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What To Expect With Testosterone Shots – Mantality Health

Posted: July 7, 2018 at 8:42 pm

What To Expect With Injectable TestosteroneDeciding to treat low T with injectable testosterone is the first step to a healthier life. Heres what you can expect from testosterone shots.Keyword(s): injectable testosterone

Most peopleknow what testosterone isa male steroid hormone. We associate it with manliness.

But testosterone does more than just promote a healthy sex drive. It also affects body fat, bone density, muscle mass, red blood cell count, and mood.

The normal testosterone levels in men arebetween 300 and 1,000 ng/dL.

But if you get ablood test that shows your levels are far below that, your doctor may suggest testosterone replacement therapy in the form of injectable testosterone.

While its totally normal for men to startlosing some of their testosterone when they hit their 30s or 40s, more rapid declines in testosterone levels couldindicate a more serious problem called low T.

Common symptoms of low T include:

Some men may also notice shrinkagein the size of their penis and testicles, while others may haveswelling of the breasts. These symptoms can take many months before you notice them.

Its at this point that you may consider testosterone replacement therapy.

The injection site is most oftenin the gluteal muscles in the buttocks. But ifyour doctor allows you to do the injections yourself, the injection site would be in your thigh.

Whatever the case, if your doctor recommends injectable testosterone, you need to be realistic.You are not going to drop 25 pounds in a monthor quickly add 2inches.

Thats just not how it works.

The benefits of testosterone therapy will not take years though. It will take a longer amount of time to see some benefits than others as theincreased testosterone starts working on a cellular level.

But if you go into this therapy with realistic expectations, youll probably be pretty satisfied with the changes you eventually see.

In general, you can expect to see results within thefollowing time frames:

On a cellular level, there will be improved insulin sensitivity. Although this improves within the first few days, you may not notice the effects for 3-12 months.

So this is the time where youll probably start to notice some real differences.

Most men report an improvement insex drive, sexual thoughts, fantasy, satisfaction, and libido. There is also an increase in morning erections and ejaculation

At this stage, many men also seea decline in anxiety and aggression. They tend to feel more sociable, awake and attentive.Some report feeling more creative.

A reduction in inflammation typically begins around this third week too, and continuing to lessen throughoutthe remainder of treatment.

Improved erectile function begins around this time and continues to improve over the next several months. Mental alertness and improved memoryare alsoapparent during this time with continued improvement expected.

A slight decrease in triglycerides and total cholesterol begin, while an increase in full erections and sexualperformance are noticeable.

Feelings of depression and lack of motivation begin to lift around the six-week point, althoughthe maximum benefitstake longer to take effect.

In most cases, men report a general satisfaction with their sex lives by the end of the eighth week.

This is the time when youll start to notice a change in lean body mass, fat mass and muscle strength.

There will be an improvement in glycemic control and the formation of red blood cells (known as erythropoiesis) and a decline in blood pressure.

The decreases in LDL cholesterol levels and increases in HDL cholesterol levels continue, though the maximum changes typically occur some time between 6 and 12 months. (Proper diet and exercise play into this too.)

Additionally, there is an increased exercise capacity at this point even for men who experience chronic heart failure.

As mentioned above, many of the benefits are continuing to maximize over the course of your treatment.

At the six-month point, there is increased bone density, as well as a rise in the prostate-specific antigen (PSA) levels which are typically very low in those with low T.

The list below gives you the overall benefits from using this testosterone replacement therapy.

Keep in mind though that while injected testosteronecan help regulate fat distribution, youlikely wont experience seriousweight changes from hormone therapy alone.

As for maintaining muscle, it should be noted that injectable testosterone and other therapies have been found to help increase muscle mass, but not strength.

Still, the results from injectable testosterone can be life changing. Especially for those who experiencedebilitating symptoms associated with low T.

If you makethe decision to try testosterone therapy, youll want to be educated on what will happen once the treatment ends. Fortunately, you can turn to hormone replacement therapy specialists who can guide you to ensure that your body continues to produce enough testosterone.

Specific medication protocols are usually followed at the end of treatment so that the effects of low T dont return.

While its not a cure, using injectable testosterone is an effective therapyto help you reach alevel of wellness and vitality thats closer to what you felt when you were younger. And this will give you a better quality of life.

If you have already experienced the benefits or drawbacks of injectable testosterone therapy, please feel free to share below.There are some guys out there who could benefit from your experience.

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What To Expect With Testosterone Shots - Mantality Health

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HCG Diet Food List – HCG Diet

Posted: July 7, 2018 at 8:41 pm

Basics of HCG Approved Food List

While on the HCG diet, Dr. Simeons explains in his protocol that a strict diet of 500 calories a day is necessary in order to see the full effects of the HCG hormone. Within his protocol Pounds and Inches Away published in 1967, Simeons explains that The diet used in conjunction with HCG must not exceed 500 calories per day, and the way these calories are made up is of utmost importance. Simeons knew that simply cutting calories, was not the proper method to weight loss due to the harmful effects certain foods could still have on the body. This methodology is emphasized as he states, if a patient drops the apple and eats an extra breadstick instead, he will not be consuming more calories, but he will not lose weight. This is due to the fact that certain foods, especially fruits and vegetables, could have low caloric value, but are absent from his list due to low nutritional value. These foods can interfere with regular weight loss while on the HCG diet, due to their composition. Simeons points out pimiento peppers, okra, artichokes and pears as examples of foods with few calories, but are still harmful to the body when trying to lose weight.

Something to keep in mind when on the HCG diet is your geographic location. Simeons, who wrote his protocol in Italy, based off of the caloric value of Italian foods, explains that certain modifications should be made if you are dieting in another country. For example, beef in the United States has almost double the calories of South Italian beef, which contains much less fat. Because of this, Simeons substitutes low-grade veal to be used while following the diet. Italian breadsticks, known as Grissini, are perfect for the HCG diet, but are mostly unavailable to people in the U.S. Melba toast can be used instead, even though, as Simeons puts it they are psychologically less satisfying. Even when changes are being made to the diet due to location-based feeding habits, Simeons continues to stress that the total daily intake should not exceed 500 calories. If dieters would like to see the best results, they should follow this protocol, sticking to approved foods. A daily ration should contain 200 grams of fat-free protein, a mix of fruits and vegetables, and a small amount of starch.

In his protocol, Simeons also makes it clear that because the daily dose of HCG drops is consistent for everyone, the same diet should be followed, regardless of who is doing it. The same diet should prove to be effective whether it is a small elderly lady of leisure or a hard working muscular giant. Simeons states that no matter the shape or size of the body, it is able to get all the calories it needs from abnormal fat deposits, regardless of whether you are burning 1,500 or 4,000 calories per day. Simeons emphasizes this point in his protocol, explaining that we use our fat for fuel far more often than we use what we eat to move us during the day. Before diving into the specific foods allowed on the HCG diet, Simeons breaks down a few unique scenarios that could apply to a variety of people trying to follow the HCG diet.

The first example he covers is vegetarians, who are unable to consume any sort of animal protein. In order to obtain the proper amount of protein per day, which is 200 grams, Simeons says they must drink 500 cc or just over 2 cups of skimmed milk per day. When it comes to fruit, vegetables and starch, the vegetarian diet is very similar to that of the HCG diet. However, they will not consume their usual intake of vegetable proteins from legumes such as beans or derived from wheat and nuts. Due to some of these restrictions, vegetarians see about half the average weight loss of non-vegetarians while following this plan. Simeons attributes this to the amount of sugar in the milk that vegetarians need to substitute for animal protein. More information about following the HCG Diet as a vegetarian can be found here.

