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Woodrose Ventures Corporation Announces Proposed Acquisition of Global Stem-Cell Biotechnology Company … – Marketwired (press release)

Posted: March 13, 2017 at 8:43 pm

VANCOUVER, BRITISH COLUMBIA--(Marketwired - March 13, 2017) -

NOT FOR DISSEMINATION IN THE UNITED STATES

Editors Note: There is a photo associated with this press release.

Woodrose Ventures Corporation (TSX VENTURE:WRS.H) ("Woodrose" or the "Company") is pleased to announce that it has entered into an agreement (the "Agreement") dated March 10, 2017 to acquire all of the shares of Novoheart Holdings Ltd. ("Novoheart"), a global stem cell biotechnology company dedicated to human heart engineering (the "Transaction"). Novoheart develops products and provides services focused on engineering prototypes of bio-artificial human heart tissues and chambers for drug discovery, cardiotoxicity screening, disease modelling and therapeutic applications.

The Transaction will constitute a "reverse-takeover" of Woodrose in accordance with the policies of the TSX Venture Exchange (the "TSXV") and the reactivation of Woodrose, which is currently a NEX-listed issuer.

About Novoheart

Novoheart is a global stem cell biotechnology company headquartered in Hong Kong with R&D Innovation Centres being set up in the United States. Novoheart's mission is to revolutionize drug discovery and the development of heart therapeutics with its range of proprietary bioengineered human heart constructs, collectively known as the MyHeart platform, and to further develop them into transplantable heart grafts for cell-based regenerative therapies with superior safety and efficacy. Its scientific team has pioneered a range of best-in-class bioengineering technologies and constructed the world's first human mini-heart "novoHeart" with which the Novoheart team intends to revolutionize:

1) Pre-clinical drug discovery, cardiotoxicity screening and heart disease modelling;

2) Post-discovery, clinical development of novel therapeutics; and

3) Pre-clinical and clinical development of cell-based cardiac regenerative therapies.

Novoheart's immediate focus is to innovate and accelerate the lengthy, expensive and inefficient drug development process. The development of a new drug candidate typically costs US$2-4bn and takes 10+ years (Tufts Centre for the Study of Drug Development, Tufts CSDD R&D Cost Study 2014) with extremely poor success rates of

Novoheart's intellectual property portfolio, including the human "heart-in-a-jar" (novoHeart) and other related next-generation technologies of the MyHeart platform (see figure below) are unique solutions that help bridge the gap between pre-clinical and clinical drug trials. The MyHeart platform provides advanced human heart surrogates for pre-screening of drug formulas and the elimination of toxic compounds early on in the drug development process, minimizing the risk towards patients. Significantly, the MyHeart Platform provides real time data on the effects of drug formulations enabling drug development companies to undertake "on-the-fly" reformulation of drug candidates to optimize efficacy and toxicological profiles. With Novoheart's technologies, we aim to significantly reduce pre-clinical R&D time and costs, and importantly, improve trial successes. It is anticipated that drug screening results using Novoheart's human engineered tissues would be accepted as reliable indicators for toxicity and efficacy, thereby qualifying the test compounds for accelerated drug development.

Novoheart adopts a hybrid business model by:

These products and services are designed to significantly reduce the time, cost, and use of animal models, as well as improve patient safety, and facilitate pharmaceutical discovery and development. Novoheart is currently working with leading academic and pharmaceutical partners to innovate drug discovery and toxicity screening protocols. Our targeted clients are pharmaceutical companies, government units, and research institutions.

Novoheart was incorporated in 2014 pursuant to the laws of British Virgin Islands (BVI) and its controlling shareholder is Medera Group Limited, a BVI entity. Novoheart has one wholly owned Hong Kong subsidiary "Novoheart Limited" ("Novoheart Hong Kong") which is the group operating entity.

Novoheart Hong Kong was incorporated in January 2014 by founder and CEO Prof. Ronald Li, with scientific co-founders Prof. Kevin Costa and Prof. Michelle Khine.

Novoheart's foundational technologies are the direct outcome of over 15 years of research effort supported by R&D investments amounting to approximately USD30MM. These research efforts, performed at Johns Hopkins University, Icahn School of Medicine at Mount Sinai, University of California Irvine, University of California Davis, and the University of Hong Kong by our scientific founders, have received major recognitions such as American Heart Association's Best Study of 2005, Ground-breaking Study of 2006, and Late-breaking Studies of 2002, 2003, 2005 and 2007, and the Spirit of Hong Kong Innovating for Good Award in 2015. The "human-heart-in-a-jar" technology was selected by Google's Solve For X as a Moonshot Project in 2015.

Novoheart's scientific founders and advisors are renowned pioneering leaders in the stem cell and cardiac space, with a successful track record in developing and commercializing ground-breaking technologies. In September 2014, Novoheart established its R&D base and office in the Hong Kong Science Park, where it continues to innovate solutions for drug discovery and human heart tissue engineering.

In December 2014, Novoheart signed a strategic partnership with a major global pharmaceutical company (the "Global Pharma Partner") headquartered in New York City to validate the MyHeart platform. The success of this validation process has resulted in follow on income-generating projects.

In January 2015, Novoheart's R&D proposal to develop bio-artificial heart tissues for drug screening received 50/50 matched funding from the Innovation & Technology Commission (ITC) of the Government of Hong Kong, with a total project cost of over HK$21MM over 2 years. It was also the largest biotech project granted by ITC for that year. Novoheart owns all of the intellectual property generated from this project, and as a result of the R&D, Novoheart has applied or is in the application process for 3 new patents covering newly developed technology, including the human ventricular cardiac anisotropic sheet (hvCAS) as a powerful tool for detecting drug-induced arrhythmias with the results published in the prestigious international peer-reviewed bioengineering journal Advanced Materials (Shum et al. 2017, Advanced Materials, 29). Additionally, Novoheart holds exclusive worldwide licenses or options to acquire the same for technologies that constitute its MyHeart platform and future developments.

