Freeze thaw chambers are also called refrigerated humidity chambers. Freeze thaw chamber is used for applications which require temperature cycling down below freezing. Principle of freeze thaw cycle is used in cryopreservation technique. Cryopreservation is the process of preserving living cells and tissues at cryogenic temperature, which lead to suspended metabolic activity of cells in liquid nitrogen. Principle of freeze thaw cycle is nowadays used as treatment method for cancer, as freezing temperature is used in cryosurgery for local tissue destruction. Freezing and thawing cause cell death due to ice crystal formation, osmotic shock, and membrane damage. Advanced freeze thaw chambers are available in the market with new features, such as, epoxy coating to avoid corrosion, better condenser and evaporator, capillary tube system, CFC-free polyurethane foam for insulation, broad temperature range from -20 C to 60 C, and long service life designs.

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Rise in understanding about the physical and chemical properties of freeze and thaw cycle, advancement in the field of mechanics, and flexibility to stimulate a broad range of conditions are some of the factors driving the growth of the freeze thaw chambers market. However, expensive maintenance and stringent regulatory standards are anticipated to hamper the growth of the market during the forecast period. Customized freeze thaw chambers are the ones which can be modified based on the requirement of the end-user. Introduction of customized freeze thaw instruments is likely to boost the growth of the freeze thaw chambers market during the forecast period.

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On the basis of application area, the global freeze thaw chambers market can be segmented into biopharmaceutical development, molecular biology and biochemistry, medical application, and others. Medical application can be further divided into cryopreserved products and others. Cryopreserved products can be further classified into blood transfusion, bone marrow transplantation, artificial insemination, in vitro fertilization (IVF), stem cell and organ, and others. The biopharmaceutical development segment is propelling the growth of the freeze thaw chambers market as freezing is one of the processing steps in drug development that ensures the stability and quality of drugs, while freeze thaw chambers provide stability to drug substances. Freeze thaw chambers are also used for ASTM material testing. The cryopreserved products segment is expected to drive the growth of the freeze thaw chambers market during the forecast period due to increase in awareness about the importance and function of stem cell and tissue engineering. Advancement in organ transplantation technology is also another important factor contributing to the growth of stem cell and organ cryopreservation. Freeze thaw chambers are extensively used to transfer cryopreserved products to the end-user for applications, such as, blood transfusion, bone marrow transplantation, artificial insemination, and in vitro fertilization (IVF).

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Based on end-user, the global freeze thaw chambers market can be segmented into biopharmaceutical companies, pathology and research laboratories, hospitals, and stem cell and blood banks. The pathology and research laboratories segment is boosting the growth of the freeze thaw chambers market as these chambers are required for research applications which require very low freezing temperature. Biopharmaceutical companies is another growing segment of the freeze thaw chambers market. Freeze thaw chambers maximize productivity and reduce production cost by providing flexibility to drug substances.

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In terms of region, the global freeze thaw chambers market can be categorized into North America, Europe, Asia Pacific, Lain America, and Middle East & Africa. North America is the leading market for freeze thaw chambers in the world due to extensive funding support from the government for research activities in the region. Europe is another leading market for freeze thaw chambers market owing to extensive research in the field of biochemistry and molecular biology in the region. The freeze thaw chambers market in Asia Pacific is expected to grow significantly during the forecast period due to rise in awareness in the region regarding medical applications involving cryopreservation process, such as, umbilical cord, stem cell, and blood sample.

Key players operating in the global freeze thaw chambers market are Darwin Chambers, Newtronic Lifecare Equipments Pvt. Ltd., Caron Products and Services, Inc, BIONICS SCIENTIFIC TECHNOLOGIES (P) LTD., Santorius, Feutron Klimasimulation GmBH., LR Environmental Equipment Co.Inc., and Dycometal.

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Knee Implant Market:

The steadily increasing occurrence of post-traumatic arthritis, rheumatoid arthritis, and osteoarthritis is likely to provide immense growth prospects for the global knee implant market. Furthermore, due to the poor satisfaction levels of patients getting total knee replacement, knee arthroplasty has presented an opportunity for major competitors in the industry. As a result, producers have begun to utilize different biomaterials for improved strength to meet this growing demand, which is likely to drive global knee implant market in the forthcoming years. Furthermore, due to rising success rates, technical breakthroughs, and the simple availability of durable and higher-quality implants, knee replacement treatments are in great demand.