Faulty dieting is another aspect of the HCG diet that Simeons stresses in his protocol. While on the HCG diet, it is crucial that the approved foods list is followed, as the slightest deviation from the diet has disastrous results as far as the weight is concerned. While taking the HCG hormone and following the strict diet, the body is extremely sensitive to anything that isnt approved by Dr. Simeons list. Small errors can stop the weight loss process, and ruin previous progress. Simeons mentions that people in high official positions are still able to follow the HCG diet with the proper preparations and discipline. If they are at official dinners, it is important to stick to the diet, and work around foods that dont follow the guidelines. This is extremely important due to the fact that a meal against protocol can cost them three days of treatment progress. Something that many patients have questioned about the HCG diet is the use of vitamins. There is a common fear that the restricted diet is limiting the amount of vitamins and minerals they are getting. However, physicians can assure their patients that when they are losing fatty tissue, only the actual fat is being burned, and the vitamins, proteins and minerals contained within the foods are still taken in by the body.

Before going over the list of approved foods, it is important to understand the specific way in which the diet should be followed. Every item on Dr. Simeons list is carefully researched and picked for specific reasons. No variations or substitutes can be used for the approved food list. All items that are not on the list are forbidden, due to the fact that nothing permissible has been left off. When it comes to the daily ration of meat, the 200 grams should be weighed raw, after any visible fat has been trimmed off. Another important aspect Simeons points out is the way in which meals are broken up. A breadstick and an apple can be eaten for breakfast or right before bed, as long as it is deducted from the regular meals. However, it is important to keep in mind that a daily ration of two breadsticks and two fruits should not be consumed at the same time. Furthermore, items saved or not eaten in previous days cannot be transferred and eaten on the next day. Since a new diet can often be difficult to track at first, Simeons highly recommends keeping a diet sheet to reference, with a list of everything consumed each day. If a cheat does occur, find out what you can do about it here. Some patients have asked before about following the diet protocol without taking the HCG hormone. Simeons is very much against doing this, and explains that it will lead to trouble in just a few days. Furthermore, it is important to note that showing off to friends about your dieting powers, without letting them know about taking HCG hormones is also dangerous. This can cause friends to try the same diet without the hormone, which proves to be a failure and can often be dangerous.

When it comes to finding loopholes in the diet and protocol, Simeons suggests finding another way to lose weight instead. His protocol and plan was researched for decades, and each food and procedure is chosen for a specific reason. Loopholes such as eating two small apples instead of the recommended one large apple, can change the caloric value delivered and alter dieting results. Similarly, chicken breast must be from a chicken and cannot be replaced by the breast of any other fowl. Simeons promises results if dieters follow the exact process and protocol, and any attempts at tricking the system or getting around certain foods can prove to be detrimental to results. It is best to follow the process that he lays out in his manual, and follow this list of approved foods, straight from his book Pounds and Inches Away. With each of Dr. Simeons approved foods, we will also dive into the benefits of consuming each when it comes to not only weight loss, but also overall vitamin, mineral and protein content.

Beginning with breakfast, tea or coffee in any quantity is allowed, but no sugar can be added. Instead, a sugar substitute should be used, such as Trulicious Natural Sweetener. A tablespoon of milk is allowed per 24 hours. As for lunch, there are a variety of meat options that are approved by Dr. Simeons. These include 100 grams of veal, beef, chicken breast, fresh white fish, lobster, crab or shrimp. Before cooking all meats, they should be trimmed of all fat and weighed raw. The meat should then be boiled or grilled, with no additional fats added. Any fish such as salmon, eel, tuna, herring, dried or pickled fish are not allowed on the HCG diet. White fish such as Tilapia, Cod and Halibut are permitted, as well as albacore tuna (packed in water only).

Along with your meat choice at lunch, one type of vegetable can be eaten. Most vegetables are approved for the HCG Diet, with the exception of high starch options such as corn and potatoes which are left off the list. All greens are approved on the diet and eating a variety of vegetables each day is highly recommended. The protocol states that 2 cups of vegetables per day should be eaten, and split between both meals. Dieters should choose from the following list: spinach, chard, chicory, beet-greens, green salad, tomatoes, celery, fennel, onions, red radishes, cucumbers, asparagus and cabbage. One breadstick (Grissini), or if not available, one Melba toast can be eaten as well. For fruits, one apple, a handful of strawberries or one-half grapefruit rounds out the meal. The fruit or breadstick can be eaten between meals, instead of during lunch or dinner, however, no more than four items on the list should be eaten at one meal. As for dinner, the same four choices should be used as lunch.

Simeons also outlines a variety of rules that go along with what can be eaten while on the diet. Tea, coffee, plain water, or mineral water are the only drinks that can be used, however, they can be consumed in any quantity, at any time. Check out these 6 beverages you should be drinking when on the HCG Diet. Simeons recommends that HCG dieters consume around 2 liters of fluids per day. Many dieters believe this will cause them to retain more water, however, this is not the case, and furthermore, the body is inclined to store more water when it is not being hydrated enough. The juice of one lemon per day is allowed for any purpose. As for seasoning, salt, pepper, vinegar, mustard powder, garlic, sweet basil, parsley, thyme, marjoram, etc. can all be used. However, no oil, butter or dressing should be consumed while on the diet.

With most diets, there are always variations to what the proper foods and processes are to lose weight. The HCG diet has had a resurgence, due to Dr. Oz bringing it to light in 2013. Dr. Ozs vegetable list contains: any kind of lettuce, onions, tomatoes, fennel, cucumber, radishes, celery and asparagus. For proteins he lists white fish, lobster, crab, shrimp and extra lean beef (96% lean ground beef including pot roast). For fruits his list contains apples, blueberries, blackberries, strawberries and lemons. For condiments, Dr. Oz approves Braggs Liquid Amino (healthy soy sauce substitute), mustard, apple cider vinegar and any natural herbs or spices. While not drastically different than Simeons approved foods, it is important to note that there are a variety of methods and processes you can follow while on the HCG Diet. We suggest following the approved foods list that is explained in the previous paragraphs, which come straight from Dr. Simeons book, Pounds and Inches Away.

When it comes to salt, Simeons explains that there is no restriction on the use of salt. Instead, he focuses the attention on hydration and ensuring that dieters drink large quantities of water while taking the HCG hormone. The goal of reducing abnormal fat is a completely separate process than salt consumption, and does nothing to affect weight loss. However, although there is no limit, the daily salt intake should roughly stay consistent as not to suddenly alter the bodys balances, which can give faulty results day to day.

Another important aspect of the HCG diet, often overlooked, is the use of cosmetics, which can often be a hidden issue when it comes to weight loss results. Simeons explains that many of his patients were surprised to hear that the fats and oils contained in their creams and ointments could be absorbed after being applied to the skin. Once absorbed, they can interfere with weight loss, in the same way as if the fat and oil had been eaten. Simeons emphasizes the sensitivity of the body while doing the HCG diet and the importance of controlling as many variables as possible. This minor increase in fat and oil can have dramatic effects on the success of the diet. Simeons uses an example of a worker at a beauty parlor, who is constantly handling organic fats, and never had a satisfactory loss of weight until they eliminated contact with these fats and their skin. Simeons solution is to use plain mineral oil, which has no nutritional value. However, mineral oil should not be used in preparing food, since it has laxative qualities and can absorb fat-soluble vitamins which are later released from the body. The use of lipstick, powder and lotions that are free of fatty substances, are all approved on the HCG diet. Brilliantine can also be used on the hair, but not to be rubbed into the scalp. Sun-tan oil is strictly prohibited. Approved cosmetics include: Mineral based/oil free foundation/lotions, bare essentials/bare minerals, mineral stick deodorant (Crysalux Crystal), baking soda, face soap/make up removers, Arbonne, Neutrogena Oil Free Cream Cleanser, and Witch Hazel. For skin and lip moisturizers, there are a variety of approved products to choose from. However, various foods can also be eaten to promote healthy skin, check out the link for more info. For HCG approved products they include: Alba Oil Free Facial Moisturizer, Aloe Vera 100%, coconut oil (cold pressed), Fiji Organic Virgin Coconut Oil, Kiss my Face Oil Free Moisturizer, St. Ives Vanilla Lotion. As for shampoos and conditioners, approved products include Aubrey Organics, Life Extension, Magik Botanicals Oil Free Shampoo and Conditioner, Mastey Products, and Natures Gate Alovera. Dial soap can be used as well as baking soda for everyday cleaning. Sunscreen should be used based off the following products: Aveeno Oil Free Sunscreen, Clarins Oil Free Sun Care Spray SPF 15, Coppertone Oil Free Sun Block for Faces SPF 30, Coppertone Oil Free Sun Block Lotion, Ocean Potion Sport Xtreme Sun Block Water and Sweat, Oil Free Sport Xtreme Sun Block, Peter Thomas Roth Ultra Lite Oil Free sun products, Zia Oil Free Sunscreen SPF 15. For toothpaste, Spry and Toms of Maine toothpaste are both acceptable. For an easily printable list, check out a List of Recommended Beauty Products Safe for HCG.

