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Long lasting blood vessels created in mice using human stem cells

Posted: July 16, 2013 at 12:49 pm

Washington, July 16 : Researchers at Massachusetts General Hospital (MGH) have used vascular precursor cells derived from human induced pluripotent stem cells (iPSCs) to generate, in mice, functional blood vessels that lasted as long as nine months.

In their report, the investigators describe using iPSCs - reprogrammed adult cells that have many of the characteristics of embryonic stem cells - from both healthy adults and from individuals with type 1 diabetes to generate blood vessels on the outer surface of the brain or under the skin of mice.

"The discovery of ways to bring mature cells back to a 'stem-like' state that can differentiate into many different types of tissue has brought enormous potential to the field of cell-based regenerative medicine, but the challenge of deriving functional cells from these iPSCs still remains," Rakesh Jain, PhD, director of the Steele Laboratory for Tumor Biology at MGH and co-senior author of the study, said.

"Our team has developed an efficient method to generate vascular precursor cells from human iPSCs and used them to create networks of engineered blood vessels in living mice," he said.

The ability to regenerate or repair blood vessels could make a crucial difference in the treatment of cardiovascular disease -- which continues to be the number one cause of death in the US -- and other conditions caused by blood vessel damage, such as the vascular complications of diabetes.

In addition, providing a vascular supply to newly-generated tissue remains one of the greatest barriers facing efforts to build solid organs through tissue engineering.

Several previous studies have generated from iPSCs the types of cells required to build blood vessels -- endothelial cells that line vessels and connective tissue cells that provide structural support -- but those cells could not form long-lasting vessels once introduced into animal models.

"The biggest challenge we faced during the early phase of this project was establishing a reliable protocol to generate endothelial cell lines that produced great quantities of precursor cells that could generate strong, durable blood vessels," co-senior author Dai Fukumura, MD, PhD, also of the Steele Lab, said.

The MGH team adapted a method originally used to derive endothelial cells from human embryonic stem cells (hESCs).

But while that method used a single protein marker to identify vascular progenitors, the researchers sorted out iPSC-derived cells that expressed not only that protein but also two other protein markers of vascular potential.

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Long lasting blood vessels created in mice using human stem cells

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Inner ear structure created from mouse stem cells

Posted: July 16, 2013 at 12:49 pm

Washington, July 16 (ANI): Embryonic stem cells of mice have been transformed into key structures of the inner ear in a breakthrough study.

The discovery provides new insights into the sensory organ's developmental process and sets the stage for laboratory models of disease, drug discovery and potential treatments for hearing loss and balance disorders.

A research team led by Eri Hashino, Ph.D., Ruth C. Holton Professor of Otolaryngology at Indiana University School of Medicine, reported that by using a three-dimensional cell culture method, they were able to coax stem cells to develop into inner-ear sensory epithelia-containing hair cells, supporting cells and neurons-that detect sound, head movements and gravity.

Dr. Hashino said that the team determined that the cells needed to be suspended as aggregates in a specialized culture medium, which provided an environment more like that found in the body during early development.

The team mimicked the early development process with a precisely timed use of several small molecules that prompted the stem cells to differentiate, from one stage to the next, into precursors of the inner ear.

Karl R. Koehler, B.A., the paper's first author and a graduate student in the medical neuroscience graduate program at the IU School of Medicine, said that the three-dimensional suspension also provided important mechanical cues, such as the tension from the pull of cells on each other.

Koehler said that the 3-D culture allows the cells to self-organize into complex tissues using mechanical cues that are found during embryonic development.

Hashino said that the team was surprised to see that once stem cells are guided to become inner-ear precursors and placed in 3-D culture, these cells behave as if they knew not only how to become different cell types in the inner ear, but also how to self-organize into a pattern remarkably similar to the native inner ear.

He said that the team's initial goal was to make inner-ear precursors in culture, but when we did testing we found thousands of hair cells in a culture dish.

Electrophysiology testing further proved that those hair cells generated from stem cells were functional, and were the type that sense gravity and motion.