In December 2015, Novoheart signed a second contract with the Global Pharma Partner to build disease-specific engineered human heart tissues and chambers for drug discovery. The total project cost is US$726,000 over 1.5 years.

In February 2017, the Corporate Venture Fund (CVF) of the Hong Kong Science and Technology Parks Corporation (HKSTPC) completed an equity investment of approximately US$250,000 into Novoheart and an additional investment would be made at the Transaction.

Novoheart Financial Information

The following table includes a summary of certain financial information of Novoheart and is derived from its financial statements for the years ended June 30, 2016 and June 30, 2015.

Summary of the Transaction

Under the terms of the Agreement, the shareholders of Novoheart will receive an aggregate of 66,086,600 common shares of Woodrose on a post-Consolidation basis (see below) ("Woodrose Post-Consolidation Shares"). In addition, a finder's fee of 2,313,038 Woodrose Post-Consolidation Shares will be paid to Cynosure Private Equity Limited in connection with the Transaction.

In connection with the Transaction, Woodrose intends to complete a consolidation of all its outstanding common shares on the basis of 3.56878449 old common shares for each one new common share (the "Consolidation"). In addition, Woodrose intends to complete a non-brokered private placement (the "Private Placement") of 11,700,000 subscription receipts ("Subscription Receipts") at a price of CDN$0.50 per Subscription Receipt to raise gross proceeds of CDN$5,850,000, which will be held in escrow in accordance with the terms of a subscription receipt agreement (the "Subscription Receipt Agreement"). It is anticipated that the Subscription Receipt Agreement will provide that, upon completion of the Transaction, each Subscription Receipt will automatically convert into one Woodrose Post-Consolidation Share. The Subscription Receipt Agreement will also provide that, in the event the Transaction is terminated or does not complete within an agreed timeframe, the Subscription Receipts will be cancelled and the funds will be returned to the holders. Woodrose may pay cash fees in an amount not to exceed 7% of the gross proceeds (to a maximum of $364,000) to certain finders involved in the Private Placement and may issue finder's warrants ("Finder's Warrants"), in an amount not to exceed 7% of the number of Subscription Receipts issued (to a maximum of 728,000 Finders Warrants) each of which would entitle the holder to acquire one Woodrose Post-Consolidation Share at a price of CDN$0.50 for a period of two years following closing of the Private Placement. All securities issued pursuant to the Private Placement will be subject to a statutory hold period of four months and one day.

The Company intends to use the net proceeds of the offering to finance investment in drug discovery and screening, establish commercial partnerships, expand the current laboratory, hire additional research and development team members and for working capital and general corporate purposes.

Upon completion of the Transaction, it is anticipated that the Company will be classified as a Tier 2 Technology Issuer on the TSXV and will change its name to "Novoheart Holdings (BC) Limited" or such other name as is acceptable to the Board of Directors. Closing of the Transaction ("Closing") is subject to conditions precedent, that include, but are not limited to, the following:

The Transaction is an "arm's length" transaction (as defined by the policies of the TSXV). Woodrose intends to rely an exemption from the sponsorship requirements of the policies of the TSXV.

Proposed Management Team

Upon closing of the Transaction, the following directors and senior officers are anticipated to be appointed in replacement of Woodrose's current board and management:

Prof. Ronald Li, B.Sc. (Hons), Ph.D. (Proposed President, Chief Executive Officer and Director)

Prof. Ronald Li is a co-founder of Novoheart, and has been serving as the CEO since 2016. He is concurrently Director of Ming-Wai Lau Centre for Reparative Medicine, HK node, Karolinska Institutet (KI), Sweden, with a professorial cross appointment at the Dr. Li Dak-Sum Research Centre, The University of Hong Kong (HKU)-KI Collaboration in Regenerative Medicine of HKU. Prof. Li has been an advocate of stem cell technology for many years, starting from his career as Assistant Professor of Cardiology, and Cellular and Molecular Medicine at the Johns Hopkins University (JHU) School of Medicine. He founded and led the Human Embryonic Stem Cell Consortium when he was recruited in 2005 to become a tenured Associate Professor at the University of California, Davis, in light of state's USD3-billion stem cell initiative Proposition 71. Prof. Li was the Founding Director of the Stem Cell & Regenerative Medicine Consortium (SCRMC) at the University of Hong Kong (HKU) from 2010 to 2015. He also co-directed the Section of Cardiovascular Cell & Tissue Engineering in Icahn School of Medicine at Mount Sinai with Prof. Kevin Costa. Prof. Li has received multiple accolades and recognitions during his career, including the Spirit of Hong Kong Innovating for Good Award by the South China Morning Post (2015), the Top Young Faculty Award (2002, 2004), the Top Prize for the Young Investigator Basic Research (2001) and Top Postdoctoral Fellow Helen Taussig Award (2001) of JHU School of Medicine, Young Investigator Award 1st Prize from the Heart Rhythm Society (2002), and the Career Development Award from the Cardiac Arrhythmias Research & Education Foundation (2001).

Prof. Li graduated with his B.S. with honors in Biotechnology from University of Waterloo, Ontario, on Dean's List and his Ph.D. in Cardiology/Physiology at the University of Toronto.

Dr. Camie Chan, B.Sc. (Hons), M.Sc., Ph.D. (Proposed Chief Operating Officer and Director)

Dr. Camie Chan joined Novoheart Hong Kong as the Chief Operating Officer in 2016, after having served at HKU as the Deputy Director of the Faculty of Medicine Core Facility, a founding member of the Management Committee of the Stem Cell & Regenerative Medicine Consortium (SCRMC), and Assistant Professor in the Department of Anatomy, between 2010 and 2016. She has had extensive experience managing laboratory operations in her capacity at HKU, and her prior career as Assistant Professor at the University of California, Davis, and Assistant Investigator at the Shriners Hospital for Children. Dr. Chan is also a co-inventor of technology allowing mass production of human ventricular heart cells from pluripotent stem cells.

