optimization experiment and determined the best conditions to achieve the highest transfection efficiency with the least amount of cell death. These databases are a good starting place to determine the most optimal protocol for working with your specific cell line.

Lonzas public optimized cell line protocol database can be found at the following link: http://bio.lonza.com/6.html

Lonza also maintains a database of user-optimized protocols that is not publicly available. Please contact either Dr. Gregory Alberts or Haylee Erickson at the following information for access. Dr. Alberts is an expert on the use of the 4D Nucleofector System and a great resource for the best transfection protocols to use with your specific cell line. The following is a link to a seminar given by Dr. Alberts on using the 4D Nucleofection system:Dr. Alberts 4D Nucleofection System

Gregory Alberts, Ph.D. gregory.alberts@lonza.com

Global Subject Matter Expert

Lonza Pharma Bioscience Solutions

Haylee Erickson haylee.erickson@lonza.com

Sales Specialist, Rocky Mnt/Pacific NW

Lonza Pharma Bioscience Solutions

Original CRISPR-Cas9 experiments were performed using DNA vectors, viral vectors or RNA transfection to produce the components of the system: Cas9 protein and single guide RNAs (sgRNAs). New advances have demonstrated that these component can be produced and combined in vitro to form a ribonucleoprotein complex or RNP that is functional in vitro and in vivo without the need for transcription or translation. This CRISPR RNP complex a can be delivered directly to cells and results in immediate, efficient, and specific target cleavage by the CRISPR RNP.

Several labs have shown that combining the CRISPR RNP approach with the extremely high transfection efficiency of the Lonza 4D Nucleofection System can result in mutation frequencies reaching 90% of targeted gene copies in several different cell types. CRISPR RNP delivery is applicable to a wide range of cell types, including established cell lines, primary cells, adherent cells such as primary neurons, iPCS, and stem cells. With these cell types using the CRISPR RNP approach can dramatically shortened the time it takes to create targeted variants of your gene of.

Please contact the MGD Core if you have any questions concerning this approach or would like to discuss the possibility of using CRISPR RNP in your research. Also, the following link is a generalized protocol detailing how to combine the CRISPR RNP approach with the Lonza 4D Nucleofection System.

General Nucleofection Protocol

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Top Stem Cell Conferences | Stem Cell Research 2019 …

Posted: March 10, 2019 at 3:42 pm

Session and TracksTrack 1: Stem Cells Biology

Stem cells are defined as precursor cells that have the capability to self-renew and to come up with multiple mature cell types. Stem cells are an ongoing source of the differentiated cells that make up the tissues and organs of plants and animals. After collecting and culturing tissues is it possible to classify cells as per their operational concept. There are 2 major types of stem cells: Embryonic Stem Cells and Adult Stem Cells that is also known as tissue stem cells. This difficulty in characteristic stem cells in situ, without any manipulation, limits the understanding of their true nature. There is great interest in stem cells as a result of they have potential in the development of therapies for replacing defective or damaged cells ensuing from a variety of disorders and injuries, like Parkinson disease, heart disease, and diabetes.

Related Conferences:

25th Global Meet on Cancer Research & Oncology, May 20-21, 2019, Rome, Italy; 2nd World Congress on Advanced Cancer Science & Therapy, January 28-29, 2019, Dubai, UAE; 3rd Advances in Cell & Stem Cell Research Congress, September 25-26, 2019, Rome, Italy; 3rd International Conference on Nanostructures, Nanomaterials and Nanoengineering, October 21-22, 2019, Las Vegas, USA; 3rd World Congress on Advanced Biomaterials and Tissue Engineering, August 26-27, 2019, Madrid, Spain;

Hematopoietic Stem Cells are the immature cell that is developed into all types of blood cells, including red blood cells, white blood cells, and platelets which are found in the peripheral blood and the bone marrow. These stem cells are also called blood stem cell. Studies have described two populations of Hematopoietic Stem Cells that are Long Term and Short Term. Long-Term Hematopoietic stem cells which are capable of self-renewal, while Short Term Hematopoietic stem cells do not have this capacity.

Related Conferences:

4th World Biotechnology Congress, May 20-21, 2019, London, UK; 6th World Congress on Microbial Biotechnology, June 17-18, 2019, Paris, France; Annual Congress on Advanced Tissue Science and Regenerative Medicine, April 15-16, 2019, Amsterdam, Netherlands; World Congress on Cell & Gene Therapy, September 25-26, 2019, Rome, Italy; World Congress on Novel Trends and Advances in Biotechnology, September 25-26, 2019, Rome, Italy;

Embryonic Stem Cells are developed when embryos formed during the blastocyst phase of embryological development. They can grow in all derivatives of the 3 primary germ layers i.e. ectoderm, endoderm and mesoderm. These include each of the more than 220 cell varieties within the adult body. Pluripotency distinguishes embryonic stem cells from adult stem cells found in adults; whereas embryonic stem cells can generate all cell types within the body, adult stem cells are multipotent and can produce only a restricted number of cell types. Embryonic stem cells are capable of propagating themselves indefinitely. This allows embryonic stem cells to be employed as useful tools for both research and regenerative medicine, because they can produce limitless numbers of themselves for continued research or clinical use.

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Induced Pluripotent Stem Cells (iPSCs) are the adult stem cells derived from skin or blood cells which are reprogrammed to an embryonic stem cell maintaining the essential properties of introducing important genes and also to enables the development of an unlimited source of any type of human cell needed for the therapeutic purpose. Researchers have rapidly developed the techniques for generating iPSCs and by creating a new and powerful way to "de-differentiate" cells whose developmental fates.