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Inner ear structure created from mouse stem cells

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How stem cells increase libido and enhance looks

Posted: July 16, 2013 at 12:49 pm

Stem cell therapy results in dramatic decrease in blood sugar, improved sleep, increased energy levels and shoulder mobility, and improved libido. But StemGenics says patients must be tested first if theyre up to it By Marge C. Enriquez Philippine Daily Inquirer

OPERATION room maintains hospital standards.

Sam Fernandez (not his real name), a businessman in his late 50s, who underwent stem cell treatment, excitedly called his doctor, about its immediate effects. Although the benefits were supposed to be visible six to eight weeks after the procedure, the patient felt like a young man again in six days.

His doctor gave him intravenous therapy, directly injecting stem cell on the shoulder to address the chronic pains and the penis for erectile dysfunction. Aside from the dramatic decrease in blood sugar, improved sleep, increased energy levels and shoulder mobility, the patient reported increased libido. He claimed he was getting woody (slang for erection) every day that his wife couldnt keep up with him.

As the age management medicine trend, there are many hospitals and aesthetic centers offering stem cell treatment at the average price of P1.2 to P1.4 million. Accredited by the Department of Health, StemGenics not only offers medical technology that is unique to the clinic but also procedures at a reasonable price range of P500,000.

Repairing tissues

Dr. Eduardo Santos, president of StemGenics, said that stem cell therapy is just one of the three aims of a health program: There is repair, maintain and defend, he said.

He explained: Maintenance is a function of exercise, nutritional supplement and hormone support. Defense is a function of guarding the body against cancer and infection. You boost the immune system. The repair is the stem cell procedure, our anchor service. The stem cell regenerates the tissues. You reintegrate the organs which need to be repaired.

Stem cells used for repair are found in the bone marrow, blood, fat or adipose tissues and in other parts of the body. These cells are stimulated upon getting alerts on injury or inflammation. Their task is to precipitate the healing process when the body is injured. They send proteins to control the inflammation and refresh new cells, and engage new growth proteins. These cells are exchanging regenerative signals within the environment.

After birth, we have 80 to 100 million stem cells, said Santos. Over time, they decrease. By mid-30s, the count is down to 25 million. You wont have enough to heal as fast as you were younger. That is when aging happens. That is why disease associated with aging comes inheart conditions, dementia, decreased function of kidney and liver.

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BioTime CEO Dr. Michael West to Present at the Case Western Reserve University 6th Annual Regenerative Medicine …

Posted: July 16, 2013 at 12:49 pm

ALAMEDA, Calif.--(BUSINESS WIRE)--

BioTime, Inc. (NYSE MKT: BTX), a biotechnology company that develops and markets products in the field of regenerative medicine, announced that Chief Executive Officer Michael D. West, PhD will present today at the Case Western Reserve University in the meeting The Business of Regenerative Medicine: From Stem Cells to the Market Place in Cleveland, Ohio. Dr. Wests presentation titled Embryomics: Commercial Opportunities in the Increasing Complex Biology of Pluripotency is scheduled for 11:00 a.m. EDT. Dr. West will describe new data relating the company's PureStem technology and cell lines potentially useful in the treatment of Alzheimer's disease and for Alzheimer's disease research and will soon be made available for sale to the research community.

The presentation is available on BioTimes website at http://www.biotimeinc.com/scientific-presentations. A discussion of the results presented in todays meeting is also discussed by Dr. West in his weekly video update available for viewing at http://www.biotimeinc.com/video-series/.

About BioTime, Inc.