Dr. Chan graduated with her B.Sc. with honors at the University of Waterloo, followed by obtaining her M.Sc. degree in Medical Sciences and Ph.D. degree in Immunology at the University of Toronto, Canada. She then received postdoctoral training at the Sydney Kimmel Cancer Research Center at the Johns Hopkins University. She has garnered numerous awards in her career, including the prestigious National Institute of Allergy and Infectious Diseases (NIAID) Developmental Research Grant Award.

Prof. Kevin Costa, B.S., Ph.D. (Proposed Chief Scientific Officer)

Prof. Costa is Director of the Section of Cardiovascular Cell and Tissue Engineering at the Icahn School of Medicine at Mount Sinai in New York City. Prof. Costa was previously trained at the Johns Hopkins University and on the faculty as Associate Professor of Biomedical Engineering at Columbia University. As a "blue-blood" biomedical engineering (BME) expert (B.S. and M.S. in BME from Boston University, Ph.D. in BME from UC San Diego, and postdoc in BME from JHU and University of Washington) in cell and tissue biomechanics and cardiac tissue engineering, he has developed one of the first engineered cardiac tissue systems. Since 2009, he has been working with Prof. Ronald Li to translate such systems into human cells. Prof. Costa has received research funding from the Whitaker Foundation, the National Science Foundation (NSF) and the National Institutes of Health (NIH; NHLBI, NIBIB, and NIGMS). He was also a recipient of the prestigious Faculty Early Career Development (CAREER) Award from the NSF. Prof. Costa is an inventor of several cardiac tissue engineering technologies and one of the scientific co-founders of Novoheart Hong Kong.

Ms. Iris Lo, B. Comm. (Hons), CPA, CA (Proposed Chief Financial Officer)

Ms. Lo is a seasoned professional with expertise in corporate finance, mergers and acquisitions, accounting, and finance. Prior to joining Novoheart, Ms. Lo was the Director of Corporate Development & Analysis at Cardiome Pharma Corp., a Canadian public company dually listed on the TSX and NASDAQ (TSX: COM, NASDAQ: CRME). At Cardiome, she held responsibilities in equity and debt financing, corporate mergers and acquisitions, product licensing and distributions, financial planning and analysis, as well as regulatory and risk management. During her tenure at Cardiome, Ms. Lo participated in transactions totaling over US$240 million as Cardiome grew from a company with a market capitalization of US$25 million to over US$150 million at its peak. She brings with her valuable experience from the life sciences and pharmaceutical sector, as well as expertise in dealing with the complexities of operating and financing public corporations. Ms. Lo was also previously a Manager in the Transaction Services team at PwC Hong Kong and began her career articling with KPMG Vancouver. She is a Chartered Professional Accountant and holds a Bachelor of Commerce (Honours) from the Sauder School of Business at the University of British Columbia.

Mr. Victor Chang (Proposed Director)

Mr. Chang is a seasoned investor who has lately become focused on start-ups. Mr. Chang started his career with Lippo Securities Limited in 1996 and became a Director of Grand International Holdings Limited in 1999, which was engaged in general investments. During the period from 2007 to 2009, he was a Director and Responsible Officer for Astrum Capital Management Limited carrying out regulated activities under the Securities and Futures Ordinance ("SFO", Cap. 571, Laws of Hong Kong) and with Murtsa Capital Partners Limited as well. During the period from 2007 to 2012, he was also a compliance consultant for Astrum Capital Management Limited. As co-founder and Managing Director of Zebra Strategic Outsource Solution, he has over 16 years of experience in recruitment process outsourcing, executive search as well as and private investment management. In Apil 2013, he successfully brought Zebra Strategic Holdings Limited which offers holistic HR solutions to IPO on the HK GEM board (Stock Code: 8260) and was re-designated as and is currently a Non-Executive Director with the company. He is currently a Director and Responsible Officer of Dakin Financial Group, a corporation licensed to carry out type 1, 2 & 9 regulated activity under the Hong Kong Securities and Futures Ordinance.

Mr. Tong Ricky Chiu (Proposed Director)

As a key founder and visionary for Grand Power Logistics Group Inc., which was listed on the TSX Venture Exchange (GPW.V) before its privatization in 2016, and Baoshinn International Express Ltd., Mr. Chiu adds value with his immense corporate development and growth skills. He received his education in Oxford University, England, and Beijing University, and began his career in Australia. He has a diversified background in a wide range of industries with roles in finance, audit, real estate, merchandise trading and travel, as well as logistics.

Mr. James Topham (Proposed Director)

Mr. Topham is an experienced executive with expertise in finance, accounting, auditing and entrepreneurial technology companies. He was an audit partner leading KPMG's Technology Group in the Vancouver office for 20 years where he worked with many fast growing public companies and was involved in many M&A and IPO transactions in Canada, the US and Europe. Mr. Topham founded Social Venture Partners Vancouver in 2001 with a mission to strengthen the organizational capacity of innovative non-profits serving children in-need and youth at-risk. It has funded several million dollars and provided thousands of hours of executive time mentoring these local non-profits. Since retiring at KPMG 7 years ago, Mr. Topham has worked on several Boards of both public and private technology companies. He received a lifetime achievement award from the BC Technology Industry Association and was awarded the designation of Fellow Chartered Public Accountant (FCPA) from the Chartered Public Accountants of BC for his career achievements in the profession and community. He was a founder and Board member for 9 years of the BC Technology Industry Association that represents the technology industry in BC. Mr. Topham is a CPA and has a Bachelor of Commerce degree with Honours from the University of Saskatchewan graduating as the most distinguished graduate in the College of Commerce.

Mr. Allen Ma (Proposed Director)

As a 30-year technology industry veteran, Mr. Ma was the CEO of Hong Kong Science & Technology Parks before he retired in July 2016. He held senior executive positions within the information and communications technology sector. His past roles include president for Asia-Pacific at British Telecom, vice-president for Asia at the global telecom solutions sector of Motorola, executive director of Hong Kong Telecommunications - subsequently called Cable & Wireless HKT - and managing director of Hong Kong Telecom CSL. Ma holds an MBA from the University of Toronto and is a fellow member of both the Chartered Institute of Management Accountants, UK and the Association of Chartered Certified Accountants, UK. He is also a Certified Management Accountant of Canada.