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Though the concept of stem cell niche was prevailing in vertebrates, the first characterization of stem cell niche in vivo was figured out in drosophila germinal development. A stem-cell niche is an area of a tissue that provides a specific microenvironment, in which stem cells are present in an undifferentiated and self-renewable state. Cells of the stem-cell niche interact with the stem cells to take care of them or promote their differentiation. Characterization of these stem cell niches depends on the ability to identify stem cells in vivo in their normal setting. Through comparison of different stem cell systems, some themes emerge that indicate possible general characteristics of the relationship between stem cells and their supporting niche.

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Stem cell banking is the extraction, processing and storage of stem cells which can used for treatment when required. Stem cells have the amazing power to transform into any tissue or organ in the body. It is due to this unique characteristic that they have the potential to treat over 80 life threatening diseases, and provide numerous benefits to the baby, its siblings and the family. There are variety of sources from where stem cells can be banked, with the most common amongst them being the umbilical cord. Cord blood banking is that the extraction of stem cells from the umbilical cord. This is done during childbirth and is a fast, hassle free and painless procedure. While, the umbilical cord and cord blood are the foremost common sources of stem cells - the Placenta, amniotic sac and amniotic fluid are by far the richest sources, in terms of both - quantity and quality. Some other rich sources of stem cells are Placenta, Umbilical Cord, Amniotic Fluid, Dental Stem Cells, Menstrual Fluid, Adipose Tissue and Bone Marrow.

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A procedure in which a patient receives healthy blood-forming cells (stem cells) to replace their own, when stem cells or bone marrow are damaged or destroyed by disease, as well as some types of cancer, or by high doses of chemotherapy or radiation therapy used to treat cancer. The healthy stem cells may come from the blood or from a donors bone marrow or from the umbilical cord blood of a newborn baby. A stem cell transplant may be autologous (use of stem cells from your own bone marrow or blood), allogeneic use of stem cells from someone else, the donor could also be a relative or somebody who isn't associated with you) or syngeneic (use of stem cells from an identical twin). The stem cells within the bone marrow transform into red blood cells, white blood cells and platelets. when these blood cells mature, they go into the peripheral blood (the blood that flows through the body). If the bone marrow is damaged or destroyed, it cant create normal blood cells. in a stem cell transplant, healthy stem cells are placed in your body to assist your bone marrow start to work properly. The new stem cells make healthy blood cells.

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Stem Cell Therapy is the treatment for various disorders which non-seriously life-threatening by using stem cells. These stem cells can be obtained from a lot of different sources and used to potentially treat more than 80 disorders which include neuromuscular, organ, chronic and degenerative disorders. Chronic disorders arise from degeneration or wear and tear of cartilage, muscle, bone, fat or the opposite organ, tissue or cell. This may occur owing to a spread of reasons, but it's usually the tactic spoken as aging, or 'getting old' that is the largest cause. Stem cell therapy is currently being researched for the treatment of various diseases. While research and clinical trials are in process with varying degrees of success, stem cell therapy holds the potential to offer a successful cure for these conditions.

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Cancer is defined as the abnormal growth of cells that possesses the ability to spread to other cells and tissues. Cancer is one of the major illness which it seemed to be more prevalent all over the world. Even though the death rate and peoples suffering from these diseases are in greater number in recent years. There are over 200 variety of types of cancer across the globe. The death rate increasing year-by-year due to this disease even in developed countries. Cancer Stem Cells (CSCs) are a small population of cells inside tumors with capabilities of self-renewal, differentiation, and tumorigenicity once transplanted into an animal host. The CSC hypothesis thus doesn't imply that cancer is always caused by stem cells or that the potential application of stem cells to treat conditions like cardiovascular disease or diabetes which is able to result in tumor formation. Rather, tumor-initiating cells possess stem-like characteristics to a degree sufficient to warrant the comparison with stem cells along with the observed experimental and clinical behaviors of metastatic cancer cells are extremely resembling the classical properties of stem cells

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As an organism grows and develops, carefully orchestrated chemical reactions activate and deactivate components of the genome at strategic times and in specific locations. Epigenetics is that the study of these chemical reactions and the factors that influence them. It is strongly believed that there are some signals at the epigenetic level that regulate the fate of the stem cells. Though all of the cells in our body contain the same genetic makeup. These genes are not necessarily active at all times, rather they are expressed at times when needed in a highly controlled fashion.

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Tissue Engineering is a scientific field centered on the advance of Tissue and Organ Substitutes by controlling their environment, biomechanical and biophysical parameters which include the utilization of a different or same scaffold for the arrangement of new tissue. These frameworks empower the In-vitro investigation of human physiology and physiopathology, while giving a rendezvous of biomedical instruments with potential materialness in toxicology, medicinal gadgets, tissue substitution, repair and Regenerative Medicine. Regeneration is that the progression of renewal, regeneration, and growth that makes it cells, organ regeneration to natural changes or events that cause damage or disturbance. This study is carried out as craniofacial tissue engineering, in-situ tissue regeneration, adipose-derived stem cells for tissue science which is also a breakthrough in cell culture technology. The study isn't stopped with the regeneration of tissue wherever it is further carried out in relation to cell signaling, morphogenetic proteins. Most of the neurological disorders occurred accidentally having a scope of recovery by replacement or repair of intervertebral discs repair, spinal fusion and plenty of more advancement.

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Regeneration Medicine is that the Survival of any living body essentially relying upon its capability to repair and recover injured or harmed tissues or potential organs amid its lifespan following injury, illness, or maturing. This will shape the system for recognizing novel clinical medicines which will enhance the mending and regenerative limit of individuals. The Regeneration process involves Cell Proliferation where most of the medical disorders occurred accidentally includes a scope of recovery by replacement or repair of intervertebral discs repair, spinal fusion and plenty of more advancements.