To aid dieters in the choosing of foods on a daily basis, we will break down the caloric information of the approved foods, explain the various benefits you receive from each item, and provide a few HCG approved recipes that you can try out at home. This will help you better plan out what foods youd like to eat, and stick to your diet goals.

Fish (3.5 oz):

Meat (3.5 oz):

Vegetables (3.5 oz):

Fruits:

Bread

Example meal plans can be found below, along with the overall health, nutritional and weight loss benefits that each food provides. For an easily printable list of approved foods, check out this link.

Breakfast: Herbal tea with Trulicious Natural Sweetener, and 1 piece of Melba Toast

Lunch: 3 oz of grilled chicken breast, 3.5 oz of Asparagus, 1 breadstick and a small apple

Dinner: 3 oz of baked halibut, 3.5 oz cauliflower, 1 breadstick and 12 strawberries

Nutrition Facts: 494 calories, 10.6g fiber, 31 carbs, 51g protein, 229mg sodium

Starting your day with a cup of herbal tea such as black tea or green tea is a great choice when it comes to your overall health. Green tea specifically has been shown to increase fat burning and boost your metabolic rate. In a study done on 10 healthy men, it was found that green tea increased energy expenditure by 4%. More information about this study can be found here. Mix in a spoonful or two of substitute sugar, such as Trulicious Natural Sweetener, which is 3 times sweeter than sugar, but has very few calories and a low glycemic index. To go along with your tea, a slice of Melba Toast is an excellent way to incorporate a good source of fiber and complex carbohydrate into your diet.

Following breakfast, the caloric intake increases, with four items on the menu. At over 20g of protein and only 200 calories, grilled chicken breast is a great way to give your body the protein it needs to keep your muscles strong and well-fed while working out. Made up of important amino acids, the protein contained in chicken helps to form the building blocks of muscles in the body. Not only is chicken great for its protein content, but also the vitamins and minerals it contains. For example, the Vitamin B that chicken provides, can help boost immunity, regulate digestion and prevent problems such as migraines, high cholesterol and diabetes. Also contained within chicken is vitamin D which helps the body absorb calcium and strengthens bones. Vitamin A, also found in chicken, helps with overall eye health, while its traces of iron help with muscle activity. Overall, diets with high levels of protein are shown to help with weight loss, as it provides the necessary protein your muscles need to burn abnormal fat. Along with the chicken at lunch, choose a few ounces of asparagus. Its packed with fiber and antioxidants that keep your body running smoothly. Vitamins such as A, C, E, and B6 as well as high levels of iron, copper, and calcium, give your body the key nutrients it needs while on a restricted caloric intake. At only 32 calories per cup, asparagus contains a lot of soluble and insoluble fiber, which is great when youre trying to lose weight. The fiber, while helping with digestion, is consumed slowly by the body and keeps you full longer in between meals. Antioxidants found in asparagus, especially the purple kind, can help your body fight against cancer cells and strengthen your immune system. Adding a bit of sweetness to the end of your meal can be a great way to boost your energy, while also providing your body with excellent nutrients and vitamins. Apples, ranked first in Medical News Todays article on top 10 healthy foods, are high in Vitamin C, a powerful natural antioxidant that can fight against free radicals and infectious agents. Along with minerals such as calcium, potassium and phosphorus, the B-complex vitamins within apples are great for maintaining red blood cells and keeping the nervous system in great shape.

Dinner contains plenty of natural and nutritious items such as halibut, cauliflower and strawberries, all of which have immense health benefits to the body. Halibut is an excellent source of protein, potassium, selenium and vitamin B12. It is also rich in fiber, and contains more than oatmeal and a variety of vegetables. The Satiety Index of Common Foods also ranks white fish such as halibut, as number two for the most filling foods, only topped by potatoes. This is due to the high protein content and also the influence it has on serotonin, a key hormone responsible for controlling appetite signals. Omega 3 fatty acids, which can be found in various white fish, are essential for the body and cannot be synthetically created. Therefore, it needs to be supplied from an external source such as cod and halibut. Other nutrients such as magnesium, in which halibut contains 22mg, helps relax arteries and veins in the body, which in turn, increases blood flow. Cooking methods such as broiling, baking and grilling, instead of deep frying, can help keep all the nutrients in the foods.

Pair your halibut with some steamed or baked cauliflower which is rich in vitamins and minerals. Cauliflower contains high levels of sulforaphane, a sulfur compound that has been seen to kill cancer stem cells and limit tumor growth. Its anti-cancer effects can also help prevent prostate cancer, when combined with turmeric, which contains the active compound curcumin. Not only is it great for preventing cancer, but it also controls inflammation in the body due to its various compounds. Lastly, cauliflower contains 77% of the recommended daily value of vitamin C, which is key to body development and repairs of body tissue. Also a good source of vitamin k, thiamin, phosphorous and potassium, cauliflower is a one-stop shop for many vitamins and minerals your body needs to thrive. Finishing your meal off with a dozen strawberries is a great way to not only satisfy your sweet tooth, but also provide your body with vitamins, potassium and fiber. All of these can help with weight loss and strengthen your body overall. Strawberries help promote the creation of adiponectin and leptin hormones, which both help with fat burning and increasing your metabolism. They also have anti-inflammatory properties by limiting the impact of enzymes and genes that can promote inflammation in the body. This is due to the high antioxidant content contained within them. Eating water rich foods such as strawberries, cucumbers and tomatoes, which can contain up to 90% water, helps contribute to better overall hydration if eaten with a steady consumption of water as well. Containing more vitamin C than oranges by weight, one cup of strawberries fulfills 141% of your daily vitamin C needs. While not directly shown to cause weight loss, vitamin C deficiencies have been shown to hinder weight and body fat loss, so ensure that youre fulfilling your daily needs while dieting. Vitamin C has also been shown to help dieters with high blood pressure. Lastly, strawberries are rich in nitrate, which can help increase the flow of blood and oxygen to your muscles by 7%. This has been shown to help dieters burn more calories and lessen overall fatigue.