Proposed Advisory Team

Novoheart is supported by a Scientific Advisory Board whose proposed composition consists of eminent scientists renowned in the fields of stem cells, cardiac biology and physiology, tissue engineering, and clinical cardiology including clinical trials research, from top academic research institutes in the U.S.A. Their technical expertise will guide the development of Novoheart as a forerunner in the application of cutting-edge technologies to develop new and better treatments for heart disease and beyond.

Further Details

Both the Company and Novoheart intend to work diligently to complete the conditions precedent to Closing and anticipate completion of the Transaction in the second quarter of 2017. The Company will update its shareholders with further details as they become available.

ON BEHALF OF WOODROSE VENTURES CORPORATION

Darren Devine, President, CEO and Director

NEITHER THE TSX VENTURE EXCHANGE NOR ITS REGULATION SERVICES PROVIDER (AS THAT TERM IS DEFINED IN THE POLICIES OF THE TSX VENTURE EXCHANGE) ACCEPTS RESPONSIBILITY FOR THE ADEQUACY OR ACCURACY OF THIS RELEASE.

Completion of the Transaction is subject to a number of conditions, including but not limited to, Exchange acceptance and if applicable pursuant to Exchange requirements, majority shareholder approval. Where applicable, the Transaction cannot close until the required shareholder approval is obtained. There can be no assurance that the Transaction will be completed as proposed or at all.

Investors are cautioned that, except as disclosed in the Filing Statement to be prepared in connection with the Transaction, any information with respect to the Transaction may not be accurate or complete and should not be relied on. Trading in securities of the Company should be considered highly speculative.

The TSX Venture Exchange has in no way passed upon the merits of the Transaction and has neither approved nor disproved the contents of this news release.

Cautionary Note Regarding Forward-Looking Statements

Information set forth in this news release may involve forward-looking statements under applicable securities laws. Forward-looking statements are statements that relate to future, not past, events. In this context, forward-looking statements often address expected future business and financial performance, and often contain words such as "anticipate", "believe", "plan", "estimate", "expect", and "intend", statements that an action or event "may", "might", "could", "should", or "will" be taken or occur, or other similar expressions. All statements, other than statements of historical fact, included herein including, without limitation; statements about the terms and completion of the Transaction are forward-looking statements. By their nature, forward-looking statements involve known and unknown risks, uncertainties and other factors which may cause the actual results, performance or achievements, or other future events, to be materially different from any future results, performance or achievements expressed or implied by such forward-looking statements. Such factors include, among others, the following risks: failure to satisfy all conditions precedent to the Transaction, including shareholder approval, approval of the TSX Venture Exchange and completion of the necessary financings and the additional risks identified in the management discussion and analysis section of Woodrose Corporation's interim and most recent annual financial statement or other reports and filings with the TSX Venture Exchange and applicable Canadian securities regulators. Forward-looking statements are made based on management's beliefs, estimates and opinions on the date that statements are made and the respective companies undertakes no obligation to update forward-looking statements if these beliefs, estimates and opinions or other circumstances should change, except as required by applicable securities laws. Investors are cautioned against attributing undue certainty to forward-looking statements.

To view the photo associated with this press release, please visit the following link: http://www.marketwire.com/library/20170312-1088577_MyHeart_800.jpg

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Woodrose Ventures Corporation Announces Proposed Acquisition of Global Stem-Cell Biotechnology Company ... - Marketwired (press release)

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Vitamin C can target and kill cancer stem cells, study shows … – Medical News Today

Posted: March 13, 2017 at 8:43 pm

Cancer is currently one of the top killers worldwide, and the number of cancer cases is only expected to rise. Although there are a number of therapies available, most of them are toxic and cause serious side effects. New research examines the impact of the natural vitamin C on cancer cell growth.

Cancer is the second leading cause of death and disease worldwide, accounting for almost 9 million deaths in 2015, according to the World Health Organization (WHO).

The global number of new cases of cancer are expected to grow by around 70 percent in the next 20 years.

In the United States, the National Cancer Institute (NCI) estimate that almost 40 percent of U.S. men and women will have developed cancer at one point during their lives.

There are various treatment options available for cancer, but they are not always effective; most of them are toxic, and they tend to have a variety of side effects.

In some more aggressive cases, the cancer does not respond to treatment, and it is believed that cancer stem-like cells are the reason why the cancer comes back and metastasizes.

New research, published in the journal Oncotarget, examines the effectiveness of three natural substances, three experimental drugs, and one clinical drug in stopping the growth of these cancer stem cells (CSCs.)

The study was conducted by researchers from the University of Salford in Manchester in the United Kingdom, and was led by Dr. Gloria Bonuccelli.

In total, the researchers measured the impact of seven substances: the clinical drug stiripentol, three experimental drugs (actinonin, FK866, and 2-DG), and three natural substances (caffeic acid phenyl ester (CAPE), silibinin, and ascorbic acid (vitamin C).)

The research focused on the bioenergetic processes of CSCs, which enable the cells to live and multiply. The study aimed to disrupt the CSCs' metabolism and ultimately prevent their growth.

Of all the substances tested, the team found that actinonin and FK866 were the most effective. However, the natural products were also found to prevent the formation of CSCs, and vitamin C was 10 times more effective than the experimental drug 2-DG.

Additionally, the study revealed that ascorbic acid works by inhibiting glycolysis - the process by which glucose is broken down within the cell's mitochondria and turned into energy for the cell's proliferation.

Dr. Michael P. Lisanti, professor of translational medicine at the University of Salford, comments on the findings:

"We have been looking at how to target cancer stem cells with a range of natural substances including silibinin (milk thistle) and CAPE, a honey-bee derivative, but by far the most exciting are the results with vitamin C. Vitamin C is cheap, natural, nontoxic and readily available so to have it as a potential weapon in the fight against cancer would be a significant step."

"This is further evidence that vitamin C and other nontoxic compounds may have a role to play in the fight against cancer," says the study's lead author.