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Cell reprogramming is the process of reverting mature, specialized cells into induced pluripotent stem cells. Reprogramming also refers to the erasure and re-establishment of epigenetic marks during mammalian germ cell development. The discovery of Induced pluripotent stem cells emphasizes on reprograming of any adult differentiated cells into stem cells by genetic modification under precisely controlled laboratory conditions. Reprograming of cells is supposed to presage revolution in both, medical and biological research and allows modeling and analysis of human diseases and cell cytotoxicity by drugs. The technique is still in its growing phase and requires a great deal of extensive research and approval from authorities for further trials.

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Stem cell nanotechnology has emerged as a brand-new exciting field. Experimental and theoretical studies of interaction between nanostructures or nanomaterials and stem cells have made great advances. The importance of nanomaterials, nanostructures, and nanotechnology to the basic developments in stem cells-based therapies for injuries and degenerative diseases has been recognized. Apart from tracking the localization of stem cells, nanotechnology has improved targetability, half-life, and stability of stem cells by providing a suitable microenvironment. In particular nanomaterials have played a significant role in the isolation and proliferation or differentiation of stem cells and intracellular delivery of small and macromolecules within stem cells. In this field over the past few years, explore the application prospects, and discuss the issues, approaches and challenges, with the aim of rising application of nanotechnology in the stem cells research and development.

Related Conferences:

Stem Cell Technologies will become a global biotechnology that manufactures, develops and sells product by providing the services to support academic and industrial scientists. Stem cells analysis and development team typically collaborates with educational institutes and industrial partners to manufacture, develop and distribute a specific product for a given analysis. A stem cell has helped several scientific communities and industries to develop technologies to achieving the world biotechnology market. The corporate makes a specialist in developing cell culture media, cell separation product, instruments and completely different reagents to be utilized in the cell, immunology, cancer, Regenerative medicine and cellular treatment analysis.

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There are many research advancements and applications and of Stem Cells. Stem cell research that can be applied to develop new therapies includes cell replacement therapy, development of drugs, using iPSC technology to generate stem cells from the patients skin or blood, using trans differentiation technology to convert a specialized cell type to a progenitor cell and many more. It also carries the immense potential for treating a number of human diseases such as to repair or regenerate blood vessels, treatment of eyesight, Diabetes, Neurodegenerative Disorders and Wound Healing etc.

Related Conferences:

Carefully planned and ethically approved clinical trials resulting from a robust preclinical pathway are necessary to advance the field. This will require a programmatic approach that involves partnerships of clinicians, academics, industry, and regulatory authorities with a focus on understanding basic biology that informs a tight linkage between preclinical and clinical studies. Rather than suggesting that clinical trials are premature, such trials should be encouraged as part of multidisciplinary programs in regenerative medicine.

Related Conferences:

The field of bioethics has addressed a broad swathe of human inquiry, ranging from debates over the boundaries of life, surrogacy, and the allocation of scarce health care resources to the right to refuse medical care for religious or cultural reasons. StemGen is a research database of international, regional and national normative instruments concerning the socio-ethical and legal aspects of stem cell research and related therapies. The regulation of stem cell research is an issue that has drawn much comment, criticism and even judicial arbitration in recent years along with the marketing status of Stem Cells, Cell therapy, Regenerative Medicine, Tissue Engineering and many more worldwide.

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Genetic engineering | Memory Alpha | FANDOM powered by Wikia

Posted: March 10, 2019 at 3:42 pm

A portrait of Khan Noonien Singh, a man who was a product of genetic engineering

Genetic engineering, genetic programming or genetic manipulation was a process in which the DNA of an organism was selectively altered through artificial means. Genetic engineering was often used to produce "custom" organisms, such as for agricultural or medical purposes, as well as to produce biogenic weapons. The most common application of genetic engineering on intelligent beings in the Federation was corrective DNA resequencing for genetic disorders. A far more dubious application of genetic engineering was the genetic enhancement of individuals to produce improved senses, strength, intelligence, etc.

During Earth's 20th century, the efforts of ambitious scientists' to produce "superhumans" eventually resulted in the Eugenics Wars. Genetically engineered individuals such as Khan Noonien Singh attempted to seize power. (TOS: "Space Seed")

This would lead to the banning of genetic engineering on Earth by the mid-22nd century, even research which could be used to cure critical illnesses. This ban was implemented because of the general fear of creating more tyrants such as Khan. It was also felt that parents would feel compelled to have their children genetically engineered, especially if "enhanced" individuals were allowed to compete in normal society.

Some, including geneticist Arik Soong, argued that it was simply convenient for Humanity to denounce the attempts at genetic "improvement" of Humanity, that it was inherently evil because of the Eugenics Wars. He argued that the source of the problem, in fact, wasn't the technology, but Humanity's own inability to use it wisely. Imprisoned for, among other crimes, stealing the embryos of a number of Augment children, Soong wrote long treatises on the subject of genetic augmentations and improvements. His works were routinely taken and placed into storage (although his jailers often told him that his work was vaporized). Though Soong himself gave up genetics to begin research in cybernetics, Captain Jonathan Archer expressed his hope to Soong that research into genetic engineering could cure life-threatening diseases would someday be resumed. (ENT: "Borderland", "The Augments")

Symbols used to indicate presence of genetically engineered lifeform

Others, however, chose to establish isolated colonies, as became the case with the Genome colony on Moab IV, which was established in 2168. It became a notable and successful example of Human genetic engineering in which every individual was genetically tailored from birth to perform a specific role in society. However, after a five-day visit by the USS Enterprise-D when the ship came to the colony in an effort to save it from an approaching neutron star which, eventually, the craft was able to effectively redirect twenty-three colonists left the colony aboard the craft, possibly causing significant damage to the structure of their society. The reason for the societal split was that those who left the colony had realized their organized, pre-planned world had certain limitations, lacking opportunities to grow that were offered by the Enterprise. (TNG: "The Masterpiece Society")