Breakfast: Herbal tea with Trulicious Natural Sweetener, and 1 breadstick

Lunch: 3.5 oz of shrimp, 3.5 oz spinach, 2 Melba Toast and a small orange

Dinner: 3 oz of beef, 3.5 oz tomatoes, 1 breadsticks and grapefruit

Nutrition Facts: 493 calories, 19g sugar, 46 carbs, 45g protein, 281 sodium

Shrimp packs a punch when it comes to key nutrients and protein content. At around 20 grams of protein per 3 ounces, it provides just a few grams less than the same amount of chicken. A single jumbo shrimp provides about 3 grams of protein, but has very little fat and carbohydrates, making it a great food while trying to lose weight. It also comes close to fulfilling the bodys daily value for selenium, a trace mineral that can help limit inflammation, protect against heart disease and maintain a healthy metabolism. Along with selenium, shrimp provides around 60% of your daily vitamin B12 needs and 40% for phosphorous. To go along with the shrimp, try some sauteed spinach, which provides your body with 3.6g of carbohydrates, 2.2g of which are dietary fiber. Spinach contains 70% insoluble fiber, which helps improves digestion function and the transportation of food through the body. The other 30% is soluble fiber, which keeps your body from absorbing some fats contained in other foods. Fiber plays an important role in the body and especially weight loss. Learn more about the different types of fiber here. Spinach also has a low glycemic index of just 15, which makes it the perfect food for promoting weight loss and eliminating cellulite. 100 grams of spinach also contains 558mg of potassium, about 16% of your daily value. Containing more potassium than 100g of bananas, increased consumption helps with preserving bone mineral density and retaining muscle mass. It has also been shown to help prevent kidney stones, as well as control heart rate and blood pressure. To finish off lunch, try a small or medium orange. They have over 170 different phytochemicals and 60 flavonoids, many of which have strong antioxidant benefits and anti-inflammatory properties. One orange provides 130% of your daily vitamin C needs, which helps boost your immune system and keeps the body running smoothly. Choline, a vitamin contained in oranges, with 36mg per orange, has been shown to help with sleep, muscle movement, learning and memory.

For dinner, the main meal of beef, tomatoes and grapefruit, contains a variety of

vitamins and minerals that our bodies need. At around 26 grams of protein, the 100g of beef will keep you full and strong while on the HCG diet. Beef also provides 35% of your vitamin B3 needs and 107% for vitamin B12. Maintaining a strong supply of proteins, vitamins and minerals is crucial while on the HCG diet, due to the decrease in calories consumed. It is important to provide your body with the building blocks it needs to stay healthy as you lose weight. To go along with your main source of protein, tomatoes are a superfood when it comes to nutritional content and the benefits it provides your body. They are rich in vitamin C, at around 40% of your daily requirement per tomato, and also in vitamin A, a powerful antioxidant that can improve your vision. Lycopene, a component of tomatoes, has been proven to help protect against cardiovascular disease and decrease cholesterol levels. Finishing the meal with half a grapefruit, brings the body similar benefits to the oranges consumed at lunch, due to its high vitamin A and C levels. However, it still has a low glycemic index of 25, therefore, not significantly affecting blood sugar and insulin levels. Studies done by Dr. Ken Fujioka as part of the Scripps Clinic, showed that on average, obese participants who consumed fresh grapefruit before each meal, lost the most weight at 3.52 pounds over 12 weeks, with the placebo group only losing 0.66 pounds. Along with its weight loss benefits, grapefruits are one of the most hydrating fruits on the planet, made up of 91% water, and providing the body with important electrolytes.

Tasty, easy to make, and HCG approved recipes for the foods above can be found in the links below:

More HCG approved recipes can also be found here!

Simeons notes in his protocol that there is no objection to breaking up the two meals. For example, there is the option to have a breadstick and an apple for breakfast or before bed, as long as it is removed from the regular meals and no more than four items are eaten during one meal. When first following the HCG diet and planning out meals, Dr. Simeons suggests keeping a diet log, in order to properly measure caloric intake and food consumption. Relying on memory can be difficult, and preparation will always serve to keep dieters organized and on-track with their diet needs. Overall, sticking to a plan is the most important aspect of the HCG diet and following the careful way in which the foods, process and treatment was designed.

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HCG Diet Food List - HCG Diet

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The Real HCG Diet site | Buy the Web’s only Non …

Posted: July 7, 2018 at 8:41 pm

The Real HCG Diet site | The Webs Only Non-Homeopathic HCG Drops Supplier 2018. All Rights Reserved.

Disclaimer: Results discussed on the website are not typical. Information provided herein has not been reviewed by the Food & Drug Administration and is not intended to replace or circumvent the advice of your personal physician or health care professional. No claim or opinion on this website is intended to be, or should be construed to be, medical advice or a substitute for professional medical advice. No intent to diagnose, treat, cure or prevent disease is implied or intended. We make no claims about specific products and any information contained herein is personal opinion only and should not be construed as medical advice in any way. The information contained on this website is provided for information purposes only. No information or opinion contained herein is intended nor implied to be a substitute for professional medical advice relative to your specific medical condition. You should always speak with a health care professional before taking or using any dietary, nutritional, weight loss, herbal supplement or any other product. We recommend you visit your doctor and discuss your health needs before beginning any diet or exercise program. Views and experiences notated here are not typical, but based on the protocol of Dr. A.T.W. Simeons, MD. Any and all forum discussions will default to Dr. Simeons HCG + very low calorie protocol.

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The Real HCG Diet site | Buy the Web's only Non ...

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The Stem Cell Institute of Texas | San Antonio, TX

Posted: July 6, 2018 at 5:51 pm

Welcome to the Stem Cell Institute of Texas

The Stem Cell Institute of Texas provides the latest stem cell therapies using adult stem cells to treat a wide variety of health concerns. Our two main focuses cosmetics and orthopaedic conditions use adult stem cells to transform your appearance and function by stimulating the bodys repair mechanisms.

Cells derived from the Umbilical Cord of Live Healthy Birth Babies contains stem cells, growth factors, and proteins that stimulate YOUR body to regenerate damaged joints and tissue Just like in your youth!

Do you suffer from pain in your back or your joints, such as hip or knee, which prevents you from living life to the fullest? Is walking, cycling, gardening, fishing, or exercising, no longer possible with out pain? If you are missing out on your Golden Years, you need to see a regenerative medicine physician for a consultation. They can determine if the newest innovations in stem cell therapies are right for you.

The treatment is a simple non-surgical injection into the affected joint with no down time or lengthy recovery. You may maintain your normal lifestyle and allow the cells to create a balanced optimal environment in your joints so your body can repair itself. You should feel maximal results within 10 to 12 weeks at which time you can increase your activity levels to match your comfort, but understand that the regenerative process can continue for long periods of time.

Umbilical cord cells contain growth factors, proteins, and stem cells that continue to produce additional growth factors and proteins for a period of time. These components have the potential to positively affect the environment inside the joint and to stimulate your own tissue to aid in the regenerative process while ALSO stimulating your NATIVE STEM CELLS to aid in regeneration.

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The Stem Cell Institute of Texas | San Antonio, TX

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Hcg Diet Plan: Get to Know the REAL Hcg Diet | HCG DIET …

Posted: July 6, 2018 at 5:51 pm

What is the Hcg Diet Plan? (And how to do it right.)

The Hcg Diet Plan is a doctor-supervised, medical weight loss program that combines hormone therapy with whole food eating. Menu options are heavy in vegetables and lean proteins, as well as fruit options and limited grains. Allowed calories on the protocol, range from 500 to 1600 calories, depending on the specific protocol followed.

The very specific food list can teach healthy eating, portion control and establish healthy relationships with food. While the protocol was initially outlined by Dr. Simeons, modern day variations have become increasingly popular. This guide, focuses on the original protocol, with modern adaptations noted by the editor.

How the diet started

Dr. Albert Simeons was a world-renowned endocrinologist recognized for his pioneering work in tropical diseases. He was awarded the Red Cross Order of Merit for his advancement in the research and treatment of Malaria and honored for a number of research-breakthroughs. While working with severely malnourished women in India, Dr. Simeons discovered a link between the Hcg hormone, and its ability to utilize stored fat to nourish and deliver healthy children. After years of research, he was able to develop a protocol that utilized this effect of Hcg, to treat obesity, and published the groundbreaking manuscript, Pounds and Inches.

Here are a few basics for those who are ready to jump right in and start with the plan immediately. With these simple articles, you can easily get started today. Take note however, that anyone new to the plan, should read through this guide thoroughly, and NEVER proceed with the protocol without consulting a physician (FDA approved telemedicine Hcg programs are listed below.)

The Original Hcg Diet Protocol Concise summary of the original protocol with tips and guidelines.

