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Moffitt researchers discover mechanism leading to drug resistance, metastasis in melanoma

Posted: January 13, 2015 at 9:54 am

EphA2 protein activity increases aggressive behavior of melanoma cells treated with B-Raf inhibitor drugs

H. Lee Moffitt Cancer Center & Research Institute

Moffitt Cancer Center researchers have discovered a mechanism that leads to resistance to targeted therapy in melanoma patients and are investigating strategies to counteract it. Targeted biological therapy can reduce toxicity and improve outcomes for many cancer patients, when compared to the adverse effects of standard chemotherapeutic drugs. However, patients often develop resistance to these targeted therapies, resulting in more aggressive cells that can spread to other sites or cause regrowth of primary tumors.

B-Raf is a protein that is frequently mutated in human cancers, leading to increased tumor cell growth, survival and migration. Drugs that target B-Raf or another protein in the same network called MEK have proved effective in clinical trials. Several B-Raf and MEK inhibitors have been approved with the combination of a B-Raf and a MEK inhibitor being the current standard of care for patients with B-Raf mutant melanoma. However over time many patients become resistant to B-Raf and B-Raf/MEK inhibitor therapy.

Moffitt researchers found that patients who are on B-Raf inhibitor drugs develop more new metastases than patients who are on standard chemotherapy. The researchers wanted to determine how this acquired resistance develops in order to devise better treatment options for patients. They found that melanoma cells that are resistant to B-Raf inhibitors tend to be more aggressive and invasive, thereby allowing the tumor to spread to a new organ site. They used a large screening approach and discovered that this resistance and aggressive behavior was due to high activity of a cell surface protein called EphA2, which is also found on glioblastoma stem cells.

Their study found that simply withdrawing the B-Raf or MEK inhibitor drugs reversed the cells' aggressive behavior. "This suggests that alternate dose scheduling where B-Raf and MEK inhibitors are given to patients intermittently may reduce the aggressiveness of the disease... meaning patients could stay on therapy for more time," said Keiran S. Smalley, Ph.D., scientific director of the Donald A. Adam Comprehensive Melanoma Research Center of Excellence at Moffitt.

The research also showed that targeting EphA2 reduced the aggressive behavior of the melanoma cells. This suggests that drugs that target EphA2 may prevent the development of new disease in patients who receive B-Raf and B-Raf /MEK inhibitor therapy.

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The study was published in the online edition of Cancer Discovery on Dec. 26. It was funded by grants from the National Institutes of Health (R01 CA161107-01 and P50 CA168536-01A1), Melanoma and Sarcoma Groningen Foundation and the Joanna M. Nicolay Melanoma Foundation.

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The Future of Stem Cells: Opportunities at the Cutting Edge of Science

Posted: January 12, 2015 at 11:47 am

Stem cell technology representsone of the most fascinating and controversial medical advances of the past several decades. By now the enormous controversy which surrounded the use of federal funds to conduct scientific research on human stem cells during the George W. Bush administration has largely blown over. Five years have passed since President Obama lifted federal funding restrictions, and amazing progress has already been made in the field.

One can make a good case for stem cells being the most fascinating and versatile cells in the human body. This is precisely due to their stem role. In their most basic form, theyre capable of both replicating themselves an unlimited number of times and differentiating themselvesinto a huge number of other cell types. Muscle cells, brain cells, organ cells, and many others can all be created from stem cells. If youre interested, the NIH has an awesome introductionon stem cells on their website.

The question which has arisen since the discovery of thisamazing cell type has been how to harness their power and versatility. This is the primary focus of research today: how can we precisely control stem cells to perform whatever tasks we need them to do? Of course, other important issues, such as figuring out thebest places from which to harvest stem cells,exist.

Because of their role in the body, the number of potential applications for stem cells are truly stunning. From building custom cell clusters with 3D printers to curing a variety of diseases through bone marrow transplants, growingorgans for transplants, andeven growing edible meat, research is progressing at a frantic pace.

There are two particular areas of research which seem to hold the greatest promise at this point. The first is organs. Anyone who has ever been involved in an organ transplant knows how incredibly complex and difficult the process is. But difficulties like finding the right donor, preserving the organ, and finding enough supply to meet the incredible demand could all be overcome if we could simply use stem cells to grow a custom organ for each transplant from scratch.

Besides this perhaps science-fiction-sounding process of growing organs, theres also incredible excitement surrounding the potential of bone marrow transplants to cure diseases like HIVand Leukemia. This is done by implanting stem cells containing genetic mutations which confer immunity to a variety of diseases into a patients bone marrow, where they can begin naturally replicating and affecting the immune system.

Thisprocedurealso covers transplants designed simply to reintroduce healthy stem cells to help tackle a wider variety of ailments. Often, referred to as regenerative medicine as itinvolves stimulating the bodys preexisting repair mechanisms to help the healing process,thisprocedurealso offer great promise.

Naturally, the speed at which advances are being made in the field has led to problems as well. One recent well-publicized study which seemed to point to the possibility of achieving stimulus-triggered acquisition of pluripotency (essentially demonstrating a new type of stem cells) is now believedto have beenfraudulent.