Ophthalmic Lasers Market:

One of the key factors responsible for driving the growth of the global ophthalmic lasers market is the increasing number of patients suffering from numerous eye diseases across the world. There are study reports that show the rate of occurring partial or full blindness has considerably increased over the period and could still double to its current volume by 2020. This growth is mainly down to the diseases such as cataract, glaucoma, and diabetes. The increasing awareness about the benefits of timely diagnosis and productive medical care such as laser operation could avoid occurrence of these conditions is also helping to drive the growth of the global ophthalmic lasers market. Moreover, increasing awareness among people about the effectiveness of the treatment methods that are available is also projected to fuel the market growth in coming years.

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ST. LOUIS (AP) Jake Litvag leaned in for a closer look as a lab mouse scurried around an enclosure, stopping to sniff a large block.

Hi, Jakob 1. Im Jake, the 16-year-old said, naming the little furry creature engineered to have the same genetic abnormality he has.

That mouse and its lab-grown relatives are the first in the world to mirror the missing gene that causes Jakes autism. Scientists at Washington University in St. Louis bred the mice, and grew stem cells derived from Jakes blood, to study and find ways to treat his rare disorder and look for answers to the larger puzzle of autism.

Jakes family raised money for the early research, which scientists then parlayed into a $4 million grant from the National Institutes of Health to delve more deeply into Jakes gene, one of more than 100 implicated in autism. They hope to find points of convergence that could someday help people with all forms of the neurodevelopmental condition affecting one in 44 U.S. children.

Jake knows he inspired their work. And thats helped him see autism as something to be proud of rather than something that makes him different from other kids. His parents, Joe and Lisa Litvag, figured meeting the scientists and the mice would show him firsthand what he had brought into being.

Oh wow. Cool! Jake said as he watched a mouse climb down a pole while others scampered in a bin.

Walking out of the lab, tears welled up in Lisa Litvags eyes as she thought about the language within her sons cells helping other kids.

Were deeply proud and humbled to be part of this, said Joe Litvag. What do we live this life for? Its ultimately to try to, in one way, shape or form, be of service to others.

JAKES GIFT

The Litvags realized early on that Jake wasnt reaching childhood milestones. He couldnt walk without assistance until he was 4. He struggled to string sentences together in first grade.

At first, no one could pin down why. Jake had a mix of different traits. He was hyperactive and impulsive but also social, warm and funny. It took until he was 5 to get a firm diagnosis of autism.

Around that time, the Litvags heard that child psychiatrist Dr. John Constantino, an expert on the genetic underpinnings of autism, was giving a talk at the Saint Louis Science Center. They decided to go in the hopes of meeting him. They did, and he began seeing Jake as a patient.

About five years later, Constantino proposed genetic testing. It revealed the missing copy of the MYT1L gene believed to cause one out of every 10,000 to 50,000 autism cases. Having an extra copy can cause schizophrenia.

The finding brought the family peace. Theyd heard lots of people say autism was mostly caused by external factors, like birth trauma. For a long time, Lisa Litvag said, I thought it was something that I did.

Actually, a large multinational study suggests that up to 80% of the risk for autism can be traced to inherited genes.

One of the big things it did for us as a family is it made us realize that its nothing that we did wrong, Joe Litvag said. Its just that people are born all the time with genetic differences.

The couple, whose younger son Jordan doesnt have the condition, talked openly with Jake about his autism and tried to bolster his self-esteem when he worried about being seen as different. They sent him to a small private school that tailors its curriculum to each childs learning abilities. And they encouraged his social tendencies, cheering him on when he and some classmates formed a band, the Snakes.

We never wanted him to feel there was shame around his diagnosis, Lisa Litvag said. We continued to kind of reinforce that this is a superpower, you are special, you are awesome and because you have autism, there are gifts you have to give other people.

GIFTS BLOSSOM

When Constantino suggested studying the little-understood MYT1L gene, the Litvags enthusiastically agreed to help. Constantino who is on the local board of a group theyve long been active in called Autism Speaks asked if theyd be interested in raising money for early research.

Joe Litvag, an executive in the live music industry, and Lisa Litvag, a partner in a marketing firm, reached out to family and friends and raised the $70,000 needed in about six months.

With half the money, researcher Kristen Kroll and her team reprogrammed cells from Jakes blood into induced pluripotent stem cells, which can be prodded into becoming various cell types. With the other half, scientist Joseph Dougherty and his team followed the blueprint of Jakes genome and induced his mutation in mice using the gene-editing tool CRISPR.

Like the people theyre meant to model, mice with the mutation tended to be more hyperactive than siblings without it, running around their cages much more. They were nonetheless generally heavier, especially the first generation of mice. They had slightly smaller brains and a little less of the white matter that speeds communication between different brain regions.