By the 24th century, the United Federation of Planets allowed limited use of genetic engineering to correct existing genetically related medical conditions. Persons known to be genetically enhanced, however, were not allowed to serve in Starfleet, and were especially banned from practicing medicine. (TNG: "Genesis", DS9: "Doctor Bashir, I Presume")

Nevertheless, some parents attempted to secretly have their children genetically modified. (DS9: "Doctor Bashir, I Presume") Unfortunately, most of these operations were performed by unqualified physicians, resulting in severe psychological problems in the children due to their enhancements being only partially successful, such as a patient's senses being enhanced while their ability to process the resulting data remained at a Human norm. (DS9: "Statistical Probabilities")

In some cases, genetic engineering can be permitted to be performed in utero when dealing with a developing fetus to correct any potential genetic defects that could handicap the child as they grew up. Chakotay's family history included a defective gene that made those who possessed it prone to hallucinations, the gene afflicting his grandfather in Chakotay's youth, although the gene was suppressed in Chakotay himself. (VOY: "The Fight") In 2377, The Doctor performed prenatal genetic modification on Miral Paris to correct a spinal deviation, a congenital defect that tends to run in Klingon families; Miral's mother and grandmother had undergone surgery to correct the defect at a young age, but the modification meant Miral would not need surgery herself. Unfortunately, learning of this capability, B'Elanna briefly became obsessed with having her child modified to remove all Klingon DNA traits to try and 'protect' her daughter from the discrimination she had experienced as a child, even going so far as to reprogram The Doctor so that he would believe these changes were necessary to prevent later illness, but she was talked out of it by her husband (VOY: "Lineage").

The Founders of the Dominion performed extensive genetic modifications on their two servant races, the Jem'Hadar and the Vorta, in order for them to better serve their roles and to ingrain a fanatical devotion to the Founders. (DS9: "The Abandoned", "Ties of Blood and Water") As a result of these modifications, neither species reproduced in the traditional biological sense. (DS9: "To the Death")

According to Vorta legend, they were originally ape-like creatures who were gifted sentience by the Founders after they helped a changeling escape pursuit. (DS9: "Treachery, Faith and the Great River")

It is unknown whether the Jem'Hadar had any such ancestral species.

The Dominion also genetically engineered biological weapons, such as the blight they unleashed against the people of the Teplan system. (DS9: "The Quickening")

During the 22nd century, the Suliban were no more evolved than Humans. However, a number of Suliban, from a faction known as the Suliban Cabal, became recipients of some very sophisticated genetic engineering thanks to a mysterious humanoid from the 28th century. These enhancements included subcutaneous pigment sacs, a bio-mimetic garment, modified alveoli, more bronchial lobes and eyes with compound retinas which allowed them to see things starship sensors likely could not detect. The Suliban considered these "enhancements" as "progress". (ENT: "Broken Bow")

When they were captured by a pre-warp civilization in 2152, Jonathan Archer and Malcolm Reed claimed to be prototypes of a new breed of supersoldiers to conceal the existence of alien life from the civilization. (ENT: "The Communicator")

Genetic engineering had been employed on Denobula since the twentieth century, to generally positive effect. (ENT: "Borderland")

Genetic programming was Surmak Ren's major field of study at the University of Bajor. (DS9: "Babel")

The Angosians used psychological and biochemical modifications and mental programming to make the perfect soldier such as Roga Danar. (TNG: "The Hunted")

The Tosk were engineered by the Hunters to be prey for their traditional hunts. (DS9: "Captive Pursuit")

The Son'a used genetic manipulation as part of a range of strategies to retard aging. (Star Trek: Insurrection)

The Brunali were proficient at genetic engineering, which they used to create modified crops capable of surviving on their Borg-devastated homeworld. However, they also genetically engineered some of their children to produce a pathogen deadly to Borg. These children were then allowed to be assimilated, so that they could spread the infection to their Borg vessels. Icheb was one such child, the pathogen causing the cube that he was on to break down, killing all of the active drones and causing the young drones in their maturation chambers to activate before they were fully processed into the Collective. (VOY: "Child's Play")

The Taresians used genetic engineering in tandem with a form of biological weaponry to manipulate the DNA of other species. This occurred to Ensign Harry Kim in 2373, who was infected with a virus that altered his DNA to make him a potential Taresian mate. (VOY: "Favorite Son")

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CRISPR: A game-changing genetic engineering technique …

Posted: March 10, 2019 at 3:42 pm

Have you heard? A revolution has seized the scientific community. Within only a few years, research labs worldwide have adopted a new technology that facilitates making specific changes in the DNA of humans, other animals, and plants. Compared to previous techniques for modifying DNA, this new approach is much faster and easier. This technology is referred to as CRISPR, and it has changed not only the way basic research is conducted, but also the way we can now think about treating diseases [1,2].

CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeat. This name refers to the unique organization of short, partially palindromic repeated DNA sequences found in the genomes of bacteria and other microorganisms. While seemingly innocuous, CRISPR sequences are a crucial component of the immune systems [3] of these simple life forms. The immune system is responsible for protecting an organisms health and well-being. Just like us, bacterial cells can be invaded by viruses, which are small, infectious agents. If a viral infection threatens a bacterial cell, the CRISPR immune system can thwart the attack by destroying the genome of the invading virus [4]. The genome of the virus includes genetic material that is necessary for the virus to continue replicating. Thus, by destroying the viral genome, the CRISPR immune system protects bacteria from ongoing viral infection.

