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Floored by diabetes diagnosis, Richard Fink fought back – Buffalo News

Posted: May 20, 2017 at 6:42 am

Richard Fink was an All-High first baseman who weighed about 165 pounds when he played for the Bennett High School baseball team during the mid-1970s. He spent the 1980s and 90s playing Muni and suburban hardball with teams based near his Getzville home, as well as pickup basketball up to three times a week.

Im very competitive, he said.

Fink, 62, has worked the last three decades as a legal process server. Over the years, he spent a growing amount of time behind a desk or the wheel of a car. He ate lots of food on the go and enjoyed his share of the standard Buffalo diet: breakfast sandwiches, chicken wings and pizza.

The nearly 6-foot-tall Fink weighed 215 pounds when a case of pneumonia knocked him off his feet for two months early last year. He packed on another 15 pounds as he recovered.

Meanwhile, his blood sugar level crept into the diabetes range.

Ive played sports my whole life, so when youre diagnosed with diabetes its a shock, he said.

[BELOW: Diabetes ABCs, key prediabetes and diabetes test levels]

Fink was not at all comfortable joining the legion of 115 million Americans roughly one in three with diabetes or prediabetes. His primary care doctor put him on the oral medication Metformin and helped him understand the dangers unaddressed high blood sugar levels can bring: poor circulation that boosts the risk of blindness and kidney failure, heart attack, stroke and limb amputation.

Fink was floored.

If scientists tell you that you can go blind looking at the sun during an eclipse, you dont look. Its an immediate reaction, he said. But if it comes down to eating or other choices, its OK, because nothing happens immediately to you. But then, 20 years later, that accumulation of poor eating habits and behavior, it catches up with you. The bill comes due.

Fink decided to beat back diabetes. Heres how.

Start with a plan

Richard Fink, left, pledged to amp up his weekly workout regimen and change his diet after he was diagnosed with diabetes. (Robert Kirkham/Buffalo News)

Fink started his Type 2 diabetic journey last spring after lab tests showed his fasting blood sugar level at 338 milligrams per deciliter, more than three times the normal level. His A1C reading an average blood sugar percentage over two to three months stood at 7.2, also in the diabetes range.

The best thing someone can do when they get diagnosed with diabetes or prediabetes is to lose weight, he said. Its the essential key to ridding yourself of diabetes.

[RELATED: Type 1 diabetic from WNY touts little-used inhalable insulin]

Fink had long been interested in the training regimens of top athletes, even though he didnt always follow them himself, so he started from a place he understood: He needed to burn off more calories than he consumed.

Research shows that losing 15 percent of your body weight can lower the risk of Type 2 diabetes by more than 50 percent, according to the American Diabetes Association.

That kind of weight loss needed to start with a new mental approach, Fink reasoned. He started to make better, more educated health and fitness choices going forward every day and not get too discouraged if he slipped up occasionally.

The problem most people have is they want instant gratification, he said. You dont get out of shape overnight. You dont get in shape overnight. Its a process.

Nutrition is key

A Mediterranean diet including plenty of chicken and fish has been part of Richard Fink's nutrition plan. (Robert Kirkham/Buffalo News)

Diet and exercise has to be part of any diabetic regime, no matter what, said Dr. Howard Lippes, a Williamsville endocrinologist.

Fink was determined to add more exercise to his weekly regimen. For him, the remnants of his past made this the easier part. The more important thing to remember is that you cannot out-train a bad diet, he said.

He pretty much knew how to eat right, but the new numbers in his life underlined that he didnt make the best choices often enough. He turned decidedly to a Mediterranean diet. He gave up cereal, white breads and other processed foods made with simple carbohydrates and added sugars. Simple carbs as opposed to their more complex kin, found mostly in vegetables raise blood sugar levels, which promote body inflammation that fuels many chronic diseases, including diabetes, the seventh leading cause of death in the U.S.

Fink fed his better habits with information gleaned from books, magazines and (shameful plug intended) WNY Refresh. He tends to eat eggs for breakfast, a light, chicken-based meal for lunch and dinners rich in chicken, fish, fruits and vegetables. He drinks plenty of water. He has never smoked anything and doesnt drink alcohol, which in excess also promotes disease.

Do I still love wings? Absolutely, he said. Do I still have them once in a while? Sure, but I have changed to a lot of healthy eating. You need to have chicken and fish and salads, and be careful on dressings and toppings. Youve got to read labels, too. I never thought I needed to. The more informed you can become, the better.

Exercise counts

Each member who takes group classes at Orangetheory wears a heart rate monitor to let them know when they approach their maximum heart rate. (Robert Kirkham/Buffalo News)

There are plenty of days I dont want to work out, Fink said, but those are the days that are most important. You have to force yourself to do what you need to do to burn calories, and drive by places where you shouldnt be eating.