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Renowned professor's book addresses stem cell biology & regenerative medicine

Posted: January 12, 2015 at 11:41 am

IMAGE:This is the cover for Stem Cells, Tissue Engineering and Regenerative Medicine. view more

Credit: World Scientific, 2015

In his latest book published by World Scientific, Professor David Warburton from The Saban Research Institute of Children's Hospital Los Angeles and the University of Southern California presents a collection of essays on the current state of the regenerative medicine and stem cell research field.

Entitled Stem Cells, Tissue Engineering and Regenerative Medicine, this up-to-date compendium surveys current issues in stem cell biology and regenerative medicine. Topics range from key concepts in regenerative medicine to the newest progenitor cell therapies for organ systems, to advice on how to set up a pluripotent stem cell laboratory.

Overviews of the most recent progress in stem cell research describe work that is in the pre-clinical pipeline from scientists working at The Saban Research Institute of Children's Hospital Los Angeles and colleagues around the world.

"The book addresses some of the big questions faced by researchers in the field of stem cell biology and regenerative medicine," said Professor Warburton. "Those of us working in this field in California are positively impacted by the critical funding provided by the citizens of the state through the California Institute for Regenerative Medicine. I believe this book shows that the hope behind CIRM - the hope that stem cells can really revolutionize medicine and human health - is fully justified."

A global collection of essays from collaborating investigators in Australia, Brazil, Iran, Taiwan and the United Kingdom, as well as across the United States. This book will describe diverse regenerative medicine solutions for airways, cancer, craniofacial structures, intestine, heart, kidney, liver, lung and nervous system. These advances are placed in the context of the overall field, providing an investigator-level overview which will be accessible to the educated scientific generalist as well as a college-educated readership, scientific writers, educators and professionals of all kinds.

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Professor Warburton's research is supported by the California Institute for Regenerative Medicine, the National Institutes of Health: National Heart, Lung and Blood Institute, National Institute of Environmental Health Sciences, Fogarty International Center, National Institute of General Medical Sciences, The Pasadena Guild of Children's Hospital Los Angeles, The Santa Anita Foundation, The Webb Foundation, The Garland Foundation and anonymous venture philanthropy.

The book retails for US$155/ 102 (hardcover). More information on the book can be found at http://www.worldscientific.com/worldscibooks/10.1142/9212.

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Renowned professor's book addresses stem cell biology & regenerative medicine

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Iran cloning animals on large scale in Institute of Stem Cell Research, Royan – Video

Posted: January 11, 2015 at 10:43 pm


Iran cloning animals on large scale in Institute of Stem Cell Research, Royan
IRIB 1 news report on cloning animals on large scale in Institute of Stem Cell Research, Royan Royan Institute: http://www.royaninstitute.org/cmsen/index.php.

By: ali javid

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Iran cloning animals on large scale in Institute of Stem Cell Research, Royan - Video

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Novel cancer stem cells & glioma cell invasion – Dr Paul Brennan – Video

Posted: January 11, 2015 at 9:46 am


Novel cancer stem cells glioma cell invasion - Dr Paul Brennan
Dr Paul Brennan, University of Edinburgh, talks about his research into the identification of a novel cancer stem cell that might be important for glioma cel...

By: acmedsci

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Jeunesse Global Revolutionary Anti Aging Skin Care with Stem Cells – Video

Posted: January 11, 2015 at 9:46 am


Jeunesse Global Revolutionary Anti Aging Skin Care with Stem Cells

By: Karina Palmer

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Cancer Care Northwest Stem Cell Transplantation

Posted: January 10, 2015 at 3:55 pm

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Uses your own healthy stem cells to fight your cancer.

High-dose chemotherapy can be very effective in destroying certain cancers, but the intense therapy can also damage healthy cells and increase your chance of infection and other health problems.

In a process called autologous stem cell transplantation, doctors collect your own stem cells before you receive the high-dose chemotherapy. They return the healthy stem cells to you after treatment, improving your bodys ability to recover.

Before your high-dose chemotherapy, your doctor uses a machine similar to a dialysis machine to collect and temporarily store your stem cells. Stem cells, found mainly in bone marrow, are the cells from which all blood cells develop.

After the high-dose chemotherapy is delivered to kill the cancer cells, your healthy stem cells are returned to you intravenously (IV) to replace the stem cells that were destroyed by the therapy. Your body uses these stem cells to reestablish your bone marrow where your blood cells are produced.

This is very different than the often controversial field of stem cell research where stem cells done on embryos.

Stem cell transplantation and high-dose chemotherapy is most often used to treat patients with multiple myeloma and recurrent lymphoma (including Hodgkin and non-Hodgkin lymphoma). This treatment modality is occasionally used to treat other cancers such as recurrent testicular cancer.

Cancer Care Northwest is a regional leader in stem cell transplantation.

Our very own Dr. Hakan Kaya is director of the Inland Northwest Myeloma/Lymphoma and Transplant Program, a collaboration between Cancer Care Northwest, Deaconess Medical Center and the Inland Northwest Blood Center.