Figure 1 ~ The steps of CRISPR-mediated immunity. CRISPRs are regions in the bacterial genome that help defend against invading viruses. These regions are composed of short DNA repeats (black diamonds) and spacers (colored boxes). When a previously unseen virus infects a bacterium, a new spacer derived from the virus is incorporated amongst existing spacers. The CRISPR sequence is transcribed and processed to generate short CRISPR RNA molecules. The CRISPR RNA associates with and guides bacterial molecular machinery to a matching target sequence in the invading virus. The molecular machinery cuts up and destroys the invading viral genome. Figure adapted from Molecular Cell 54, April 24, 2014 [5].

Interspersed between the short DNA repeats of bacterial CRISPRs are similarly short variable sequences called spacers (FIGURE 1). These spacers are derived from DNA of viruses that have previously attacked the host bacterium [3]. Hence, spacers serve as a genetic memory of previous infections. If another infection by the same virus should occur, the CRISPR defense system will cut up any viral DNA sequence matching the spacer sequence and thus protect the bacterium from viral attack. If a previously unseen virus attacks, a new spacer is made and added to the chain of spacers and repeats.

The CRISPR immune system works to protect bacteria from repeated viral attack via three basic steps [5]:

Step 1) Adaptation DNA from an invading virus is processed into short segments that are inserted into the CRISPR sequence as new spacers.

Step 2) Production of CRISPR RNA CRISPR repeats and spacers in the bacterial DNA undergo transcription, the process of copying DNA into RNA (ribonucleic acid). Unlike the double-chain helix structure of DNA, the resulting RNA is a single-chain molecule. This RNA chain is cut into short pieces called CRISPR RNAs.

Step 3) Targeting CRISPR RNAs guide bacterial molecular machinery to destroy the viral material. Because CRISPR RNA sequences are copied from the viral DNA sequences acquired during adaptation, they are exact matches to the viral genome and thus serve as excellent guides.

The specificity of CRISPR-based immunity in recognizing and destroying invading viruses is not just useful for bacteria. Creative applications of this primitive yet elegant defense system have emerged in disciplines as diverse as industry, basic research, and medicine.

In Industry

The inherent functions of the CRISPR system are advantageous for industrial processes that utilize bacterial cultures. CRISPR-based immunity can be employed to make these cultures more resistant to viral attack, which would otherwise impede productivity. In fact, the original discovery of CRISPR immunity came from researchers at Danisco, a company in the food production industry [2,3]. Danisco scientists were studying a bacterium called Streptococcus thermophilus, which is used to make yogurts and cheeses. Certain viruses can infect this bacterium and damage the quality or quantity of the food. It was discovered that CRISPR sequences equipped S. thermophilus with immunity against such viral attack. Expanding beyond S. thermophilus to other useful bacteria, manufacturers can apply the same principles to improve culture sustainability and lifespan.

In the Lab

Beyond applications encompassing bacterial immune defenses, scientists have learned how to harness CRISPR technology in the lab [6] to make precise changes in the genes of organisms as diverse as fruit flies, fish, mice, plants and even human cells. Genes are defined by their specific sequences, which provide instructions on how to build and maintain an organisms cells. A change in the sequence of even one gene can significantly affect the biology of the cell and in turn may affect the health of an organism. CRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism.

Rather than relying on bacteria to generate CRISPR RNAs, scientists first design and synthesize short RNA molecules that match a specific DNA sequencefor example, in a human cell. Then, like in the targeting step of the bacterial system, this guide RNA shuttles molecular machinery to the intended DNA target. Once localized to the DNA region of interest, the molecular machinery can silence a gene or even change the sequence of a gene (Figure 2)! This type of gene editing can be likened to editing a sentence with a word processor to delete words or correct spelling mistakes. One important application of such technology is to facilitate making animal models with precise genetic changes to study the progress and treatment of human diseases.

Figure 2 ~ Gene silencing and editing with CRISPR. Guide RNA designed to match the DNA region of interest directs molecular machinery to cut both strands of the targeted DNA. During gene silencing, the cell attempts to repair the broken DNA, but often does so with errors that disrupt the geneeffectively silencing it. For gene editing, a repair template with a specified change in sequence is added to the cell and incorporated into the DNA during the repair process. The targeted DNA is now altered to carry this new sequence.

In Medicine

With early successes in the lab, many are looking toward medical applications of CRISPR technology. One application is for the treatment of genetic diseases. The first evidence that CRISPR can be used to correct a mutant gene and reverse disease symptoms in a living animal was published earlier this year [7]. By replacing the mutant form of a gene with its correct sequence in adult mice, researchers demonstrated a cure for a rare liver disorder that could be achieved with a single treatment. In addition to treating heritable diseases, CRISPR can be used in the realm of infectious diseases, possibly providing a way to make more specific antibiotics that target only disease-causing bacterial strains while sparing beneficial bacteria [8]. A recent SITN Waves article discusses how this technique was also used to make white blood cells resistant to HIV infection [9].

Of course, any new technology takes some time to understand and perfect. It will be important to verify that a particular guide RNA is specific for its target gene, so that the CRISPR system does not mistakenly attack other genes. It will also be important to find a way to deliver CRISPR therapies into the body before they can become widely used in medicine. Although a lot remains to be discovered, there is no doubt that CRISPR has become a valuable tool in research. In fact, there is enough excitement in the field to warrant the launch of several Biotech start-ups that hope to use CRISPR-inspired technology to treat human diseases [8].

Ekaterina Pak is a Ph.D. student in the Biological and Biomedical Sciences program at Harvard Medical School.