[RELATED STORY: Falls Memorial studies new wound treatment]

Fink turned to Orangetheory in Amherst to burn calories. He takes a late afternoon class five to six days a week in which workouts vary, keeping things interesting and targeting different muscle groups. Trainers help guide his fitness and exercise form during hourlong workouts split between challenging treadmill work and circuit training that includes rowing machines, free weights and TRX resistance bands. The workouts focus on splat points one point for every minute in the Orange zone, 84 to 90 percent of your maximum heart rate. You want you to get 12 to 20 points per session to develop an afterburn that keeps you shedding calories for a day or so after your workout.

When I go for my workout, I burn 800, 900 calories, Fink said, so technically, after my workout, I havent eaten because Ive burned off breakfast and lunch.

Your heart is a muscle that Fink compares to a Lamborghini. If you walk every day, thats good, but its like driving that car at 25 miles an hour, he said. Youve got to open that up, raise that heart rate every once in a while, to make sure youre working it.

Its important to check with your doctor before starting an exercise routine.

A new way of life

Fink has learned since last spring that hard, consistent work can pay off. Being healthy is a second job, he said.

He has lost 30 pounds since last year and his A1C level is down to 5.4 below the prediabetic and diabetic range. He is taking a quarter the dose of his original diabetes prescription and looks to soon get off it completely. He aims to stay this way.

You cant say, I got rid of it, so I can eat doughnuts again. You dont want to do that, he said. You can blow a whole workout with foods like that. You have to combine exercise with diet. People say they cant lose weight. Dont say you cant. Say youre not going to make the effort. If youre not making the effort, who are you cheating?

The ABCs of diabetes

Type 1 diabetes:Normally diagnosed in children and young adults. The body does not produce insulin, a hormone needed to convert sugar, starches and other food into energy the bodys cells need for daily life. Only 5 percent of people with diabetes have this form of the disease, which is treated with insulin therapy.

Type2diabetes:Almost 30 million Americans have Type 1 or 2 diabetes; Type 2 is by far the most common form. The body does not produce enough insulin, or the cells ignore the insulin, and glucose builds up in the bloodstream instead.

It is more common as a percentage among African-Americans, Latinos, Native Americans and Asian Americans. Roughly one in four Americans age 65 and older has diabetes.

More than 80 percent of those with Type 2 diabetes are overweight.

Complications, which can vary widely and by degree, include glaucoma, cataracts and other eye problems; numbness in the feet; skin infections and skin disorders; hearing loss; depression; and high blood pressure, which raises your risk for heart attack, stroke and kidney disease.

High blood sugar can overwork the kidneys, causing them to stop working properly. When diagnosed early, kidney disease can be slowed with treatment; when diagnosed later, kidney failure usually results.

Prediabetes:An estimated 86 million Americans have prediabetes. Before people develop Type 2 diabetes, they almost always have prediabetes, or blood glucose levels that are higher than normal but not yet high enough to be diagnosed as diabetes.

Recent research has shown that some long-term damage to the body, especially the heart and circulatory system, may already be occurring during prediabetes.

Key tests

A1C: Measures the average blood sugar (glucose) level percentage over 2-3 months.

Results of A1C:

Normal:less than 5.7 percent

Prediabetes:5.7 to 6.4 percent

Diabetes:6.5 percent or higher

Fasting Plasma Glucose: Levels of milligrams per deciliter of blood glucose after fasting at least 8 hours.

Results of FPG:

Normal:less than 100 mg/dl

Prediabetes:100-125 mg/dl

Diabetes:126 mg/dl or higher

Oral Glucose Tolerance Test: Levels of milligrams per deciliter before and 2 hours after a sweet drink; most often used to test for diabetes during pregnancy.

Results of OCTT:

Normal:less than 140 mg/dl

Prediabetes:140-129 mg/dl

Diabetes:200 mg/dl or higher

Prevention and treatment

Exercise and a healthy diet are key to preventing prediabetes and diabetes, as well as better managing them. (Robert Kirkham/Buffalo News)

Regular exercise and a healthy diet void of processed foods can help.

Smoking, high blood pressure, abnormal blood cholesterol levels, being overweight and being sedentary can worsen diabetes.

Medications and dialysis are treatments that may be required.

Sources: American Diabetes Association (diabetes.org), Seneca Nation of Indians Diabetes Foundation

Resourcesfrom the Buffalo & Erie County Public Libraries

What to Expect When You Have Diabetes: 170 tips for living well with diabetes, American Diabetes Association

Whole Cooking & Nutrition: An everyday superfoods approach to planning, cooking, and eating with diabetes, Katie Cavuto

Your Type 2 Diabetes Action Plan: Tips, techniques, and practical advice for living well with diabetes, American Diabetes Association

The Diabetes Reset: Avoid it, control it, even reverse it a doctors scientific program, George King

Diabetes Without Drugs: The 5-step program to control blood sugar naturally and prevent diabetes complications, Suzy Cohen

For more information, visit buffalolib.org.

email: refresh@buffnews.com

Twitter: @BNrefresh, @ScottBScanlon

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MRMC’s diabetes education program receives certification – Magnoliareporter

Posted: May 20, 2017 at 6:42 am

The American Diabetes Association Education Recognition Certificate for a quality diabetes self-management education program was recently awarded to the Magnolia Regional Medical Center program.

ADSA believes that this program offers high-quality education that is an essential component of effective diabetes treatment.