Original Hcg Diet Manuscript: Pounds and Inches The original manuscript by Dr. Simeons.

Hcg Diet Foods Lists What you can eat on the Hcg Diet (Phase 2 and Phase 3)

The Hcg Diet Phases

Hcg Diet Results

Success Stories with Before and After Pics

Buying Hcg Kits and Doctor-supervised Hcg Programs online

The Hcg Diet is a medical weight loss plan, and should ONLY be prescribed and overseen by a licensed medical doctor. While it can be challenging to find a local physician that specializes in Hcg hormone therapy, there are a few options available online, through FDA-approved telemedicine companies in the USA. These sources provide complete prescription Hcg kits for injections, drops and tablets/pills/pellets, and provide expert guidance throughout the duration of treatment.

Where to Buy Prescription Hcg Injections Online: Kits and Programs ( USA, FDA-approved sources)

Hcg Diet Drops Buying REAL Hcg drops vs. fake drops

Preparation

Preparing for the plan, is absolutely essential and should not be skipped. Much like building a house, you want a solid foundation and to be prepared for the steps and challenges that come along with the protocol. In fact, most challenges can be avoided completely by reading the following articles before getting started.

What is the Hcg Diet Plan?A thorough explanation of the Hcg Diet and how it works for weight loss.

Hcg Diet and Mindset

Hcg Diet Weight Loss Averages (Progress Averages)

Hcg Diet Stalls and Plateaus: Causes and Solutions

When to Stop the Hcg Diet Early and Regroup*

Setting Hcg Diet Goals

Hcg Diet Timeline and Calendar

Hcg Diet Journal and Tracker Spreadsheets

Resources

These tools can be beneficial for those starting out on the plan for the first time. They are all free to download. We will be adding more here, so please check back often.

Hcg Diet Resources (free downloads: tracksheet, manuscript PDF and Audiobook Mp3)

Help and Support: 3 Reliable Options

The Hcg protocol consists of 3 phases, with a newer 4th phase that has been added to the original protocol, being coined as P4, longterm maintenance. Each phase is extremely important and should not be skipped. The Loading Phase (P1) is 2 (in some cases, 3 days,) while Phase 2, the weight loss phase, can vary in length depending on your protocol's specifics. For example, one Hcg source's protocol can differ from another such as 26 days or 29 days. Phase 3, the Transition Phase, lasts 3 weeks and is an expansion of increased calories and foods that do not include any sugars or starches. The 4th phase refers to life after the diet's first 3 phases and includes a lifestyle of healthy choices learned while on the protocol. This includes exercise, portion control, clean eating and healthy living in general.

Hcg Diet Protocol Basics

Plans and Protocols(The original protocol vs. alternative and modern protocols.)

Hcg Diet Foods and Eating

Hcg Diet and Exercise

Hcg Diet and Medical Conditions*

The HCG Diet Protocol consists of separate phases. Together, they are referred to as a round. Dieter's typically lose 18 to 25 lbs per round (approximately 1 month.) If more weight is needed to be lost, there is a 3 week break after the final phase, and thenanother round is started. Rinse and repeat until goal weight is reached.

HCG Diet protocols include the following4 phases. Note on protocol variations: Some protocols actually include 3 and 4 as the same Maintenance phase and have only 3 phases total. Other protocols, including Dr. Simeons Original protocol, do not include phase 1, loading phase. This can be a personal preference, however we will say that the following phase example, has shown great results from our readers, and has certainly appeared to be the most successful.

1. Hcg Diet Phase 1 Loading

Phase 1 is the loading phase. Also known as the preparation phase. This phase is not on all HCG Diet protocols but it is on most and is very popular. During this two day phase, the dieter eats as much healthy fats as possible in preparation for starting the next phase. They are also welcome to eat as many cheat foods as they would like, with the intention of satisfying cravings prior to beginning the next phase where these foods will not be allowed. Read more

2. Hcg Diet Phase 2 Weight Loss

Phase 2 of the HCG Diet is the weight loss phase. This begins on day 3 of taking HCG where the dieter adheres toa specifiedcalorie intake and carefully selected food list. For most dieters, this is the most challenging phase of the plan because there is very little room for error. Read more:

3. Hcg Diet Phase 3 Transition

Phase 3 of the HCG Dietis at the end of HCG is discontinued and the same foods from phase 2 are still eaten, but increased slowly. In this stage, this is areintroduction period where calories and foods are slowly adjusted to maintain weight loss. Read more:

4. Hcg Diet Phase 4 Maintenance

Phase 4 of the HCG Diet is the phase of continuing weight maintenance for life-long health and fitness.This is an essential part of the HCG diet and it actually begins during Phase 2. This is where you will begin to build the skills necessary for lifetime weight maintenance and healthy living. For example; reading labels as a habit (this will become 2nd nature and shouldn'tstop once you reach your weight loss goals. Also, portion control and learning to listen to your body. Read more about these skills and implement them starting NOW:

The absolute best (&Free!) HCG Diet tips for reaching your weight loss goals safe and fast!

Hcg weight loss foods and recipes are the backbone of the Hcg weight loss program. Not only do the allowed foods help you to lose weight, but they also help get our body into a healthy state.

It is important to understand that while the Hcg hormone does NOT make you lose weight, it does changehowyour body loses weight, and creates a reaction in your body that allows for the rapid weight loss. It is the carefully selected Hcg Diet food list that is responsible for your weight loss; the Hcg just allows your body to continue to burn fat when it would normally go into starvation mode under the calorie restricted diet.

This is good news! This means the Hcg weight loss plan can be surprisingly versatile with the right recipes, and can work with any number of menus, foods sensitivities. The allowed foods are clear of processed and prepared foods and carefully measured for portion control. Instead of processed foods, the food list focuses on clean eating, and consists mostly of vegetables and healthy portions of lean protein, along with some, but limited, carbohydrates. To be clear, this is NOT a ketosis diet when done correctly, and in fact the amount of vegetables and fruit permitted, do not allow the body to go into ketosis. Compared to ketosis diets, this particular approach has been shown to average much higher weight loss rates that range from .5 to 1.5 pounds per day.

In a nutshell: The Hcg Diet allowed foods and guidelines focus on portion control, increased vegetables and proteins, and a food list that is comprised of clean, unprocessed foods.

The Original HCG Diet is known for its restrictive and unforgiving500 calorie VLCD (very low calorie diet.) Some versions of the diet have evolved to allowfor an HCG Diet expanded food list.

Learn what to eat on the HCG Diet and how to eat: Portion sizes, HCG Diet-friendly recipes for all phases, foods lists, plus HCG Diet cooking and shopping tips.

Learning to cook on the diet, can be a struggle for some patients, however, it is also an excellent opportunity to learn how to cook new foods that are clean, nutritious and healthy. Since the protocol does not allow foods that are processed or prepared (bye bye alcohol, fast food and frozen dinners) this can be an excellent path to learning how to prepare meals that will nourish your body, and make long term weight maintenance easy.

Take a look at our recipe section, and discover amazing Hcg Diet Recipes that include Southwestern chicken, strawberry cupcakes, garlic baked shrimp, mouth-watering grilled asparagus, and chai beverages. While it may be intimidating to look at the allowed foods list and feel limited, it won't take long before you realize just how versatile and delishous it can be.

We have also included a list of our best recipe and cooking articles that cover everything from how to cook on the protocol, spices to use to increase your metabolism, tips for dining out and tips for eating during special occasions.

Sample menus and menu tips for the HCG Diet.

How to Buy Hcg Injection Kits, Drops and Pellets

Hcg Diet Info has put together a complete guide to buying Hcg Kits and Programs online: How to Buy Hcg injections online (Drops and Pellets too.)

In the last few years, the FDA-approved telemedicine industry has grown and allowed doctors to prescribe and care for individuals wanting to follow the protocol. You can now safely buy Hcg kits online, that come with a complete program overseen by a medical doctor and Hcg weight loss experts and nutritionists. This has grown to be one of the most beneficial medical weight loss programs in the world now.