"Our results indicate it is a promising agent for clinical trials, and as an add-on to more conventional therapies, to prevent tumor recurrence, further disease progression, and metastasis," Bonuccelli adds.

Vitamin C has been shown to be a potent, nontoxic, anticancer agent by Nobel Prize winner Linus Pauling. However, to the authors' knowledge, this is the first study providing evidence that ascorbic acid can specifically target and neutralize CSCs.

Learn how 300 oranges' worth of vitamin C can impair cancer cells.

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Vitamin C can target and kill cancer stem cells, study shows ... - Medical News Today

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Some genetic variations difficult to evaluate using current stem cell modeling techniques – Phys.Org

Posted: March 13, 2017 at 8:42 pm

March 13, 2017

Some heritable but unstable genetic mutations that are passed from parent to affected offspring may not be easy to investigate using current human-induced pluripotent stem cell (hiPSC) modeling techniques, according to research conducted at The Icahn School of Medicine at Mount Sinai and published March 14, in the journal Stem Cell Reports. The study serves to caution stem cell biologists that certain rare mutations, like the ones described in the study, are difficult to recreate in laboratory-produced stem cells.

Stem cell-based disease modeling involves taking cells from patients, such as skin cells, and introducing genes that reprogram the cells into human-induced pluripotent stem cells (hiPSCs). These "master cells" are unspecialized, meaning they can be pushed to become any type of mature cell needed for research, such as skin, liver or brain. The hiPSCs are capable of renewing themselves over a long period of time, and this emerging stem cell modeling technique is helping elucidate the genetic and cellular mechanisms of many different disorders.

"Our study describes how a complex chromosomal rearrangement genetically passed by a patient with psychosis to her affected son was not well recreated in laboratory-produced stem cells," says Kristen Brennand, PhD, Associate Professor of Genetics and Genomic Sciences, Neuroscience, and Psychiatry at the Icahn School of Medicine, and the study's senior investigator. "As stem cell biologists dive into studying brain disorders, we all need to know that this type of rare mutation is very hard to model with induced stem cells."

To investigate the genetic underpinnings of psychosis, the research team used hiPSCs from a mother diagnosed with bipolar disease with psychosis, and her son, diagnosed with schizoaffective disorder. In addition to the normal 46 chromosomes (23 pairs), the cells in mother and son had a very small extra chromosome, less than 1/10th normal size. This microduplication of genes is increasingly being linked to schizophrenia and bipolar disorders, and the extra chromosomal bit, known as a marker (mar) element, falls into the category of abnormally duplicated genes.

For the first time, the Mount Sinai research team tried to make stem cells from adult cells with this type of mar defect. Through the process, they discovered that the mar element was frequently lost during the reprogramming process.

While mar elements in the general population are rare (less than .05 percent in newborn infants), more than 30 percent of individuals with these defects are clinically abnormal, and mar elements are also significantly more likely to be found in patients with developmental delays.

The study found that the mother's cells were mosaic, meaning some cells were normal while others were not, and the hiPSCs the team created accurately replicated that condition: some were normal and some had the extra mar chromosome. But the technique did not work well with the son's cells. While all of his cells should have had the mar element, as with his mother, some of the reprogrammed stem cells did not contain the extra bit of chromosome.

"We realized we kept losing the mutation in the stem cells we made, and the inability to recreate cells with mar elements may hamper some neuropsychiatric research," says Dr. Brennand. "The bottom line is that it is essential that stem cell biologists look for existing mar elements in the cells they study, in order to check that they are retained in the new stem cells."

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Researchers have discovered a new efficient way to generate thyroid cells, known as thyrocytes, using genetically modified embryonic stem cells.

A protein that stays attached on chromosomes during cell division plays a critical role in determining the type of cell that stem cells can become. The discovery, made by EPFL scientists, has significant implications for ...

Researchers at the RIKEN Brain Science Institute have used human-induced pluripotent stem cells (hiPSCs) to identify a characteristic of abnormal brain development in schizophrenia. Published in Translational Psychiatry, ...

Oxygen in the air is well known to cause damaging rust on cars through a process known as oxidation. Similarly, a research group at Lund University in Sweden, has now identified that certain cells during embryonic development ...

Induced pluripotent stem cells (iPSCs)adult cells reprogrammed back to an embryonic stem cell-like statemay better model the genetic contributions to each patient's particular disease. In a process called cellular reprogramming, ...

Successful reprogramming of muscle cells derived from biopsies of patients with Andersen's syndrome (AS) led to the formation of induced pluripotent stem (iPS) cells that can serve as a valuable model for understanding the ...

New research by Professor Beth Shapiro of the UC Santa Cruz Genomics Institute and University of Alberta Professor Duane Froese has identified North America's oldest bison fossils and helped construct a bison genealogy establishing ...

Scientists have determined the first 3D structures of intact mammalian genomes from individual cells, showing how the DNA from all the chromosomes intricately folds to fit together inside the cell nuclei.

Scientists have documented many cases in which the timing of seasonal events, such as the flowering of plants or the emergence of insects, is changing as a result of climate change. Now researchers studying a grassland ecosystem ...

Most fish live either in fresh water or salt water, but others, including tilapia, have the remarkable ability to physiologically adjust to varying salinity levelsa trait that may be critically important as climate change ...

When an individual cell needs to move somewhere, it manages just fine on its own. It extends protrusions from its leading edge and retracts the trailing edge to scoot itself along, without having to worry about what the other ...

A subset of protein complexes whose role has long been thought to consist only of chemically degrading and discarding of proteins no longer needed by cells appears to also play a role in sending messages from one nerve cell ...

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Some genetic variations difficult to evaluate using current stem cell modeling techniques - Phys.Org

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More Clarity Emerges on US Stem Cell Inc (OTCMKTS:USRM) – The Oracle Dispatch

Posted: March 13, 2017 at 8:42 pm

US Stem Cell Inc (OTCMKTS:USRM) is a penny player in the stem cell space that recently caught some major air off the pattern lows. We looked at this stock a couple weeks ago, and suggested some theories about the movement, finding no clear signs that USRM stock was being openly pushed by investor awareness activity. Instead, we hypothesized that the blast off was related to the potential amicable settlement of a legal matter withNorthStar Biotech.