BioTime, headquartered in Alameda, California, is a biotechnology company focused on regenerative medicine and blood plasma volume expanders. Its broad platform of stem cell technologies is enhanced through subsidiaries focused on specific fields of application. BioTime develops and markets research products in the fields of stem cells and regenerative medicine, including a wide array of proprietary PureStem cell lines, HyStem hydrogels, culture media, and differentiation kits. BioTime is developing Renevia (formerly known as HyStem-Rx), a biocompatible, implantable hyaluronan and collagen-based matrix for cell delivery in human clinical applications. BioTime's therapeutic product development strategy is pursued through subsidiaries that focus on specific organ systems and related diseases for which there is a high unmet medical need. BioTime's majority owned subsidiary Cell Cure Neurosciences Ltd. is developing therapeutic products derived from stem cells for the treatment of retinal and neural degenerative diseases. BioTime's subsidiary OrthoCyte Corporation is developing therapeutic applications of stem cells to treat orthopedic diseases and injuries. Another subsidiary, OncoCyte Corporation, focuses on the diagnostic and therapeutic applications of stem cell technology in cancer, including the diagnostic product PanC-Dx currently being developed for the detection of cancer in blood samples. ReCyte Therapeutics, Inc. is developing applications of BioTime's proprietary induced pluripotent stem cell technology to reverse the developmental aging of human cells to treat cardiovascular and blood cell diseases. BioTime's subsidiary LifeMap Sciences, Inc. markets GeneCards, the leading human gene database, as part of an integrated database suite that also includes the LifeMap Discovery database of embryonic development, stem cell research and regenerative medicine, and MalaCards, the human disease database. LifeMap Sciences also markets BioTime research products and PanDaTox, an innovative, recently developed, searchable database that can aid in the discovery of new antibiotics and biotechnologically beneficial products. Asterias Biotherapeutics, Inc. is a new subsidiary being used to acquire the stem cell assets of Geron Corporation, including patents and other intellectual property, biological materials, reagents and equipment for the development of new therapeutic products for regenerative medicine. BioTime's lead product, Hextend, is a blood plasma volume expander manufactured and distributed in the U.S. by Hospira, Inc. and in South Korea by CJ CheilJedang Corporation under exclusive licensing agreements. Additional information about BioTime can be found on the web at http://www.biotimeinc.com.

Forward-Looking Statements

Statements pertaining to future financial and/or operating results, future growth in research, technology, clinical development, and potential opportunities for BioTime and its subsidiaries, along with other statements about the future expectations, beliefs, goals, plans, or prospects expressed by management constitute forward-looking statements. Any statements that are not historical fact (including, but not limited to statements that contain words such as "will," "believes," "plans," "anticipates," "expects," "estimates") should also be considered to be forward-looking statements. Forward-looking statements involve risks and uncertainties, including, without limitation, risks inherent in the development and/or commercialization of potential products, uncertainty in the results of clinical trials or regulatory approvals, need and ability to obtain future capital, and maintenance of intellectual property rights. Actual results may differ materially from the results anticipated in these forward-looking statements and as such should be evaluated together with the many uncertainties that affect the business of BioTime and its subsidiaries, particularly those mentioned in the cautionary statements found in BioTime's Securities and Exchange Commission filings. BioTime disclaims any intent or obligation to update these forward-looking statements.

To receive ongoing BioTime corporate communications, please click on the following link to join our email alert list: http://news.biotimeinc.com

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The Alliance for Regenerative Medicine Holds Third Annual Stem Cell Meeting on the Mesa Regen Med Partnering Forum

Posted: July 16, 2013 at 12:49 pm

WASHINGTON, DC--(Marketwired - Jul 16, 2013) - The Alliance for Regenerative Medicine (ARM), the international organization representing the interests of the regenerative medicine community, today announced that the third annual Regen Med Partnering Forum will take place October 14 and 15, 2013 as part of the Stem Cell Meeting on the Mesa. This forum is the only partnering meeting organized specifically for the regenerative medicine field, and has become the premier gathering place for more than 300 senior level executives seeking opportunities to collaborate and learn more about recent advances in the space. Life Technologies Corporation (NASDAQ: LIFE) is the meeting's founding and platinum-level sponsor.

The Regen Med Partnering Forum will take place at the Estancia La Jolla Hotel & Spa, La Jolla, California. The Stem Cell Meeting on the Mesa's eighth Annual Scientific Symposium will immediately follow the Regen Med Partnering Forum on October 16, 2013 at the Salk Institute for Biological Studies. The Scientific Symposium, organized by the Sanford Consortium for Regenerative Medicine, is attended by leading scientists and researchers in the field, as well as participants from the business and patient advocacy communities. Combined, these meetings will attract over 800 attendees from around the globe, highlighting the promise and progress of this rapidly evolving, interdisciplinary field.