Tips for buying Hcg and Hcg weight loss programs: One of the first things to take into consideration when buying Hcg, is which form of Hcg you feel comfortable with, how soon you would like to start your diet, and the level of support you will need. The latter tends to be the biggest difference between various Hcg sources with some companies offering minimal support (better for veterans and pros that want to buy a bare bones Hcg kit to save money) and other companies offering extensive diet support, while yet another offering custom dosage and protocol support, and complete medical doctor hand-holding, along with a year of nutritionist services (even after the diet is completed.) These may seem like small differences but they can add up to be an extreme value for your money, as well as offer peace of mind.

The sources recommended in Hcg Diet Infooffer each of the acceptable choices that can be used with the original Hcg weight loss protocol: Hcg injections, Hcg Diet Drops (Prescription or Homeopathic), and Prescription Hcg Pellets. The Hcg Buy Guide also offers answers to dozens of frequently asked questions about buying Hcg Kits and Hcg weight loss programs online. Please note: Over the counter hormone free diet drops should NOT be used with the original protocol and can be dangerous if combined with the VLCD (very low calorie menu.) These over the counter drops have been addressed and dismissed largely by the FDA for good reason- they are NOT safe with the original protocol. Read: How to know if my Hcg Drops are Real, for more information.

Exercise is an important part of healthy living, even without wanting to lose weight. While the original protocol insistedexercise was not necessary, most of the evolvedprotocols include and encourage a consistent exercise routine simply because it is a healthy lifestyle option and the benefits are many.

For those currently active, in most cases, it is ok to continue as usual, although some may find it useful to lighten their workout for the first week of being on the protocol. For those who are new to working out, a low impact daily workout of at least 20 minutes a day is recommended to start. Recommended activities include: walking (brisk), swimming, yoga (no hot yoga practices yet), and pilates. Level of activity is extremely light the first week of phase 2 on the HCG Diet and increases as the individual feels ready. It is highly advised that the chosen workouts are something enjoyable, and not something you dread doing every day.

The HCG Diet can be complicated and so specific,you are almost certain to experiencechallenges along the way. While it might not seem welcome, It is a large part of the learning process for long term managementsuccess. There will be temptation to stray from your planned diet, and there may even be times when you stray by accident. Yes, it can happen: Aunt Sally forgot to tell you she adds sugar toher famous holiday vinaigrettedressing, or the restaurant neglected to tell you there's bread crumb mixed right into the hamburger patty which you so carefully ordered without a bun. Of course there's the good old oops, I assumed that hot sauce was just hot sauce and didn't realize it had 9 grams of sugar. There's your first lesson; always double-check ingredientsand don't eat it unless you're sure. A better lesson if you can't see all ingredients, don't eat it! The less processed foods you eat, the better. See, it's all part of the big picture and building the skillsto be fit and thin for life.

Original Hcg Diet Manuscript (available to download PDF and Audiobook)

Hcg Diet Food Lists

Hcg Diet Phase 2 Food List with Calorie Counts

Hcg Diet Coaching (FREE)

HCG Diet Myths & Misunderstandings

HCG Diet Tracker Spreadsheet

HCG Diet Acronyms and Abbreviations

Dr. Simeons manuscript, Pounds and Inches is the original book that outlines every detail of the Hcg weight loss protocol. Dr. Simeons has been very specific in his words, outlining all aspects of the diet. This includes the original 500 calorie menu, the effects of hCG on the body, hCG dosage, how to inject hCG, how to handle stalls and plateaus, and a solid overview transitioning from phase 2 to phase 3, which many dieters can find challenging.

Regardless of which version of the protocol the patient is following, it can still be helpful to read the manuscript. Not only does this allow the Hcg dieter to understand the intention of the diet, but it can also help establish a solid foundation of preparation, and help avoid challenges by answering many of the most frequently asked questions.

Our editor has taken the time to separate the original manuscript by chapter, making it easy to navigate and locate individual topics. We also offer an MP3 audio version (audiobook) of the Pounds and Inches manuscript, as well as a PDF version. These will be automatically sent to those who sign up for our newsletter but they can also be found here:

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Hcg Diet Plan: Get to Know the REAL Hcg Diet | HCG DIET ...

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Planet Biotechnology, Inc.

Posted: July 5, 2018 at 11:46 pm

Biodefense Since 2002 Planet Biotechnology has placed special emphasis on developing antibody therapeutics for emerging diseases, and biodefense countermeasures to known bio-warfare agents, including toxins, viruses and bacteria. With support from the National Institute of Allergy and Infectious Diseases (NIAID), we have developed an immunoadhesin for treatment and prevention of anthrax (PBI-220) and have tested it successfully in anthrax-infected monkeys as a therapeutic with an 80% survival rate when treatment is started after disease symptoms appear. We have conducted a pre-IND meeting with FDA and are seeking funding for the clinical testing of PBI-220.

Emerging Diseases Middle East Respiratory Syndrome (MERS) is a recently emerged disease caused by the MERS coronavirus (MERS-CoV) endemic to the Arabian Peninsula. It has already appeared in seven Middle Eastern countries and has traveled to European countries and South Korea as well. MERS-CoV causes a pneumonia-like disease that has a fatality rate approaching 40%. We have created and produced in green plants an immunoadhesin (DPP4-Fc) that has improved binding to MERS-CoV and have shown that it prevents the virus from infecting human lung cells in culture. In June 2015 we were awarded a Phase II SBIR grant from NIAID to support development of this candidate immunoadhesin. We will be testing DPP4-Fc in animal models of MERS-CoV based on our encouraging in vitro data.

Protein expression and purification services We have considerable experience expressing human and chimeric IgA and IgG antibodies, immunoadhesins, nanobodies, and other proteins. Planet Biotechnology is a DARPA contractor (contract no. HR001113D-0004) for production of recombinant proteins in Nicotiana benthamiana.

Our proprietary codon optimization method enables unprecedentedly high expression levels of recombinant proteins in both transiently and stably transformed tobacco plants. We have achieved expression of IgG1 at levels up to 3 grams/kg of plant biomass after vacuum-assisted agroinfiltration. These high levels of expression, along with our proprietary purification methods, result in high product yield and purity. We have research quantities of the following proteins available:

To find out more about our proteins and services, please email us atinfo@planetbiotechnology.com

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Planet Biotechnology, Inc.

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Cloning/Embryonic Stem Cells – National Human Genome …

Posted: July 5, 2018 at 11:43 pm

Cloning/Embryonic Stem Cells

The term cloning is used by scientists to describe many different processes that involve making duplicates of biological material. In most cases, isolated genes or cells are duplicated for scientific study, and no new animal results. The experiment that led to the cloning of Dolly the sheep in 1997 was different: It used a cloning technique called somatic cell nuclear transfer and resulted in an animal that was a genetic twin -- although delayed in time -- of an adult sheep. This technique can also be used to produce an embryo from which cells called embryonic stem (ES) cells could be extracted to use in research into potential therapies for a wide variety of diseases.

Thus, in the past five years, much of the scientific and ethical debate about somatic cell nuclear transfer has focused on its two potential applications: 1) for reproductive purposes, i.e., to produce a child, or 2) for producing a source of ES cells for research.

The technique of transferring a nucleus from a somatic cell into an egg that produced Dolly was an extension of experiments that had been ongoing for over 40 years. In the simplest terms, the technique used to produce Dolly the sheep - somatic cell nuclear transplantation cloning - involves removing the nucleus of an egg and replacing it with the diploid nucleus of a somatic cell. Unlike sexual reproduction, during which a new organism is formed when the genetic material of the egg and sperm fuse, in nuclear transplantation cloning there is a single genetic "parent." This technique also differs from previous cloning techniques because it does not involve an existing embryo. Dolly is different because she is not genetically unique; when born she was genetically identical to an existing six-year-old ewe. Although the birth of Dolly was lauded as a success, in fact, the procedure has not been perfected and it is not yet clear whether Dolly will remain healthy or whether she is already experiencing subtle problems that might lead to serious diseases. Thus, the prospect of applying this technique in humans is troubling for scientific and safety reasons in addition to a variety of ethical reasons related to our ideas about the natural ordering of family and successive generations.