1. Palca, J. A CRISPR way to fix faulty genes. (26 June 2014) NPR < http://www.npr.org/blogs/health/2014/06/26/325213397/a-crispr-way-to-fix-faulty-genes> [29 June 2014]

2. Pennisi, E. The CRISPR Craze. (2013) Science, 341 (6148): 833-836.

3. Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D.A., and Horvath, P. (2007). CRISPR provides acquired resistance against viruses in prokaryotes. Science 315, 17091712.

4. Brouns, S.J., Jore, M.M., Lundgren, M., Westra, E.R., Slijkhuis, R.J., Snijders, A.P., Dickman, M.J., Makarova, K.S., Koonin, E.V., and van der Oost, J. (2008). Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321, 960964.

5. Barrangou, R. and Marraffini, L. CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity (2014). Molecular Cell 54, 234-244.

6. Jinkek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. (2012) 337(6096):816-21.

7. CRISPR reverses disease symptoms in living animals for first time. (31 March 2014). Genetic Engineering and Biotechnology News. <http://www.genengnews.com/gen-news-highlights/crispr-reverses-disease-symptoms-in-living-animals-for-first-time/81249682/> [27 July 2014]

8. Pollack, A. A powerful new way to edit DNA. (3 March 2014). NYTimes < http://www.nytimes.com/2014/03/04/health/a-powerful-new-way-to-edit-dna.html?_r=0> [16 July 2014]

9. Gene editing technique allows for HIV resistance? <http://sitn.hms.harvard.edu/flash/waves/2014/gene-editing-technique-allows-for-hiv-resistance/> [13 June 2014]

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How A "Full Body Stem Cell Makeover" Works – Ben …

Posted: March 9, 2019 at 6:53 am

Note from Ben regarding the Full Body Stem Cell Makeover: I mentioned 30K as total cost on the podcast.That actually is the cost for all the stem cell and exosome injectionsinto the spine and joints.The *cosmetic* and *sexual* add-ons tack 12-15K onto the total cost of this procedure. Just a heads up!

What you are about to discover in this podcast is one of the most advanced, fringe, cutting-edge biohacks and forays into self-experimentation I've ever done

a full body stem-cell makeover at Docere Clinics in Park City, Utahincluding a little-known type of compound called an exosome.

Dr. Harry Adelson, my firstguest on today's podcast,was one of the early adopters of stem cell therapy for the treatment of pain. He began his training in regenerative injection therapy (prolotherapy) in 1998 while in his final year at The National College of Naturopathic Medicine, in Portland, Oregon after having been cured of a rock-climbing injury with prolotherapy. During his residency program in Integrative Medicine at the Yale/Griffin Hospital in Derby, Connecticut, he volunteered after hours in a large homeless shelter in Bridgeport, Connecticut, providing regenerative injection therapies to the medically underserved while gaining valuable experience. He opened Docere Clinics in Park City in 2002 and from day one, his practice has been 100% regenerative injection therapies for the treatment of musculoskeletal pain conditions. In 2006 he incorporated platelet-rich plasma and ultrasound-guided injection into his armamentarium, in 2010, bone marrow aspirate concentrate and adipose-derived stem cells, and in 2013, fluoroscopic-guided injection (motion X-ray).

Since February of 2010, Dr. Adelson has performed over 5,000 bone marrow and adipose-derived adult stem cell procedures and has injected stem cells into over 500 intervertebral discs, placing him among those most experienced in the world with the use of autologous stem cells for the treatment of musculoskeletal pain conditions.

Dr. Amy Killen is also a guest on today's show. She joined Docere Medical after moving to Utah from Portland, Oregon, where she was the Medical Director of an anti-aging and regenerative medicine specialty practice. Dr. Killens medical training began in Dallas, where she attended medical school at UT Southwestern and received her M.D. degree, graduating in the top 10% of her class. She completed a residency in Emergency Medicine at the University of Arizona and served as Chief Resident during her final year. After working as a board-certified emergency physician for more than 7 years, Dr. Killen began studying and training in anti-aging, regenerative, and aesthetic medicine in hopes of approaching patients wellness and beauty from a different perspective and with a new toolbox of innovative and effective treatment options.

Advanced training has included completion of the fellowship in Anti-Aging and Regenerative Medicine through the American Association of Anti-Aging Medicine and certification training in Age Management Medicine. Dr. Killen is certified by the National Institute of Medical Aesthetics in both basic and advanced cosmetic injections and trained with Dr. Lisbeth Roy to learn the O-Shot and P-Shot PRP procedures. Further PRP injection training was provided by PRP Life Lift and she completed the Hair Coach certification program with Dr. Alan Bauman in Florida, which taught a comprehensive approach to non-surgical hair loss. Dr. Killen learned how to extract and process stem cells as part of the U.S. Stem Cell Training Course taught by leading stem cell scientist Dr. Kristin Comella. Dr. Amy Killen has also spent many hours in the operating suite learning directly from Harry Adelson N.D.at Docere Clinics.

Dr. Killen has spoken nationally about PRP and stem cell therapies and teaches a physician training course for ApexBiologix outlining current best practices for using regenerative therapies in aesthetics and sexual optimization.