"Stem Cell Meeting on the Mesa is the premier annual meeting for anyone involved in regenerative medicine, cell therapy and stem cell research," said Geoff MacKay, President & Chief Executive Officer of Organogenesis Inc. and Chairman of ARM. "The quality of speakers -- both industry and academic -- combined with high-level partnering opportunities, makes attending this meeting every year a top priority."

In addition to presentations by 40 cutting-edge companies seeking partners and investors, the Regen Med Partnering Forum will include interactive panels addressing critical commercial, scientific, regulatory and reimbursement issues. One-on-one meetings will also be scheduled with a state-of-the-art partnering system to connect participants. The Regen Med Partnering Forum was developed by the Alliance for Regenerative Medicine (ARM) and the California Institute for Regenerative Medicine (CIRM) in 2011 and has grown 35% over the past three years.

"Life Technologies has sponsored this meeting since its inception in 2006, and helped it grow into a leading, annual conference for the regenerative medicine community," said Alaine Maxwell, Associate Director, Americas Marketing, Stem Cells and Translational Research, Life Technologies. "Whether your goal is to gain exposure to researchers or put your name out in front of C-level executives, attending this meeting has incredible value."

To learn more or to register for the 2013 Stem Cell Meeting on the Mesa please visit http://www.stemcellmeetingonthemesa.com. Registration is complimentary for investors and credentialed members of the media.

About The Alliance for Regenerative MedicineThe Alliance for Regenerative Medicine (ARM) is a Washington, DC-based multi-stakeholder advocacy organization that promotes legislative, regulatory and reimbursement initiatives necessary to facilitate access to life-giving advances in regenerative medicine. ARM also works to increase public understanding of the field and its potential to transform human healthcare, providing business development and investor outreach services to support the growth of its member companies and research organizations. Prior to the formation of ARM in 2009, there was no advocacy organization operating in Washington, DC to specifically represent the interests of the companies, research institutions, investors and patient groups that comprise the entire regenerative medicine community. Today ARM has more than 140 members and is the leading global advocacy organization in this field. To learn more about ARM or to become a member, visit http://www.alliancerm.org.

About Life Technologies Life Technologies Corporation (NASDAQ: LIFE) is a global biotechnology company that is committed to providing the most innovative products and services to leading customers in the fields of scientific research, genetic analysis and applied sciences. With a presence in more than 180 countries, the company's portfolio of 50,000 end-to-end solutions is secured by more than 5,000 patents and licenses that span the entire biological spectrum -- scientific exploration, molecular diagnostics, 21st century forensics, regenerative medicine and agricultural research. Life Technologies has approximately 10,000 employees and had sales of $3.8 billion in 2012.

Life Technologies' Safe Harbor Statement This press release includes forward-looking statements about our anticipated results that involve risks and uncertainties. Some of the information contained in this press release, including, but not limited to, statements as to industry trends and Life Technologies' plans, objectives, expectations and strategy for its business, contains forward-looking statements that are subject to risks and uncertainties that could cause actual results or events to differ materially from those expressed or implied by such forward-looking statements. Any statements that are not statements of historical fact are forward-looking statements. When used, the words "believe," "plan," "intend," "anticipate," "target," "estimate," "expect" and the like, and/or future tense or conditional constructions ("will," "may," "could," "should," etc.), or similar expressions, identify certain of these forward-looking statements. Important factors which could cause actual results to differ materially from those in the forward-looking statements are detailed in filings made by Life Technologies with the Securities and Exchange Commission. Life Technologies undertakes no obligation to update or revise any such forward-looking statements to reflect subsequent events or circumstances.

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The Alliance for Regenerative Medicine Holds Third Annual Stem Cell Meeting on the Mesa Regen Med Partnering Forum

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Stem Cell Therapy Treatment for Idiopathic Polymyositis by Dr Alok Sharma, Mumbai, India. – Video

Posted: July 16, 2013 at 12:46 pm


Stem Cell Therapy Treatment for Idiopathic Polymyositis by Dr Alok Sharma, Mumbai, India.
Improvement seen in just 5 day after Stem Cell Therapy Treatment for Idiopathic Polymyositis by Dr Alok Sharma, Mumbai, India. After Stem Cell Therapy 1. Fle...