Several important concerns remain about the science and safety of nuclear transfer cloning using adult cells as the source of nuclei. To date, five mammalian species -- sheep, cattle, pigs, goats, and mice -- have been used extensively in reproductive cloning studies. Data from these experiments illustrate the problems involved. Typically, very few cloning attempts are successful. Many cloned animals die in utero, even at late stages or soon after birth, and those that survive frequently exhibit severe birth defects. In addition, female animals carrying cloned fetuses may face serious risks, including death from cloning-related complications.

An additional concern focuses on whether cellular aging will affect the ability of somatic cell nuclei to program normal development. As somatic cells divide they progressively age, and there is normally a defined number of cell divisions that can occur before senescence. Thus, the health effects for the resulting liveborn, having been created with an "aged" nucleus, are unknown. Recently it was reported that Dolly has arthritis, although it is not yet clear whether the five-and-a-half-year-old sheep is suffering from the condition as a result of the cloning process. And, scientists in Tokyo have shown that cloned mice die significantly earlier than those that are naturally conceived, raising an additional concern that the mutations that accumulate in somatic cells might affect nuclear transfer efficiency and lead to cancer and other diseases in offspring. Researchers working with clones of a Holstein cow say genetic programming errors may explain why so many cloned animals die, either as fetuses or newborns.

The announcement of Dolly sparked widespread speculation about a human child being created using somatic cell nuclear transfer. Much of the perceived fear that greeted this announcement centered on the misperception that a child or many children could be produced who would be identical to an already existing person. This fear is based on the idea of "genetic determinism" -- that genes alone determine all aspects of an individual -- and reflects the belief that a person's genes bear a simple relationship to the physical and psychological traits that compose that individual. Although genes play an essential role in the formation of physical and behavioral characteristics, each individual is, in fact, the result of a complex interaction between his or her genes and the environment within which he or she develops. Nonetheless, many of the concerns about cloning have focused on issues related to "playing God," interfering with the natural order of life, and somehow robbing a future individual of the right to a unique identity.

Several groups have concluded that reproductive cloning of human beings creates ethical and scientific risks that society should not tolerate. In 1997, the National Bioethics Advisory Commission recommended that it was morally unacceptable to attempt to create a child using somatic cell nuclear transfer cloning and suggested that a moratorium be imposed until safety of this technique could be assessed. The commission also cautioned against preempting the use of cloning technology for purposes unrelated to producing a liveborn child.

Similarly, in 2001 the National Academy of Sciences issued a report stating that the United States should ban human reproductive cloning aimed at creating a child because experience with reproductive cloning in animals suggests that the process would be dangerous for the woman, the fetus, and the newborn, and would likely fail. The report recommended that the proposed ban on human cloning should be reviewed within five years, but that it should be reconsidered "only if a new scientific review indicates that the procedures are likely to be safe and effective, and if a broad national dialogue on societal, religious and ethical issues suggests that reconsideration is warranted." The panel concluded that the scientific and medical considerations that justify a ban on human reproductive cloning at this time do not apply to nuclear transplantation to produce stem cells. Several other scientific and medical groups also have stated their opposition to the use of cloning for the purpose of producing a child.

The cloning debate was reopened with a new twist late in 1998, when two scientific reports were published regarding the successful isolation of human stem cells. Stem cells are unique and essential cells found in animals that are capable of continually reproducing themselves and renewing tissue throughout an individual organism's life. ES cells are the most versatile of all stem cells because they are less differentiated, or committed, to a particular function than adult stem cells. These cells have offered hope of new cures to debilitating and even fatal illness. Recent studies in mice and other animals have shown that ES cells can reduce symptoms of Parkinson's disease in mouse models, and work in other animal models and disease areas seems promising.

In the 1998 reports, ES cells were derived from in vitro embryos six to seven days old destined to be discarded by couples undergoing infertility treatments, and embryonic germ (EG) cells were obtained from cadaveric fetal tissue following elective abortion. A third report, appearing in the New York Times, claimed that a Massachusetts biotechnology company had fused a human cell with an enucleated cow egg, creating a hybrid clone that failed to progress beyond an early stage of development. This announcement served as a reminder that ES cells also could be derived from embryos created through somatic cell nuclear transfer, or cloning. In fact, several scientists believed that deriving ES cells in this manner is the most promising approach to developing treatments because the condition of in vitro fertilization (IVF) embryos stored over time is questionable and this type of cloning could overcome graft-host responses if resulting therapies were developed from the recipient's own DNA.

For those who believe that the embryo has the moral status of a person from the moment of conception, research or any other activity that would destroy it is wrong. For those who believe the human embryo deserves some measure of respect, but disagree that the respect due should equal that given to a fully formed human, it could be considered immoral not to use embryos that would otherwise be destroyed to develop potential cures for disease affecting millions of people. An additional concern related to public policy is whether federal funds should be used for research that some Americans find unethical.

Since 1996, Congress has prohibited researchers from using federal funds for human embryo research. In 1999, DHHS announced that it intended to fund research on human ES cells derived from embryos remaining after infertility treatments. This decision was based on an interpretation "that human embryonic stem cells are not a human embryo within the statutory definition" because "the cells do not have the capacity to develop into a human being even if transferred to the uterus, thus their destruction in the course of research would not constitute the destruction of an embryo." DHHS did not intend to fund research using stem cells derived from embryos created through cloning, although such efforts would be legal in the private sector.

In July 2001, the House of Representatives voted 265 to 162 to make any human cloning a criminal offense, including cloning to create an embryo for derivation of stem cells rather than to produce a child. In August 2002, President Bush, contending with a DHHS decision made during the Clinton administration, stated in a prime-time television address that federal support would be provided for research using a limited number of stem cell colonies already in existence (derived from leftover IVF embryos). Current bills before Congress would ban all forms of cloning outright, prohibit cloning for reproductive purposes, and impose a moratorium on cloning to derive stem cells for research, or prohibit cloning for reproductive purposes while allowing cloning for therapeutic purposes to go forward. As of late June, the Senate has taken no action. President Bush's Bioethics Council is expected to recommend the prohibition of reproductive cloning and a moratorium on therapeutic cloning later this summer.

Prepared by Kathi E. Hanna, M.S., Ph.D., Science and Health Policy Consultant

Last Reviewed: April 2006

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Stem Cell Therapy in Mexico – Official Treatment Center

Posted: July 4, 2018 at 2:43 pm

Adipose Derived Stem Cells:

Adipose derived stem cells are obtained from a sample of human abdominal fat tissue. The cells themselves are not fat. They are encased in and surrounded by fat tissue.Once the fat sample has been obtained, a special enzyme is used to melt-away the fat, and leave only something called SVF (short for Stromal Vascular Fraction), which contains stem cells, accessory cells and growth factors.

This SVF is washed and purified in the lab, and is then isolated for infusion or injection.

This method provides a much larger number of stem cells than bone marrow or peripheral blood, making it more efficient and highly effective in a variety of conditions, especially those that require creation of new blood vessels, and repairing tissues damaged due to lack of oxygenation.

Whartons Jelly Derived Stem cells:

Unlike Fat, Bone marrow or other tissues that can be harvested for stem cell isolation, Whartons Jelly does not contain SVF. It is a gelatinous substance found in the umbilical chord, which separates maternal and fetal tissues, acting as a kind of buffer so that these tissues dont come into direct contact with each other. This particular characteristic is the reason they became known as universal donor cells, since they are able to interact with any tissue, in any host, without causing any form of immune response.