At this point, shares are consolidating the recent advance, and the company has come out with some news clarifying things a bit, recently announcing that Greg Knutson, the Manager of NorthStar Biotech, LLC, has joined the Board of Directors of U.S. Stem Cell, Inc. That is particularly interesting given our hypothesis in our last piece. It would appear this announcement suggests we were correct in our framing.

US Stem Cell Inc (OTCMKTS:USRM) trumpets itself as a company committed to the development of effective cell technologies to treat a variety of diseases and injuries. By harnessing the bodys own healing potential, we may be able to reverse damaged tissue to normal function.

U.S. Stem Cells discoveries include multiple cell therapies in various stages of development that repair damaged tissues throughout the body due to injury or disease so that patients may return to a normal lifestyle.

U.S Stem Cell is focused on regenerative medicine. While most stem cell companies use one particular cell type to treat a variety of diseases, U.S Stem Cell utilizes various cell types to treat different diseases. It is our belief that the unique qualities within the various cell types make them more advantageous to treat a particular disease.

According to company materials, US Stem Cell, Inc. (formerly Bioheart, Inc.) is an emerging enterprise in the regenerative medicine / cellular therapy industry. We are focused on the discovery, development and commercialization of cell based therapeutics that prevent, treat or cure disease by repairing and replacing damaged or aged tissue, cells and organs and restoring their normal function. We believe that regenerative medicine / cellular therapeutics will play a large role in positively changing the natural history of diseases ultimately, we contend, lessening patient burdens as well as reducing the associated economic impact disease imposes upon modern society.

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As we noted last time, there is very little clear information out there to be found on the details of the legal matter between NorthStar Biotech, LLC, and US Stem Cell Inc. However, managements perspective on things offers at least some clue as to how this development fits into the larger picture.

We are very pleased that Greg Knutson, a longtime supporter of the U.S. Stem Cell family of companies, has agreed to join our Board and assist us to continue with our recent technological advances and financial accomplishments, said Mike Tomas, President and CEO of U.S. Stem Cell, Inc. A longtime U.S. Stem Cell shareholder and supporter, Knutson brings more than 30 years of business and financial experience to the organization. During his entrepreneurial career, Knutson founded Concrete Specialists, Inc. and continues to serve as its President; is the founder and current President of Sunwood Properties; and is the founder and Managing Partner of G&G Land Development, LLC.

The release continues to state matters more plainly: Demonstrating a progressive step forward, Knutson joins the Board of Directors just as the two companies amicably settled a legal dispute related to NorthStars preferred shares.

Weve witnessed in excess of 550% piled on for shareholders of the listing during the trailing month, a bounce that has taken root amid largely bearish action over the larger time frame. The situation may be worth watching. USRM has a history of dramatic rallies. Moreover, the listing has benefitted from a jump in recent trading volume to the tune of approaching 500% over the long run average.

US Stem Cell has a chunk ($246K) of cash on the books. But that total must be weighed against a mountain of about $3.3M in total current liabilities. One should also note that debt has been steadily growing over recent quarters. USRM is pulling in trailing 12-month revenues of $2.7M. In addition, the company is seeing major top line growth, with y/y quarterly revenues growing at 30.9%. Given the volume and volatility now involved, this may be a very interesting story and we will look forward to updating it again soon. For continuing coverage on shares of $USRM stock, as well as our other hot stock picks, sign up for our free newsletter today and get our next hot stock pick!

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More Clarity Emerges on US Stem Cell Inc (OTCMKTS:USRM) - The Oracle Dispatch

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New Cardiac Stem Cell Therapy passes Phase I/II Trials – Labiotech.eu (blog)

Posted: March 13, 2017 at 8:42 pm

TiGenix announces positiveone-year results forits phase I/II trial of donor-derived cardiac stem cell therapy in acute myocardial infarction (AMI).

The Belgian biotech TiGenixis developing allogeneic stem cell therapies. Now the companyhasannouncedthat its cardiac stem cell therapyAlloCSC-01 reached its primary endpoints in aphase I/IItrial.

In 2015, the companyacquired Coretherapixin a292M deal for its allogeneic cardiac stem cell pipeline, which is being developed for the treatment of AMI.The first-in-human trial was designed to test the safety and feasibility of an intracoronary infusion of donor-derivedexpanded cardiac stem cells (AlloCSCs)in patients with AMI and left ventricular dysfunction.

AlloCSC-01consists of adult allogeneic cardiac stem cells isolated from the heartof donors and expanded in vitro. In vivo studies suggest that these cellshave cardio-reparative potential by activating regenerative pathways and promoting the formation of new hearttissue.

Thecurrent phase II study demonstrated thesafety of these allogeneic stem cells. Initial results also revealed a larger reduction of infarct size in a subgroup of patients.

Myocardial infarction caused by blockade of coronary arteries

TiGenix is well known forChondroCellect, which was the first cell therapyto reach the European market for the repair of knee cartilage.After the companyrecently withdrew its market authorization for this product, due to a lack of reimbursement, the biotech is focusing on another stem cell therapy, Cx601, in addition to AlloCSC-01. Under development for Crohns disease, Cx601 is currently awaitingEMA approval and is in phase III trials in the US.

For a late-stage clinical company, TiGenix has a low market cap of191M. Even so, the company seems to be doing well these days with the progress of Cx601 and AlloCSC-01.

If AlloCSC-01 obtains market approval, it could treat the more than 1.9 millionpeople affected by AMI, a major cause of heart failure. So far, most treatments are palliative or restore myocardial function by angioplasty and insertion of a stent to support the vascular lumen.

Stem cell therapy of the heart is definitely not a new topic, but many trials have been conducted using the patients own stem cells derived from the bone marrow. A recent meta-analysisof such trials has suggested that these therapies are safe, but do not enhance cardiac function. TiGenixs approach using allogeneic heart-derived stem cells may offer a new and promisingopportunity in thefield.