The Associations Education Recognition Certificate assures that educational programs meet the National Standards for Diabetes Self-Management Education Programs. These Standards were developed and tested under the auspices of the National Diabetes Advisory Board in 1983 and were revised by the diabetes community in 1994, 2000, 2007 and 2012.

Programs apply for recognition voluntarily. Programs that achieve recognition status have a staff of knowledgeable health professionals who can provide participants with comprehensive information about diabetes management.

The process gives professionals a national standard by which to measure the quality of services they provide, said Rex Jones, CEO. And, of course, it assures the consumer that he or she will likely receive high-quality service.

Education Recognition status is verified by an official certificate from ADA and is awarded for four years.

According to the American Diabetes Association, there are 29.1 million people or 9.3% of the population in the United States who have diabetes. While an estimated 21 million have been diagnosed, unfortunately, 8.1 million people are not aware that they have this disease. Each day more than 3,900 people are diagnosed with diabetes.

Many will first learn that they have diabetes when they are treated for one of its life-threatening complications heart disease and stroke, kidney disease, blindness, and nerve disease and amputation.

About 1.4 million new cases of diabetes were diagnosed in people aged 20 years or older in 2014 in the U.S. Diabetes contributed to 234,051 deaths in 2010, making it the seventh leading cause of death in the U.S. Overall, the risk for death among people with diabetes is 50% greater than that of people of similar age but without diabetes.

The American Diabetes Association is the nations leading non-profit health organization supporting diabetes research, advocacy and information for health professionals, patients and the public. Founded in 1940, the association conducts programs in communities nationwide.

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Designing better drugs to treat type 2 diabetes – Science Daily

Posted: May 20, 2017 at 6:42 am


NDTV
Designing better drugs to treat type 2 diabetes
Science Daily
"Type two diabetes is characterised by resistance to insulin with subsequent high blood sugar which leads to serious disease. It is usually associated with poor lifestyle factors such as diet and lack of exercise," says lead researcher Dr John Bruning ...
Soon, Anti-Diabetes Drugs to Replace Painful Insulin JabsNDTV
New diabetes drug may soon replace insulin jabsDeccan Chronicle
Suffering from diabetes? Here's some good news for youEconomic Times
Diabetes.co.uk -Zee News
all 12 news articles »

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Buffalo teacher a key advocate in breakthrough diabetes drug – Buffalo News

Posted: May 20, 2017 at 6:42 am

Eric Fenar is a Type 1 diabetic whoexercises regularly,rigorously measures his blood sugar levels and takes othersteps to stayhealthy.

Still, he says,"In life, it's tough to eat tofu and salad and grilled chicken every day."

He likes Asian food, pizza and beer too much for that.

This is why Fenar has become one of the biggest volunteer pitchmen in the country for an inhalable insulin drug called Afrezza a drug that has helped the 34-year-old Buffalo public school teacher reach the most stable blood glucose levels he's had since he was diagnosed at age 10.

"There is a little bit of a learning curve," he said, "but now I have the power to fight those high blood sugars. Life is much more flexible."

Fenar discovered Afrezza during one of his routine online searches about better diabetes management. He testified at a 2014 federal Food and Drug Administration hearing to tout the promise of Afrezza for those like himself before he'd even tried the drug. He met Al Mann, the creator, before the billionaire businessman died in February 2016.

[RELATED STORY: Floored by diabetes diagnosis, Richard Fink fought back]

Fenar grew up in Lancaster. He remembers the weekend he was diagnosed with Type 1 diabetes, because he watched on a hospital television set on Oct. 23, 1993, as Joe Carter hit a home run to help the Toronto Blue Jays win the World Series.

His diagnosis meant that his body produced no insulin to help him break down food. It also meant a lifetime of insulin injections and great sacrifices as he kept vigilant watch on his blood sugar levels or so it seemed at the time.

"I want people to be aware of this option," Eric Fenar says. "This is what I needed: a faster insulin."

"My family has always been up to date on the latest technologies," he said. "I was up to date on the shots for two years and mom read about the insulin pump. Back then it was only for people 18 and over. At this point, I was about 12."

His mother, Susan, a nurse, convinced doctors and health insurers that Fenar was capable of using a pump to monitor his glucose levels and give himself insulin without dispensing a potentially fatal overdose.

Years later, he started to use a Dexcom, a Bluetooth-compatible device that reads blood sugar without the need to do a finger-prick blood test. This allowed him to use his pump, or an injection, to push the correct amount of insulin into his bloodstream.

The challenge, Fenar said, is the lag time that comes with injectable insulin. It can take 45 minutes to an hour for the effect to take hold causing damage from high blood sugar and running the risk of an insulin crash or overflow, particularly an hour or so before or after meals.

"The thing that got me is Afrezza starts to work in 12 to 15 minutes," he said.

Dr. Howard Lippes, a Williamsville endocrinologist, assistant clinical professor with the University at Buffalo medical school and owner of R&B Medical Group, prescribes the inhalable drug to about 15 to 20 percent of his Type 1 adult diabetic patients.