During my discussion with Harry and Amy, you'll discover:

-How Harry got started with prolotherapy and PRP for pain management in farmers and construction workers14:45

-The Stem Cell Theory of Aging, which states that biologic aging is a result of loss function and population of stem cells in musculoskeletal tissues24:00

-How Harry is harvesting stem cells from bone marrow, supplementing them with exosomes (the currency of stem cells, the inter-cellular communication blocks), and then injecting them into all major moving parts as well as skin of face, scalp, and penis28:30

-Why umbilical and amniotic stem cells could potentially be dangerous due to exposing the body to foreign DNA32:30

-What MSC's are, why are they different and better than other stem cell therapies, and how they differentiate into musculoskeletal tissues and have been called medical signaling cells because they trigger the healing of damaged tissues42:00

-Why somebody would undergo a full body stem cell treatment, and whether is it going to rejuvenate all of the cells in my body49:30

-What to expect after a full body stem cell makeover57:00

Interview with Dr. Amy Killen:

-WhatPRP therapy is, and how it differs from stem cells1:07:00

-What happens when you inject stem cells into a mans unit, and why Amy injected Ben1:11:30

-Why Amy injects stem cells and PRP into vaginas, and how the O-Shot improves the power and duration of female orgasms1:15:20

-The secrets of the vampire breast lift and other cosmetic procedures she has done1:18:15

-What causes hair loss, and how to fix it with stem cells1:22:15

-What V-cells are and how they work1:27:00

-Post-treatment care and biohacks to enhance recovery and efficacy of the treatment1:29:00

-And much more!

Click here for the full written transcript of this podcast episode.

Resources from this episode:

Docere Clinics in Park City, Utah

-Video:What Are Exosomes? Ben Greenfield Interviews Dr. Harry Adelson On Full Body Stem Cell Makeover

-Video:Ben Greenfield Interviews Dr. Amy Killen About Stem Cells, PRP, Exosomes & P-Shot

-Video:Ben Greenfield Full Body Stem Cell Makeover With Exosomes: Part 1

-Video:Ben Greenfield Full Body Stem Cell Makeover With Exosomes: Part 2

-All the recovery biohacks I implemented to recover from the stem cell procedures and enhance stem cell production:

Flexpulse PEMFPulsecenters PEMFBiomat InfraredTrusii Hydrogen Rich WaterJoovv Infrared LightNanoVi DNA RepairExogenous HVMN KetonesOmega-3 DHA Superessential Fish OilVielight PhotobiomodulationKion FlexClearlight Sauna

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Do you have questions, thoughts or feedback for Dr. Harry, Amy or me? Leave your comments below and one of us will reply!

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Marshfield Clinic Research Institute – Welcome to The …

Posted: March 9, 2019 at 6:51 am

The Center for Precision Medicine Research (CPMR), formerly the Center for Human Genetics,wasestablished in 2004 and focuses its research on discovering the structure of the human genome and the hunt for genes that influence human health disorders. The discoveryof short tandem repeat polymorphisms in 1989revolutionized the study of human genetics, and theMarshfield mapsare among the most reliable and widely used maps of the human genome in the world.Thecentercomprises oftwo of Marshfield Clinics internationally known research assets: the Center for Medical Genetics, founded in 1994, and the Personalized Medicine Research Center, started in 2001.Scientists and CPMR staffnow focus on multiple areas of study including Personalized Medicine Research Project (PMRP) study, Precision Medicine Initiative (PMI) study and All of Us (AoU) Research Program.

In 2002,CPMRlaunchedthe largest population-based genetic research project in the United States, involving more than 20,000 central Wisconsin residents, the Personalized Medicine Research Projector PMRP. By understanding which genes and environmental factors are involved in disease and cancer, doctors might be able to better target the biological pathways involved. This information may also enable doctors to predict disease risk and prescribe preventative measures.

The Marshfield Clinic isone of four partners in the Wisconsin Genomics Initiative, a historic collaboration, whose vision is to be able to predict, for individual patients in a clinical setting, the risks of disease susceptibility and treatment response using the combined power of cutting edge genetic, phenotypic, and environmental analysis. Each of the four partners is a leader in one or more areas needed for a successful effort.

A team of investigators and staff in the Center for Precision Medicine Research, led by Scott Hebbring, PhD, are conducting a pilot study that applies genetic testing results to improve and personalize care for 2,000 Marshfield Clinic patients. It is estimated that up to 3% of patients will carry a genetic variant that is clinically actionable. Clinically actionable variants are those that increase disease risk for conditions where early detection may improve outcomes such as cancer and heart disease. In addition to disease risk variants, it is expected that 95% of patients will carry one or more pharmacogenetic variants. Pharmacogenetic variants are those that influence how people respond to specific medications. This genetic data are being integrated into Marshfield Clinics EHR and decision support tools are being developed so our patients can receive the right drug, at the right time, and at the right dose. This project is supported by generous patient donations, financial support from Security Health Plan, and grant awards from National Institute of Health.

Marshfield Clinic Research Institute is the lead site in Wisconsin for thenationalAll of Us Research Program with collaborators at the Universityof WisconsinSchool of Medicine& Public Health, Froedtert &the Medical College of Wisconsin, and the Versiti Wisconsin, Inc. This study is a momentous effort to advance individualized prevention, treatment and care for people of all backgrounds. Murray Brilliant, PhD, Interim CPMR Director, is leading the state-wide recruitment efforts. The catchment area of the Wisconsin consortium covers 80% of the state thereby reflecting the true diversity of the state including both rural and urban populations.For additional information, including how to participate, visit JoinAllofUs.org, email allofus@marshfieldresearch.org, or call (888) 633-9987.

The mission of the Center forPrecision Medicine Researchis to conduct translational research in medical genetics that substantially improves patient care. Dr. Murray Brilliant affirms that the Center is committed to advancing scientific knowledge through humangenetics research and to translating that knowledge into practical applications that will foster improved health.

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The Marino Center for Integrative Health – Cambridge – Mount …

Posted: March 9, 2019 at 6:49 am

At the Marino Center for Integrative Health, we're honored that you have put your trust in us to deliver the best in comprehensive healthcare services. Here, we offer a unique, integrative model of healthcare, featuring both conventional primary care and a full array of complementary services - conveniently brought together under one roof - to promote your health and the pursuit of a healthy lifestyle.