Unlike cells obtained with SVF, stem cells in Whartons jelly are not found grouped with other cells types or blood products. They can be obtained in much larger numbers, and are already completely isolated, which means that you get stem cells exclusively.

The fact that they are obtained from umbilical chords, donated by pre-screened donors after their pregnancy has come to full-term, means that they are much easier to harvest, in much larger numbers, and without the need for a specific, invasive and painful procedure. It also means that because of the incredibly large number of cells that can be obtained, they can be cryo-preserved and stored at pre-determined dose sizes in individual containers for specific uses.

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Stem Cell Therapy in Mexico - Official Treatment Center

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Gene Therapy Retrovirus Vectors Explained

Posted: July 4, 2018 at 2:41 pm

A retrovirus is any virus belonging to the viral family Retroviridae. All The genetic material in retroviruses is in the form of RNA molecules, while the genetic material of their hosts is in the form of DNA. When a retrovirus infects a host cell, it will introduce its RNA together with some enzymes into the cell. This RNA molecule from the retrovirus must produce a DNA copy from its RNA molecule before it can be considered part of the genetic material of the host cell. Retrovirus genomes commonly contain these three open reading frames that encode for proteins that can be found in the mature virus. Group-specific antigen (gag) codes for core and structural proteins of the virus, polymerase (pol) codes for reverse transcriptase, protease and integrase, and envelope (env) codes for the retroviral coat proteins (see figure 1). Figure 1. Genome organisation of retroviruses.

The process of producing a DNA copy from an RNA molecule is termed reverse transcription. It is carried out by one of the enzymes carried in the virus, called reverse transcriptase. After this DNA copy is produced and is free in the nucleus of the host cell, it must be incorporated into the genome of the host cell. That is, it must be inserted into the large DNA molecules in the cell (the chromosomes). This process is done by another enzyme carried in the virus called integrase (see figure 2).

Now that the genetic material of the virus is incorporated and has become part of the genetic material of the host cell, we can say that the host cell is now modified to contain a new gene. If this host cell divides later, its descendants will all contain the new genes. Sometimes the genes of the retrovirus do not express their information immediately.

Retroviral vectors are created by removal op the retroviral gag, pol, and env genes. These are replaced by the therapeutic gene. In order to produce vector particles a packaging cell is essential. Packaging cell lines provide all the viral proteins required for capsid production and the virion maturation of the vector. These packaging cell lines have been made so that they contain the gag, pol and env genes. Early packaging cell lines contained replication competent retroviral genomes and a single recombination event between this genome and the retroviral DNA vector could result in the production of a wild type virus. Following insertion of the desired gene into in the retroviral DNA vector, and maintainance of the proper packaging cell line, it is now a simple matter to prepare retroviral vectors (see figure 3).

One of the problems of gene therapy using retroviruses is that the integrase enzyme can insert the genetic material of the virus in any arbitrary position in the genome of the host. If genetic material happens to be inserted in the middle of one of the original genes of the host cell, this gene will be disrupted (insertional mutagenesis). If the gene happens to be one regulating cell division, uncontrolled cell division (i.e., cancer) can occur. This problem has recently begun to be addressed by utilizing zinc finger nucleases or by including certain sequences such as the beta-globin locus control region to direct the site of integration to specific chromosomal sites.

Gene therapy trials to treat severe combined immunodeficiency (SCID) were halted or restricted in the USA when leukemia was reported in three of eleven patients treated in the French X-linked SCID (X-SCID) gene therapy trial. Ten X-SCID patients treated in England have not presented leukemia to date and have had similar success in immune reconstitution. Gene therapy trials to treat SCID due to deficiency of the Adenosine Deaminase (ADA) enzyme continue with relative success in the USA, Italy and Japan.

As a reaction to the adverse events in the French X-SCID gene therapy trial, the Recombinant DNA Advisory Committee (RAC) sent a letter to Principal Investigators Conveying RAC Recommendations in 2003. In addition, the RAC published conclusions and recommendations of the RAC Gene Transfer Safety Symposium in 2005. A joint working party of the Gene Therapy Advisory Committee and the Committee on Safety of Medicines (CSM) in the UK lead to the publication of an updated recommendations of the GTAC/CSM working party on retroviruses in 2005.

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Gene Therapy Retrovirus Vectors Explained

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Nervous System Stem Cells Can Replace Themselves, Give …

Posted: July 3, 2018 at 8:44 am

A green fluorescent protein-labeled neural stem cell clone contains the mother stem cell with neuronal and astroglial progeny within the mouse brain.

Image by Michael A. Bonaguidi, Johns Hopkins Medicine.

A Johns Hopkins team has discovered in young adult mice that a lone brain stem cell is capable not only of replacing itself and giving rise to specialized neurons and glia important types of brain cells but also of taking a wholly unexpected path: generating two new brain stem cells.

A report on their study appears June 24 in Cell.

Although it was known that the brain has the capacity to generate both neurons, which send and receive signals, and the glial cells that surround them, it was unclear whether these various cell types came from a single source. In addition to demonstrating that a single radial glia-like (RGL) brain cell is able to generate two very different functional cell types, the Hopkins researchers, by following the fates of single cells over time, found that a single brain stem cell can even produce two stem cells like itself.

Now we know they dont just maintain their numbers, or go down in number, but that stem cells can amplify, says Hongjun Song, Ph.D., professor of neurology and neuroscience and director of the Stem Cell Program in the Institute for Cell Engineering, the Johns Hopkins University School of Medicine. If we can somehow cash in on this newly discovered property of stem cells in the brain, and find ways to intervene so they divide more, then we might actually increase their numbers instead of losing them over time, which is what normally happens, perhaps due to aging or diseases.

The researchers findings hinged on a decision to single out and follow lone, radial glia-like cells, instead of labeling and monitoring entire stem cell populations in the mouse brain. They took this approach because they suspected radial glia-like cells were essentially stem cells, having been shown in previous studies to give rise to neurons.

Using mice genetically modified with special genes that color-code cells for easy labeling and tracking, the Hopkins team injected a very small amount of a chemical into about 50 mouse brains to induce extremely limited cell labeling.

Its a simple idea that forced us to confront a lot of complex technical issues, Song says. With so many millions of cells in the relatively large mouse brain, labeling a single stem cell and then chasing its family history was like finding a needle in a haystack.

The scientists developed computer programs and devised a new imaging technique that allowed them to examine stained slices of the mouse brain and, ultimately, follow single, randomly chosen radial glia-like stem cells over time. The method allowed them to track down all the new cells derived from a single original stem cell.

We reconstituted single stem cells family trees to look at the progeny they gave rise to, says Guo-li Ming, associate professor of neurology and neuroscience and a member of the Neuroregeneration Program in the Institute for Cell Engineering. We discovered that single cells in an intact animal nervous system absolutely do exhibit stem-cell properties; they are capable of both replicating themselves and producing different types of differentiated neural progeny.

The team followed the fates of all the marked radial glia-like stem cells for at least a month or two, and examined some a full year later to discover that even over the long term, the mother cell was still generating itself as well as different kinds of progeny.

In addition, the researchers investigated how these RGLs were activated on a molecular level, focusing, in particular, on the regulatory role of an autism-associated gene called PTEN. Conventional wisdom was that deleting this gene led to an increase in stem-cell activation. However, the scientists demonstrated that was a transient effect in the mouse brains, and that, ultimately, PTEN deletion leads to stem-cell depletion.

Support for this research came from the National Institutes of Health, the Brain and Behavior Research Foundation, and the Maryland Stem Cell Research Foundation.

Authors of the paper, in addition to Hongjun Song and Guo-li Ming, are Michael A. Bonaguidi, Michael A. Wheeler, Jason S. Shapiro and Gerald. J. Sun, all of Johns Hopkins.

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Nervous System Stem Cells Can Replace Themselves, Give ...

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