Some use it regularly, others intermittently "to correct high glucose," he said. "There are pros and cons. It's not better. It's different."

Lippes doesn't count Afrezza's inhalability as a big advantage, "given the ease of today's insulin pen devices, which are simple and painless," he said. But he and patients who use it appreciate the speed in which it can control blood sugar levels.

For example, those like Fenar who enjoy pizza. When someone without diabetes eats pizza, or other food or drink loaded with carbs, the pancreas starts making insulin right away to compensate for the spike in blood sugar, which can cause inflammation and damage the cardiovascular system.

"If you're diabetic and eat a meal, you've got to give the insulin a running start if you want to catch the blood sugar before it goes up," Lippes said. "The timing of the meal can be a bit daunting. The Afrezza works really quickly, so that is an advantage for some patients."

It also has a shorter "tail" than injectable insulins, leaving the bloodstream faster so the danger of a lingering low blood sugar reaction diminishes, the doctor said.

[RELATED STORY: Falls Memorial studies new wound treatment]

Lippes prescribes Afrezza almost exclusively for those with Type 1 diabetes. It costs about the same as injectable insulin and is covered by most health insurance plans, he said.

Those with a cough or cold may not want to use it, Lippes said, and it is not recommended for those with asthma or chronic lung diseases like COPD. Its use is discouraged by those who smoke, "not that any diabetic should do that," Lippes said.

A small percentage of patients who try Afrezza get a throat irritation or cough, though the majority of patients tolerate it well.

Despite its advantages, the inhalable drug has yet to reach a critical mass in the diabetes drug industry.

Creator Mann ran 17 companies that helped improve pacemakers, cochlear implantsand insulin pumps. He put $1 billion of his own money into Afrezza before he died, at age 90, and also was at work on developing an artificial retina.

Lippes blamed lagging Afrezza sales on the relatively small size of the drug-making company, MannKind, as well as the first inhalable drug, Exubera, which Pfizer introduced more than a decade ago, then pulled from the market in 2007. In that case, the inhalation device was so big, it couldn't fit into a purse and looked like something you'd use to smoke illegal drugs. It proved too cumbersome for most who tried it, Lippes said.

He described the Afrezza inhaler as "a little whistle device," which dispenses powdered insulin in 4-, 8- and 12-unit doses.

The Afrezza inhaler is a whistlelike device, which dispenses powdered insulin in 4-, 8- and 12-unit doses.

Fenar needs a basal insulin injection once a day, to keep his sugar levels stable while he sleeps. Otherwise, he has found reliability in the drug and delivery method he advocates.

"With Afrezza, I was able to get off my insulin pump," he said. "I used to carry what I called a diabetic man purse," with extra pump supplies and extra insulin. "Now, when I leave the house, I have a tenth of what I used to carry.I'm not connected to anything. And for my insulin needs, I take Afrezza.

"I want people to be aware of this option. This is what I needed: a faster insulin."

For Lippes, the drug represents one of several exciting new changes in diabetes treatment.

The next, he said, is the sensor augmented insulin pump. Medtronics Corp. which recently hit the market with its variety, the 670G Pump refers to it as "the artificial pancreas."

"It's not quite there yet," Lippes said, "but we're on the threshold of big changes with these new glucose sensor devices that can tell the insulin pump what to do in real time. I have patients lined up to get it."

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Tim Cook is reportedly testing Apple Watch-connected diabetes tracker – TNW

Posted: May 20, 2017 at 6:42 am

All signs seem to suggest Apple is gearing up to release a special blood sugar trackerfor the Apple Watch.

As it turns out, none other than CEO Tim Cook has been spotted wearing what appeared to be an Apple Watch-connected glucose tracker in the vicinity of the company campus, CNBC reports.

Unfortunately, details remain pretty scarce as of now with the exception that the new wearable module is directly connected to the Watch.

Assuming the Big A manages to fine-tune the rumored blood sugar tracker, the technology could become a must-have for millions of people suffering from or at risk of getting diabetes.

Apple was first rumored to be working on a dedicated diabetes wearable back in April, when CNBC reported the company has assembled a secret super-team of bioengineers to craft a solution for tracking blood sugar levels with the Watch.

Speaking at the University of Glasgow earlier in February, Cook said he had been wearing a continuous glucose monitor for a few weeks but stopped short of making any significant revelation about the gadget.

One thing the Apple chief clarified though is that the device would also make it easier for people to responsibly monitor their blood sugar levels and avoid health complications.

Its mentally anguishing to stick yourself many times a day to check your blood sugar, he commented. There is lots of hope out there that if someone has constant knowledge of what theyre eating, they can instantly know what causes the response and that they can adjust well before they become diabetic.

on CNBC

Read next: Microsoft's new Surface Pro just leaked, but it's not a 'Pro 5'

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Is Autologous Heamatopoietic Stem Cell Transplantation Still Viable for MS? – LWW Journals

Posted: May 19, 2017 at 5:48 am

FitzGerald, Susan

doi: 10.1097/01.NT.0000520472.01901.8f

Features

Two new reports on autologous hematopoietic stem cell transplantation (AHSCT) for multiple sclerosis (MS) indicate that the therapy may benefit some MS patients. But whether AHSCT is viable is a matter of debate among some MS experts, who contend that the regimen could be toxic, leading to infection and death.