Images via shutterstock.com / Liya Graphics andVeronika Zakharova

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New Cardiac Stem Cell Therapy passes Phase I/II Trials - Labiotech.eu (blog)

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Fate Therapeutics cleared to begin clinical trial assessing NK cell therapy in blood cancer; shares up 9% – Seeking Alpha

Posted: March 13, 2017 at 8:42 pm

Thinly traded micro cap Fate Therapeutics (FATE +9.4%) jumps out the blocks on more than a 6x surge in volume in response to its announcement that the FDA has approved its Investigational New Drug (IND) application for FATE-NK100, an adaptive memory natural killer (NK) cell product. The approval paves the way for a first-in-human clinical trial in patients with advanced acute myeloid leukemia (AML).

The early-stage study, to be conducted at the University of Minnesota, will determine the maximum tolerated dose of a single intravenous administration of FATE-NK100 in an accelerated dose escalation design in four patients.

FATE-NK100 is a next-generation immunotherapy based on a subset of NK cells that have shown robust anti-tumor activity in addition to promoting endogenous T cell response while resisting immune checkpoints.

NK cells identify and kill cancer cells by recognizing a range of stress signals expressed on tumor cells. Normal healthy cells are unaffected/unharmed. This is a different mechanism of action compared to T cells which require a specific tumor antigen to elicit an immune response and must be customized for each patient. The company says a significant advantage of NK-based immunotherapy is the avoidance of graft-versus-host disease, a common and potentially serious side-effect of T cell therapies.

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Fate Therapeutics cleared to begin clinical trial assessing NK cell therapy in blood cancer; shares up 9% - Seeking Alpha

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Gluten-free diets may be tied to an increased risk of Type 2 diabetes – Washington Post

Posted: March 13, 2017 at 8:41 pm

March 13 at 10:00 AM

Gluten-free diets are all the rage, but shunning gluten may offer no benefit to overall health for most people, a new analysis suggests.

In fact, the people in the study who ate more gluten were 13 percent less likely to develop Type 2 diabetes over the 30-year study than those who ate less gluten, the researchers found.

For some individuals, there are health reasons to avoid gluten, a protein found in grains such as wheat, rye and barley. Certain people, for example, have an intolerance to gluten, which can lead to abdominal pain, bloating or fatigue. Others have celiac disease, an autoimmune disorder that affects mostly the small intestine; when people with this disease eat gluten, their immune system responds by attacking the intestines lining.

However, even some people who do not have celiac disease or an intolerance to gluten believe that gluten-free diets are healthier than those that include gluten products, and the researchers wanted to see whether this belief might have any scientific merit, said lead study author Geng Zong, a nutrition research fellow at Harvard Universitys T.H. Chan School of Public Health in Boston.

[Science you can eat]

In the study, the researchers looked at surveys conducted every two to four years in which nearly 200,000 people reported what they ate. The researchers estimated the participants gluten intake based on this information, and then looked at which participants went on to develop Type 2 diabetes over the 30-year study period. Type 2 the most common form of diabetes occurs when the body has lost the ability to use insulin efficiently. This inability leads to high blood sugar levels, which can damage blood vessel walls, nerves and other tissues.

The researchers focused on studying the participants risk of diabetes because this condition is one of the leading causes of death in the United States, Zong said.

By the end of the study, nearly 16,000 particIpants had developed Type 2 diabetes. The researchers found that those who ate the most gluten had a 13 percent lower risk of developing Type 2 diabetes during the study period than the people who ate the least gluten.

These findings suggest that there might be a link between gluten consumption and risk of diabetes, the researchers said. However, it is not clear why the people who ate more gluten were less likely to be diagnosed with Type 2 diabetes than those who ate less gluten, the researchers said.

One possible explanation is that the people who consumed more gluten also ate more fiber, which, as previous research has suggested, may help to lower a persons diabetes risk. However, more research is needed to examine the relationship between gluten consumption and diabetes, the researchers said.

Live Science

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Gluten-free diets may be tied to an increased risk of Type 2 diabetes - Washington Post

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Nutrition label confusion poses problems for those with diabetes – Uniontown Herald Standard

Posted: March 13, 2017 at 8:41 pm

Diabetes can pose a number of challenges to those with the diagnosis.

With a heavy emphasis on foods, content and the impacts of those choices, understanding nutrition labels can be a daunting task.

Theres a lot of label confusion, so were here to help, said Patricia Zimmerman, a registered dietitian and a certified diabetes educator with the Diabetes Support Group through Monongahela Valley Hospital.

Tomorrow, the group is hosting a Health Eating Supermarket Tour at the Willow Pointe Shop & Save in Belle Vernon.

Learn how to choose foods at the supermarket to manage a healthier lifestyle, the program reads. Health care professionals from Monongahela Valley Hospitals Center for Diabetes and Endocrinology will conduct the tours and discuss how to live a healthier lifestyle.

Zimmerman said a diabetes educator will accompany the group through aisles as they highlight and analyze different carbohydrates, fiber sources and sugar content. Those in attendance also learn about meal planning and shopping on a budget.

The free program requires advanced registration, Zimmerman said, adding that people can call 724-258-1483 for more information.

Well do whatever we can to improve food choices and selections, she said. And our biggest highlight is making sure theyre comfortable with reading a nutrition label.

A lot of people have questions about better choices, said Zimmerman. We also have questions from people about whether they need supplements.

Zimmerman said a common misconception is associated with sugar-free items.

Sugar-free doesnt mean lower carbs. They forget theres other forms of carbs, she said. We want to steer them in the right direction.

Heidi McClain, a registered dietician and certified diabetes educator with Highlands Hospital Diabetes Center, agreed. McClain, who is the only registered dietician in Fayette County that deals with outpatient care, added that she advises diabetics to steer clear of sweetened, sugary drinks.

Free up the limited number of calories you have save them for eating. There are so many calorie-free drinks to chose from, McClain said. She also suggested adding more whole grains and foods with higher fiber content.

As for trips to the supermarket, McClain said one of the keys to success is going with, and sticking to, a shopping list.