By: Neurogen Brain and Spine Institute

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Stem Cell Therapy Treatment for Idiopathic Polymyositis by Dr Alok Sharma, Mumbai, India. - Video

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Stem Cell Therapy Treatment for Left Hemiplegic Cerebral Palsy by Dr Alok Sharma, Mumbai, India. – Video

Posted: July 16, 2013 at 12:46 pm


Stem Cell Therapy Treatment for Left Hemiplegic Cerebral Palsy by Dr Alok Sharma, Mumbai, India.
Improvement seen in just 5 day after Stem Cell Therapy Treatment for Left Hemiplegic Cerebral Palsy by Dr Alok Sharma, Mumbai, India. After Stem Cell Therapy...

By: Neurogen Brain and Spine Institute

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Stem Cell Therapy Treatment for Left Hemiplegic Cerebral Palsy by Dr Alok Sharma, Mumbai, India. - Video

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Stem Cell Therapy Treatment for Autism by Dr Alok Sharma, Mumbai, India. Part 2 – Video

Posted: July 16, 2013 at 12:46 pm


Stem Cell Therapy Treatment for Autism by Dr Alok Sharma, Mumbai, India. Part 2
Improvement seen in just 7 months after Stem Cell Therapy Treatment for Autism by Dr Alok Sharma, Mumbai, India. After Stem Cell Therapy 1. Emotional respons...

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Stem Cell Therapy Treatment for Cerebral Atrophy by Dr Alok Sharma, Mumbai, India. Part 2 – Video

Posted: July 16, 2013 at 12:46 pm


Stem Cell Therapy Treatment for Cerebral Atrophy by Dr Alok Sharma, Mumbai, India. Part 2
Improvement seen in just 5 day after Stem Cell Therapy Treatment for Cerebral Atrophy by Dr Alok Sharma, Mumbai, India. After Stem Cell Therapy 1. Ball throw...

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ANN ARBOR: University of Michigan breast cancer clinical trial looks at targeting cancer stem cells

Posted: July 15, 2013 at 8:52 am

ANN ARBOR A major reason that breast cancer returns after treatment and spreads to other parts of the body is that current chemotherapy and radiation treatments do not kill the cancer stem cells.

That premise provides the basis for a clinical trial open at the University of Michigan Comprehensive Cancer Center and two other sites testing a drug that has been found in laboratory studies to attack cancer stem cells. The drug, reparixin, will be used in combination with standard chemotherapy.

This is one of only a few trials testing stem cell directed therapies in combination with chemotherapy in breast cancer, says principal investigator Dr. Anne Schott, associate professor of internal medicine at the University of Michigan. Combining chemotherapy with stem cell therapy has the potential to lengthen remissions for women with advanced breast cancer.

Cancer stem cells are the small number of cells within a tumor that fuel its growth and spread.

The phase Ib study will test reparixin, which is taken orally, along with the chemotherapy drug paclitaxel in women with HER2-negative metastatic breast cancer. The study is primarily intended to test how patients tolerate this drug combination, but researchers will also look at how reparixin appears to be impacting markers for cancer stem cells and signs of inflammation. The study will also look at how effective this treatment combination is at controlling the cancer and impacting survival.

The clinical trial stems from laboratory research at U-M that identified a receptor called CXCR1 on the cancer stem cells that triggers growth of stem cells in response to inflammation and tissue damage. Adding reparixin to chemotherapy in laboratory studies specifically targeted and killed breast cancer stem cells by blocking CXCR1.

Mice treated with reparixin or the combination of reparixin and chemotherapy had dramatically fewer cancer stem cells than those treated with chemotherapy alone. In addition, reparixin-treated mice developed significantly fewer metastases than mice treated with chemotherapy alone.

FYI

According to the American Cancer Society, 234,580 Americans will be diagnosed with breast cancer this year and 40,030 will die from the disease.

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