Two new reports on autologous hematopoietic stem cell transplantation (AHSCT) for multiple sclerosis (MS) indicate that the therapy may benefit some MS patients. But whether AHSCT is viable is a matter of debate among some MS experts, who contend that the regimen, which uses a combination of cytotoxic drugs to ablate the immune system in an attempt to reset the immunological memory could be toxic, leading to infection and death.

Experts who were not involved with the study said that newer, second generation MS drugs may be safer options, though few studies comparing the method with these drugs have been undertaken.

The first new report, published in the April 28 online edition of Neurology, provided a meta-analysis of 15 studies involving 764 MS patients who underwent AHSCT. The report found that the risk-benefit profile of the therapy makes it best suited for patients who have aggressive, relapsing-remitting MS who have not yet become highly disabled.

The second report, which provided long-term outcomes for 281 MS patients from an observational, retrospective study, found that almost half of the patients remained free from neurological progression five years after AHSCT. The study, published in the April edition of JAMA Neurology, reported that younger age, relapsing form of MS, fewer prior immunotherapies, and lower baseline EDSS [Expanded Disability Status Scale] score were factors associated with better outcomes.

Maria Pia Sormani, PhD, professor of biostatistics at the University of Genoa in Italy, and lead author of the report in Neurology, told Neurology Today that skepticism about the treatment approach is likely due to multiple factors.

MS is not a lethal disease, and this procedure is very invasive and has a non-negligible mortality risk, said Dr. Sormani, who also was a study author on the JAMA Neurology study. The lack of data from a rigorous clinical trial of AHSCT for MS has also been problematic.

To gain a clearer picture of what the current evidence shows, her team's meta-analysis pooled data from 15 studies, mostly open label, from January 1991 to July 2016. The researchers found that treatment-related mortality (TRM) declined during the period covered by the review, likely a result of improvements in transplant techniques, more clinical experience, and better patient selection, Dr. Sormani said. Overall TRM was 2.1 percent, but after 2005 it was 0.3 percent.

The meta-analysis found that the rate of disease progression in patients was 17.1 percent at two years following AHSCT and 23.3 percent at five years. The analysis also found that 83 percent of patients had no evidence of disease activity (NEDA) at two years, and 67 percent had no evidence at five years. Doing the transplant earlier, before the patient develops much disability seems advantageous, Dr. Sormani said.

The meta-analysis had the usual limitations of such reviews, she noted. The original studies were not all designed or executed in the same way, patient selection and study methodology were not uniform, and transplant techniques and protocols varied.

Even with advanced immunotherapy, such as natalizumab or alemtuzumab, only 32-39 percent maintained NEDA at two years in the phase II clinical trials, wrote Joachim Burman, MD, PhD, of Uppsala University in Sweden and Robert Fox, MD, of the Cleveland Clinic, in the editorial accompanying the paper. They agreed with the research team that the approach is more likely to benefit those with RRMS, not those with progressive forms of MS.

The report in JAMA Neurology included data on 281 patients from 25 centers who underwent AHSCT between January 1995 and December 2006. Seventy-eight percent of the patients had progressive forms of MS. The median follow-up was 6.6 years, with some patients followed for as long as 16 years

The five-year probability of progression-free survival was 46 percent and overall survival was 96 percent, the research team headed by Paolo A. Muraro, MD, a clinical reader in neuroimmunology and deputy head of the division of brain sciences at Imperial College London.

Factors associated with neurological progression after transplant were older age, progressive (versus relapsing) form of MS, more than two previous disease-modifying therapies, and higher baseline EDSS scores.

An accompanying editorial coauthored by Michael K. Racke, MD, professor of neurology and neuroscience at Ohio State University, noted that while the transplant therapy appears to favor those with RRMS with aggressive breakthrough disease, it Z

Dr. Racke told Neurology Today in an interview that he is currently planning a multicenter randomized controlled trial, which will include 55 RRMS patients in each arm. The study will compare AHSCT using what is considered a medium-intensity myelobation (BEAM) technique to best available drug treatment (whatever treatment a given patent is taking).

Dr. Racke said one question that needs to be further considered is, When is the best time to do a transplant? He said drug therapies need to be given a chance, but earlier might be better than later because once you start getting damage to the central nervous system we can't really fix that.

He said the upcoming trial will likely include cost analyses to compare the cost of long-term drug therapy to the mostly upfront costs of transplant, which is thought to be a once-and-done procedure.

Commenting on the two studies, Timothy L. Vollmer, MD, FAAN, professor of neurology at University of Colorado Health Sciences Center and co-director of the Rocky Mountain MS Clinic at Anschutz Medical Center, expressed skepticism about using AHSCT, particularly in light of effectiveness of the second-generation MS drugs that have come into use, such as natalizumab for JCV negative patients, fingolimod, dimethyl fumarate, and ocrelizumab.