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Cancer Care Northwest Stem Cell Transplantation

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"Latest Stem Cells News" – news from the world about stem …

Posted: January 10, 2015 at 3:54 pm

For years, researchers seeking new therapies for traumatic brain injury have been tantalized by the results of animal experiments with stem cells. In numerous studies, stem cell implantation has substantially improved brain function in experimental animals with brain trauma. But just how these improvements occur has remained a mystery.

Now, an important part of this puzzle has been pieced together by researchers at the University of Texas Medical Branch at Galveston. In experiments with both laboratory rats and an apparatus that enabled them to simulate the impact of trauma on human neurons, they identified key molecular mechanisms by which implanted Read More

For the first time, cloning technologies have been used to generate stem cells that are genetically matched to adult patients.

Fear not: No legitimate scientist is in the business of cloning humans. But cloned embryos can be used as a source for stem cells that match a patient and can produce any cell type in that person ()

This is a dream that weve had for 15 years or so in the stem cell field, said John Gearhart, director of the Institute for Regenerative Medicine at the University of Pennsylvania. Gearhart first proposed this approach for patient-specific stem cell generation in Read More

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DURHAM To donate the stem cell-rich umbilical cord blood produced during the birth of her daughter, Jaime Feaster of Lake Charles, La., would have had to drive more than two hours to the nearest hospital equipped to collect it.

Thats a long way to go when youre in labor.

Instead, Feaster turned to a fledgling Duke University Medical Center program that provides collection kits to mothers and their doctors. When Feasters daughter, Kadee, arrived last month, the cord blood was collected, packaged and quickly shipped to a blood bank at no cost to Feaster and with minimal commitment Read More

Baldness is an undesirable condition that afflicts both men and women, many of which have family members with significant hair loss. According to Health Day News, a new study of stem cells in mice shows promise for future treatments for people who battle hair loss.

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VICTORIES & SUCCESS STORIES | The Stem Cell Blog | Page 8

Posted: January 10, 2015 at 3:52 pm

Stem cell therapy goes to the dogs

A surgical team at Veterinary Specialty Center in Buffalo Grove removes fat from Doodle, a 9-year-old German Shepherd suffering from osteoarthritis and hip dysplasia. Stem cells will be derived from the fat and injected into the dog. Photo submitted by Veterinary Specialty Center

Doodle was the first dog to receive the new one-day stem cell procedure in Illinois./Photo submitted by Veterinary Specialty Center

Things were getting bad for Doodle. Despite her youthful name, the 9-year-old German Shepherd was experiencing joint pain from bilateral hip dysplasia and osteoarthritis. She would get sore and tired from long weekend walks and started falling up the stairs.

Her owners, the Dahl family of Oak Brook, had tried different options before landing on animal stem cell regenerative therapy, a procedure thats a hot topic in the veterinary world. Last week, Doodle received reportedly the first such one-day operation in Illinois at the Veterinary Specialty Center in Buffalo Grove.

The practice of using stem cells, derived from the animals fat, to treat joint problems could be discouraging for pet owners because of cost and timing. The animal used to have to go twice to a vet hospital: once for surgery to remove fat cells and once again for the injection of the stem cells into the inflamed joint. The cost was around $2,700.

Leslie Dahl, Doodles owner and a veterinarian herself, didnt want to go that route. She had tried anti-inflammatory medication, but Doodles stomach couldnt handle it. She tried collagen injections, but they didnt fully relieve Doodle of her pain. Plus, the animal already was difficult at the vets and she was concerned that Doodle would get too anxious between the visits.

So when the Veterinary Specialty Center started looking into a new procedure that allows the stem cells to be processed in the same facility on the same day for about $1,900, Dahl was intrigued.

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Frontiers in Stem Cells & Regeneration – Marine Biological …

Posted: January 10, 2015 at 3:52 pm

Course Date: September 27 October 3, 2015

Deadline: July 7, 2015 | Online Application Form

2014 Agenda (PDF)

Contact for more information: pdc@pdc.magee.edu

Directors: Jennifer Morgan, MBL; and Gerald P. Schatten, University of Pittsburgh

The Stem Cells and Regeneration Course (formerly known as FrHESC) is a dynamic, evolving laboratory and lecture course that includes the complete array of biological and medical perspectives from fundamental basic biology of stemness and mechanisms of regeneration through evaluation of pluripotent stem cells for therapeutic benefit.

The NIH sponsored course is designed for postdoctoral fellows, newly independent scientists, and established investigators seeking comprehensive and sophisticated training in research strategies and state-of-the-art cellular, molecular and genetic approaches for advancing human embryonic stem cell research.

The course consists of daily lectures from resident faculty and other invited speakers, discussions and informal seminars, laboratory exercises and demonstrations, and one-on-one tutorials.

The Stem Cells and Regeneration Course will exclusively use human embryonic stem cell lines on the NIH Human Embryonic Stem Cell Registry and being routinely cultured at the Pittsburgh Development Center.

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