Dont shop when youre hungry, McClain said. And stick to the perimeter of the store. Theres where the dairy, meats, fruits and veggies are located.

If you have to go into the inner aisles, get what you need and get out, she added with a laugh. Itll also cut back on impulse buying.

McClain also stressed the importance of understanding labels, noting that basic label reading can help people eat less, lose weight and focus on the foods contents to help blood sugar controls.

Most of us eat on autopilot. We eat because its there in front of us, whether we need it or not, she said. Label reading will make you more aware. Its the first step.

McClain added that there are several classes dealing with nutrition labels and food in general at the diabetes center. More information can be found by calling 724-628-8008.

The American Diabetes Association has a number of resources for those interested in learning more about what they can and cant eat.

In particular, there is information on diabetes superfoods that are chalk-full of vitamins, minerals and fibers, information on grains, starchy and non-starchy vegetables, and on what to eat, or drink, when going out.

They go as far as to provide a sample shopping list with refrigerated items including yogurt, skim milk, cottage cheese, fresh meat or poultry, and fruit/vegetables.

Pantry items include canned vegetables, rice, tuna, pasta and nuts.

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Tennova brings awareness to Diabetes Alert Day | WATE 6 On Your … – WATE 6 On Your Side

Posted: March 13, 2017 at 8:41 pm

KNOXVILLE (WATE) The frequency of people being diagnosed with type 2 diabetes has tripled in the past 20 years in the United States, according to Tennova Healthcare. March 28 is Diabetes Alert Day.

Diabetes affects nearly 30 million people in the United Statesa stunning 10 percent of the overall population, said Cheri Johnston, M.D., a family medicine physician with Tennova Primary Care Farragut. And recent research reveals that diabetes is now the third leading cause of death and not the seventh, as was previously thought. Perhaps the most alarming statistic is that one in four people living with diabetes is unaware that he or she even has the disease.

Tennova says the prevalence is due to an increase in sugary diets and sedentary lifestyles. One-third of Americans will have diabetes by 2050, according to researchers.

The disease can cause kidney failure, blindness, limb amputations and death if left untreated.

People with a family history of type 2 diabetes are at risk. Also, above-average body weight can lead to an increase. The disease is more common in Native Americans, African-Americans, Hispanics and Asian Americans.

On the positive side, diabetes is almost always avoidableeven reversiblewith appropriate lifestyle changes, Dr. Johnston said. Early diagnosis and treatment are critical to preventing significant damage to your health and longevity. Awareness and access to quality medical care are key.

Tennova suggests five ways to help reduce the risk of getting the disease:

As part of your annual physical, talk to your doctor about the results of your fasting blood sugar test and A1C test, which provides information about your average glucose levels over a three-month period, Dr. Johnston said. If your numbers are heading in the wrong direction, acting quickly may get things back on the right track.

For more information, call 1-855-TENNOVA or visit Tennovas website.

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Biological Industries USA Signs Agreement with Mill Creek Life … – Business Wire (press release)

Posted: March 12, 2017 at 6:46 pm

CROMWELL, Conn.--(BUSINESS WIRE)--Biological Industries USA (BI-USA), a division of Biological Industries Israel LTD, announced today a distribution agreement with Mill Creek Life Sciences LLC, a Rochester, Minnesota start-up that provides tools to support the development and application of cellular and biological therapeutics. The agreement provides for distribution of PLTMax Human Platelet Lysate, a product derived from human platelets and offers unparalleled cell growth and cytogenetic stability of adult stem cells over fetal bovine serum (FBS). PLTMax is available in research and clinical grades and has been used in more than 30 clinical trials worldwide, including several Phase III trials.

We are very pleased to announce our partnership with the team at Mill Creek Life Sciences, said Tanya Potcova, Chief Executive Officer of BI-USA. We have a great opportunity to expand our stem cell portfolio reach here in the US and beyond. We met with Mill Creek at the Cell & Gene Meeting on the Mesa in San Diego last October, where we discussed the potential use of PLTMax with our xeno-free NutriStem MSC Medium and EndoGo XF Medium. We found that we have a mutual interest in serving the needs of the stem cell industry through application development by pairing our leading technology together for use in both research and clinical development. We are excited about the potential for us to expand this collaboration.

Mill Creek Life Sciences is happy to announce this relationship withBiological Industries, said Bill Mirsch, Chief Executive Officer of Mill Creek Life Sciences. We tested their NutriStem MSC Medium and EndoGo XF Medium and found them to be very effective when paired with our PLTMax. We look forward to partnering with BI-USA to provide the best research and GMP grade media options for MSC and endothelial cell therapies.

About Biological Industries

Biological Industries (www.bioind.com) is one of the worlds leading and trusted suppliers to the life sciences industry, with over 35 years experience in cell culture media development and GMP manufacturing. BIs products range from classical cell culture media to supplements and reagents for stem cell research and potential cell therapy applications, to serum-free media and many other products for animal cell culture and molecular biology. BI is committed to a Culture of Excellence through advanced manufacturing and quality-control systems, regulatory expertise, in-depth market knowledge, and extensive technical customer-support, training, and R&D capabilities.

Biological Industries USA (BI-USA) is the US commercialization arm of BI, with facilities in Cromwell, Connecticut. Members of the BI-USA team share a history and expertise of innovation and success in the development of leading-edge technologies in stem cell research, cellular reprogramming, and regenerative medicine.

To receive ongoing BI communications, please join ouremail listor connect with the company onLinkedIn,Twitter, andFacebook.

About Mill Creek Life Sciences

Mill Creek Life Sciences (www.millcreekls.com) is the first company to commercialize human platelet lysate for cellular therapy. A spin-off out of the Human Cell Therapy Laboratory at the Mayo Clinic in Rochester, Minnesota, Mill Creek has been involved in clinical cellular therapies from the beginning. Mill Creek is dedicated to providing the highest quality products for the research and clinical community.

To find out more about Mill Creek Life Sciences, pleaseContact Usor connect with the company onLinkedIn,Twitter, andFacebook.

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