Dr. Vollmer said most studies of AHSCT for MS were done before the newer drugs were available. He is concerned about both the immediate risks (infection, death) and potential long-term consequences of undergoing a toxic regimen to eradicate the immune system, noting that it could cause brain atrophy, already a concern for MS patients.

Mark S. Freedman, MD, professor of neurology at the University of Ottawa, senior scientist at The Ottawa Hospital Research Institute, and director of the Multiple Sclerosis Research Unit at The Ottawa Hospital-General Campus, is more sanguine about the procedure.

In a 2016 report in The Lancet, he and a colleague described outcomes for 24 RRMS patients who underwent transplant after failing drug therapy. Dr. Freedman said he has done about 25 more cases since the study came out. He said no patient has experienced a clinical relapse following transplant, none has evidence of new brain lesions on MRI, and none requires disease-modifying medication.

Dr. Freedman said there is a high level of interest in the procedure among MS patients, but it isn't for everyone. Patients must be carefully selected for the procedure, and undergo an aggressive chemotherapy regimen to eliminate their immune system, he said, noting that those with a high inflammatory component to their disease are ideal. Harvested stem cells undergo a special sorting technique at his center before being infused into the body to make sure that no previous disease-causing lymphocytes are accidentally included.

We're taking away immunologic memory, Dr. Freedman said. The new immune system is learning all over again what it should and shouldn't be doing.

He said that while the procedure is only done in patients who have not fared well with drug therapy, the best timing for this treatment would be as early as possible, when disability is minimal.

Probably doing it within five years from the onset of illness would give the optimal results, he said.

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Lab-Grown Blood Stem Cells Produced at Last – Scientific American

Posted: May 19, 2017 at 5:48 am

After 20 years of trying, scientists have transformed mature cells into primordial blood cells that regenerate themselves and the components of blood. The work, described today inNature, offers hope to people with leukaemia and other blood disorders who need bone-marrow transplants but cant find a compatible donor. If the findings translate into the clinic, these patients could receive lab-grown versions of their own healthy cells.

One team, led by stem-cell biologist George Daley of Boston Childrens Hospital in Massachusetts, created human cells that act like blood stem cells, although they are not identical to those found in nature. A second team, led by stem-cell biologist Shahin Rafii of Weill Cornell Medical College in New York City, turned mature cells from mice into fully fledged blood stem cells.

For many years, people have figured outparts of this recipe, but theyve never quite gotten there, says Mick Bhatia, a stem-cell researcher at McMaster University in Hamilton, Canada, who was not involved with either study. This is the first time researchers have checked all the boxes and made blood stem cells.

Daleys team chose skin cells and other cells taken from adults as their starting material. Using a standard method, they reprogrammed the cells intoinduced pluripotent stem (iPS) cells, which are capable of producing manyother cell types. Until now, however, iPS cells have not been morphed into cells that create blood.

The next step was the novel one: Daley and his colleagues inserted seven transcription factorsgenes that control other genesinto the genomes of the iPS cells. Then they injected these modified human cells into mice to develop. Twelve weeks later, the iPS cells had transformed into progenitor cells capable of making the range of cells found in human blood, including immune cells. The progenitor cells are tantalizingly close to naturally occurring haemopoetic blood stem cells, says Daley.

Bhatia agrees. Its pretty convincing that George has figured out how to cook up human haemopoetic stem cells, he says. That is the holy grail.

By contrast, Rafiis team generated true blood stem cells from mice without the intermediate step of creating iPS cells. The researchers began by extracting cells from the lining of blood vessels in mature mice. They then inserted four transcription factors into the genomes of these cells, and kept them in Petri dishes designed to mimic the environment inside human blood vessels. There, the cells morphed into blood stem cells and multiplied.

When the researchers injected these stem cells into mice that had been treated with radiation to kill most of their blood and immune cells, the animals recovered. The stem cells regenerated the blood, including immune cells, and the mice went on to live a full lifemore than 1.5 years in the lab.

Because he bypassed the iPS-cell stage, Rafii compares his approach to a direct aeroplane flight, and Daleys procedure to a flight that takes a detour to the Moon before reaching its final destination. Using the most efficient method to generate stem cells matters, he adds, because every time a gene is added to a batch of cells, a large portion of the batch fails to incorporate it and must be thrown out. There is also a risk that some cells will mutate after they are modified in the lab, and could form tumours if they are implanted into people.

But Daley and other researchers are confident that the method he used can be made more efficient, and less likely to spur tumour growth and other abnormalities in modified cells. One possibility is to temporarily alter gene expression in iPS cells, rather than permanently insert genes that encode transcription factors, says Jeanne Loring, a stem-cell researcher at the Scripps Research Institute in La Jolla, California. She notes that iPS cells can be generated from skin and other tissue that is easy to access, whereas Rafiis method begins with cells that line blood vessels, which are more difficult to gather and to keep alive in the lab.

Time will determine which approach succeeds. But the latest advances have buoyed the spirits of researchers who have been frustrated by their inability to generate blood stem cells from iPS cells. A lot of people have become jaded, saying that these cells dont exist in nature and you cant just push them into becoming anything else, Bhatia says. I hoped the critics were wrong, and now I know they were.