Integrative Health - its in our name, but you may be wondering exactly what it means. Well, for us at the Marino Center, Integrative Health, or Integrative Medicine, means a unique model of healthcare focused on treatingyou as anindividual, not the disease. Here we aim to restore the focus on health and healing through a strong patient/practitioner relationship. For us, it also means taking more time to understand you and your needs, making sureyou feel listened to, and building an individualized plan for living better and staying better.

With a broader approach to healing and wellness, Integrative Medicine takes into account all aspects of what makes a person whole - mind body and spirit and addresses them all at the same time. Through an active practitioner and patient partnership, a treatment plan including the use of both traditional (sometimes also referred to as conventional or western medicine) and alternative (also called CAM and complementary) therapies is developed to promote health AND prevent illness and disease.

Relying on the best of scientifically-validated conventional and alternative therapies, Integrative Medicine is a practical strategy that puts the whole patient at the center of care. In addition to physical symptoms, it examines the psychological, social and spiritual nature of the individual. An integrative approach educates and empowers the patient to play an active and responsible role in his or her own health.

Our mission is to integrate scientifically and empirically demonstrated conventional and complementary healing traditions to improve the health of those we serve and to extend our knowledge to others through health education, training, and research.

Patient Care

Providing healthcare services through a collaborative team of compassionate, innovative practitioners who provide preventive health care, and work with patients with acute and chronic conditions

Education and Training

Providing each person seeking our services with scientific explanations and informed choices regarding the professional care and self-care of body, mind, and spirit

Sponsoring and participating in public and professional presentations, seminars, and symposia along with publications and other media to educate the general public, medical, and insurance institutions about the unique potential of integrative medicine

Educating medical students through clinical rotations, internships, residencies, and fellowships

Research

Examining the applied clinical aspects of integrating complementary and alternative therapies to determine efficacy and safety

Participating in research studies on specific medical practices and our integrative model with leading research institutions

Performing critical analyses of the service delivery system and examine the clinical and administrative structure of integrative medicine

Lelio 'Les' Marino was the visionary, founder and generous benefactor of what was then known as the Marino Foundation for Integrative Medicine, the sponsor of The Marino Centers for Progressive Health. Because of his vision and generosity, the Center has grown into one of the region's premier providers of integrative medicine and healthcare services.

Our origins date back to 1993 when Mr. Marino acquired a small alternative medical practice in Cambridge, MA. From this humble beginning, he envisioned a model of care that would integrate mind, body, and spirit, and genuinely blend traditional and complementary healing practices. In 2008, we integrated our two names and became the Marino Center for Integrative Health. Throughout the years, the Marino Center has been guided by Mr. Marino's vision and its mission of providing patient care, education, and research - and continues to be today.

After a decade operating centers in Cambridge and Wellesley, in 2013, the Marino Foundation for Integrative Medicine decided to turn its focus to supporting new initiatives in Integrative Medicine, and the Cambridge Marino Center for Integrative Health was acquired by Mount Auburn Hospital, a Harvard-affiliated teaching hospital with a long history of support for integrative, alternative and prevention-based medical approaches.

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Stem Cell Lake Havasu City Arizona 86406

Posted: March 8, 2019 at 6:44 am

Stem cell therapy has actually ended up being a popular dispute in the global medical scene. This highly questionable therapy has received combined opinions from numerous stakeholders in the healthcare industry and has also attracted the attention of political leaders, religious leaders and the basic population at large. Stem cell treatment is considered a revolutionary treatment for people struggling with a vast array of degenerative conditions. Some typical questions concerning this therapy are responded to listed below.

Are you a stem cell therapy specialist near Lake Havasu City AZ 86406? Contact us for more information about joining our website.

Stem cells can be described as blank state or non-specialized cells that have the capability to become specialized cells in the body such as bone, muscle, nerve or organ cells. This implies that these special cells can be utilized to regrow or develop a wide range of damaged cells and tissues in the body. Stem cell therapy is for that reason a treatment that aims at attaining tissue regrowth and can be used to treat health conditions and illnesses such as osteoarthritis, degenerative disc illness, spinal cord injury, muscular degeneration, motor neuron illness, ALS, Parkinsons, heart disease and many more.

Being a treatment that is still under research study, stem cell treatment has actually not been fully accepted as a viable treatment choice for the above discussed health conditions and illnesses. A lot of research study is presently being performed by scientists and medical specialists in numerous parts of the world to make this treatment practical and reliable. There are however numerous restrictions enforced by governments on research involving embryonic stem cells.

Presently, there havent been numerous case studies performed for this form of treatment. Nevertheless, with the few case studies that have actually been carried out, among the significant issues that has actually been raised is the increase in a patients risk of developing cancer. Cancer is brought on by the fast multiplication of cells that tend not to pass away so easily. Stem cells have actually been associated with similar growth elements that may lead to formation of tumors and other malignant cells in clients.

Stem cells can be extracted from a young embryo after conception. These stem cells are commonly described as embryonic stem cells. After the stem cells are extracted from the embryo, the embryo is ended. This is basically among the major reasons for debate in the field of stem cell research. Many individuals suggest that termination of an embryo is unethical and undesirable.

Stem cells can still be acquired through other means as they can be found in the blood, bone marrow and umbilical cables of adult humans. Typical body cells can likewise be reverse-engineered to become stem cells that have limited capabilities.

New studio has nevertheless revealed promise as researchers target at establishing stem cells that do not form into tumors in later treatment phases. These stem cells can therefore efficiently change into other kinds of specialized cells. This treatment is for that reason worth investigating into as numerous patients can benefit from this revolutionary treatment.

The best stem cell provider near Lake Havasu City AZ 86406

4.857

Main address:Lake Havasu City, Arizona, 86406

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