This article is reproduced with permission and wasfirst publishedon May 17, 2017.

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Researchers develop a more precise and controlled method of … – Phys.Org

Posted: May 19, 2017 at 5:48 am

May 15, 2017 Near-infrared light is used to precisely engineer stem cells into tissue. Credit: University of California - Santa Barbara

Nothing beats nature. The diverse and wonderful varieties of cells and tissues that comprise the human body are evidence of that.

Each one of us starts out as a mass of identical, undifferentiated cells, and thanks to a combination of signals and forces, each cell responds by choosing a developmental pathway and multiplying into the tissues that become our hearts, brains, hair, bones or blood. A major promise of studying human embryonic stem cells is to understand these processes and apply the knowledge toward tissue engineering.

Researchers in UC Santa Barbara's departments of Chemistry and Biochemistry, and of Molecular, Cellular and Developmental Biology have gotten a step closer to unlocking the secrets of tissue morphology with a method of three-dimensional culturing of embryonic stem cells using light.

"The important development with our method is that we have good spatiotemporal control over which cellor even part of a cellis being excited to differentiate along a particular gene pathway," said lead author Xiao Huang, who conducted this study as a doctoral student at UCSB and is now a postdoctoral scholar in the Desai Lab at UC San Francisco. The research, titled "Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells," appears in volume 28, issue 48 of the journal Advanced Materials.

Similar to other work in the field of optogeneticswhich largely focuses neurological disorders and activity in living organisms, leading to insights into diseases and conditions such as Parkinson's and drug addictionthis new method relies on light to control gene expression.

The researchers used a combination of hollow gold nanoshells attached to small molecules of synthetic RNA (siRNA)a molecule that plays a large role in gene regulationand thermoreversible hydrogel as 3D scaffolding for the stem cell culture, as well as invisible, near-infrared (NIR) light. NIR light, Huang explained, is ideal when creating a three-dimensional culture in the lab.

"Near-infrared light has better tissue penetration that is useful when the sample becomes thick," he explained. In addition to enhanced penetrationup to 10 cm deepthe light can be focused tightly to specific areas. Irradiation with the light released the RNA molecules from the nanoshells in the sample and initiated gene-silencing activity, which knocked down green fluorescent protein genes in the cell cluster. The experiment also showed that the irradiated cells grew at the same rate as the untreated control sample; the treated cells showed unchanged viability after irradiation.

Of course, culturing tissues consisting of related but varying cell types is a far more complex process than knocking down a single gene.

"It's a concert of orchestrated processes," said co-author and graduate student researcher Demosthenes Morales, describing the process by which human embryonic stem cells become specific tissues and organs. "Things are being turned on and turned off." Perturbing one aspect of the system, he explained, sets off a series of actions along the cells' developmental pathways, much of which is still unknown.

"One reason we're very interested in spatiotemporal control is because these cells, when they're growing and developing, don't always communicate the same way," Morales said, explaining that the resulting processes occur at different speeds, and occasionally overlap. "So being able to control that communication on which cell differentiates into which cell type will help us to be able to control tissue formation," he added.

The fine control over cell development provided by this method also allows for the three-dimensional culture of tissues and organs from embryonic stem cells for a variety of applications. Engineered tissues can be used for therapeutic purposes, including replacements for organs and tissues that have been destroyed due to injury or disease. They can be used to give insight into the body's response to toxins and therapeutic agents.

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Researchers develop a more precise and controlled method of ... - Phys.Org

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ASU engineers envision ‘guiding’ body’s cells to help improve health – Arizona State University

Posted: May 19, 2017 at 5:47 am

May 17, 2017

Much of what happens inside the human body at its most basic biological level is determined by how its cells transition from one state to another.

In particular, stem cells the primal cells common to all multicellular organisms have the ability to divide and differentiate into a range of specialized kinds of cells that are essential to critical bodily systems and functions. Some cells can even change to different kinds of cells more than once. The technology used in Xiao Wangs Systems and Synthetic Biology Lab can capture images of cellular fluorescence in microfluidic devices. The images are used to illustrate in real time how engineered E. coli cells transition from one state or function to another. Photo by Jessica Hochreiter/ASU Download Full Image

What is still unknown to a significant degree are all the factors within the bodys genetic environment that determine what kinds of specialized cells that the unspecialized stem cells will transition into.

A thorough understanding of what controls the cell differentiation process would unlock secrets to guiding cell fates, and open up the potential for developing more and better cell-based medical therapies, according to Xiao Wang.

Wang is an associate professor of biomedical engineering in Arizona State Universitys Ira A. Fulton Schools of Engineering and directs the Systems and Synthetic Biology Lab.

HIs lab team and faculty collaborators have been trying to fill in missing pieces of the picture presented by Waddingtons epigenetic landscape, a visualization of pathways a cell might follow toward differentiation.

Scientist Conrad Hal Waddingtons illustration of a biological landscape of ridges and valleys that a cell could move along on its way to differentiation was a valuable step forward in genetics in the mid-20th century.

With the benefit of modern advances in mathematical modeling, computer science, bioengineering, physics and molecular biology, Wang and his colleagues have expanded on Waddingtons conceptualization of the determinants of cell fates.

Their conclusions have recently been published in the biomedical and life sciences research journal eLife, in the article Engineering of a synthetic quadrastable gene network to approach Waddington landscape and cell fate determination.

Biomedical engineering doctoral student Fuqing Wu conducted the experiments to test Wangs theories about the mechanisms of cell differentiation. Photo by Jessica Hochreiter/ASU

Biomedical engineering doctoral student Fuqing Wu performed the experiments for the research project and postdoctoral research associate Ri-Qi Su developed the mathematical modeling.

Wangs chief partner on the project was Ying-Cheng Lai, a Fulton Schools professor of electrical, computer and energy engineering.

The eLife journal editors write that the team has successfully charted how the environment in which cell fates are altered will change in the presence of various chemicals, and that cells transitions can be guided by introducing certain chemicals into that landscape in specifically ordered sequences.

Their work helps us understand how multiple cell fates may be achieved and how we might manipulate cell fate transitions, the editors write.

The research not only lays a theoretical foundation for how cell differentiation could be controlled, Wang explained, but also provides results of experimentation to support the theory.

The upshot is that by changing the order in which mixtures of particular chemicals and protein molecules are introduced into the environment, one kind of cell can be manipulated into turning into other specific kinds of cells.

With the ability to do that, he envisions being able to someday control the mechanisms that determine cell fates for the purposes of treating infections and diseases, and repairing body tissues and organs.

Associate Professor Xiao Wang says the most dramatic impact of learning to manipulate the cell transition process might be reducing the need for organ transplants. Photo by Jessica Hochreiter/ASU

He sees potential uses for improving therapies and treatments for Alzheimers disease, spinal injuries and even blindness.

The most dramatic impact could be on reducing the need for transplants.

When tissues or organs are badly damaged, the only option we often have today are transplants. We have to take parts from other bodies, he said. But by turning our own cells into the kinds of cells needed to produce specific types of tissues, we might be able to generate that new tissue from our own genetic material.

Getting to that point will take much more research and experimentation, Wang says, and he plans for his lab to pursue answers to more complex questions about activating cell differentiation and how to best take of advantage of controlling the process.

We dont want to over-claim about what might be achieved, Wang said. But what we are learning, and the possibilities it raises, is very exciting.

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GV Gardeners: Sun-loving saguaro Southwest symbol | Sun Life … – Sahuarita Sun (subscription)

Posted: May 19, 2017 at 5:47 am

A large, well-hydrated saguaro can weigh more than 10 tons! This native cactus is protected in Arizona by regulations restricting the harvest or sale of wild saguaros. However, seed-grown plants are readily available from commercial nurseries.

Currently starting its annual bloom season, the saguaro became the official state flower of Arizona in 1931. Although portrayed in movies and advertisements throughout the Southwest, it grows only in Southern Arizona and western Sonora, Mexico.

First, a little anatomy the exterior of the saguaro is covered with a thick, waxy skin that waterproofs the surface and restricts loss of water. Just beneath this layer is a thin layer of chlorophyll-containing cells.

Outer pleats enable the stem to expand without bursting during water uptake. Clusters of hard spines along the pleats provide shade for the surface, reducing heat load and water loss. The deeper interior consists of water storage tissue.

Water makes up 75 to 85 percent of the weight of a saguaro. The retained water helps prevent temperature extremes which are harmful to the plant. A skeleton of 12 to 20 woody ribs is in the center of the stem, running through the main stem and branching into the arms. Surprisingly, roots for this giant are rarely more than 4 inches deep, radiating horizontally from the plant as far as it is tall.

White flowers open late at night and remain open until the next mid-day, releasing an aroma much like an over-ripe melon. Pollination takes place both at night when bats feed on the nectar, and during the day when bees and white-winged doves feast.

During June, the pollinated flowers mature into 3-inch fruit containing many tiny seeds embedded in the juicy, red pulp. When the rind splits and displays the bright inner lining, the open fruit is often mistaken for red flowers.

Saguaro fruit ripens during pre-monsoon drought and is often the only moist food available for wildlife. It becomes a staple for many insects, birds and mammals. Conveniently, seed dispersal takes place just prior to the summer rainy season.

From a seed the size of a pinhead, successful sprouting takes place under the protection of another plant, referred to as the nurse plant. In 10 years the plant grows to 1.5 inches high. If it survives for 30 years, the saguaro reaches 2 feet high. By 50 years, most plants flower, produce arms, and may top out at 8 to 10 feet high.

Some saguaros may have as many as 50 arms; many will never grow any arms. Studies have shown that arm production is random. Saguaro arms grow upward. Drooping or twisted arms are caused by wilting after freeze damage.

Whether with or without arms, the saguaro is a well-engineered, statuesque, sun-loving symbol of the Southwest desert and the state of Arizona.

Mary Kidnocker is a University of Arizona Master Gardener who lives in Green Valley. Her articles are featured weekly.

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