Since starting the research about three years ago, scientists have bred around 100 mice with Jakes mutation and are now using the great-great grandchildren of the first one they engineered. They recently published about the mice in the journal Neuron.

While scientists cant go back and see how Jakes brain developed, Dougherty said, mice allow them to watch the mutation play out through generations.

A GIFT IN RETURN

Dougherty and his colleagues hope what they learn about how MYT1L functions ultimately leads to medicines or gene therapies that improve or even correct the problems the mutation causes.

They are sharing their findings with scientists studying other autism-causing genes or trying to figure out how various genes work together to cause the condition. According to the Simons Foundation Autism Research Initiative, more than 100 genes have strong evidence linking them to autism and a growing list contains several hundred more genes thought to be linked to the condition.

In cases where autism is caused by a single gene, Dougherty said that gene probably does many things to brain development. A key to understanding autism overall is to find one or two things shared across different forms of autism - which could then be targets for treatment. Though not everyone with autism wants treatment, Dougherty said it could help those who do.

Since the research began, Dougherty has been writing notes to the Litvags explaining the latest discoveries. But as a lab scientist, hes mostly removed from the people sparking the research and first met the family when they were invited by the school to visit in December.

After meeting the mice, they stopped into another lab, where Jake peered through a microscope at his blue-stained stem cells.

Thats me! Thats cool stuff. I never saw anything like that in my life, he said, stepping back to lean into his dad, who pulled him close.

Dougherty used the visit as an opportunity to share some news, a gift of sorts that he wanted to tell the family in person.

The missing gene doesnt seem to shorten life. The mice live 2 to 3 years, the same as their siblings.

So, a normal life span? Joe Litvag asked hopefully.

Yes, Dougherty answered. As far as we can tell, identical. I know thats a big relief, too.

Joe Litvag turned to his son. So Jake, maybe you will live to be 100.

I will be 112! Jake replied with a grin.

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Jake's mice: Searching for answers to the puzzle of autism - huntingdondailynews.com

Realizing the vision of culturing organs for use in life-saving transplantation procedures is still a long way off. However, the work of Prof. Jacob Hanna on stem cells is paving the way for this to become a reality.

Hanna and his team from the Weizmann Institute of Sciences Molecular Genetics Department have found a way to culture human stem cells in a much earlier state than was previously possible. Not only that, the stem cells they created are far more competent, meaning that they are able to integrate more efficiently with their host environment. This substantially improves the chances of obtaining what is called a cross-species chimera allowing cells from one creature to play a substantial role in the development of another.

The recently published findings demonstrate that very early human cells can be created and then successfully integrated into mice, owing to their undifferentiated, or nave], state, wherein they can develop into any type of cell in the body, including other stem cells. Additionally, the researchers lay out a protocol for significantly increasing the efficiency (or competence) with which these cells can integrate. Improving our ability to create and study these cell types could be used in the future to transfer cells if not organs from one animal to another, humans included.

Hannas lab broke ground in 2013 when they were the first to inject human stem cells into mice and show that they can successfully integrate into the latters developing embryos. Eight years after this study was first published, Hanna and his team felt that they could go one step further by attempting to produce an even earlier, fully naive, form of stem cells for use in similar procedures. As they were mulling over the idea, Hanna knew that this might be nearly, if not altogether, impossible to achieve. Our experience with producing similar cells in mice has taught us to expect challenging obstacles along the way, says Hanna.

These cells normally suffer from genetic, as well as epigenetic, instability, and in the end they dont differentiate too well, which is key to proper embryonic development and a prerequisite for their integration into another animals embryo. In fact, only about 1-3 percent of cells that have been transferred between species actually manage to integrate and contribute to development.

To boost these numbers, the researchers in the new study inhibited two additional signaling pathways to produce naive human stem cells having a stable genome, relatively few gene regulation glitches, and most importantly, they differentiate perfectly. The researchers also mutated an important gene that contributes to genome stability, which resulted in not only competent, but also competitive, stem cells that can integrate well without causing damage to the host. We found a way to make human stem cells more competent, and competitive, increasing the chances for a successful transfer by about fivefold compared to what we were able to do in the past, concludes Hanna.

While the previous study showed that human naive stem cells can differentiate into primordial germ cells the progenitors of egg or sperm cells the fully naive stem cells produced in the present study can also differentiate into extraembryonic tissues, the placenta and yolk sac cells that sustain the developing embryo. Such cells could be used, for example, as the source for developing synthetic embryos without the need for donor eggs. Reaching this state with mouse stem cells is particularly difficult to accomplish, Hanna explains, noting that human cells are apparently different.

This is perhaps the most surprising finding that the researchers made highlighting the differences between the behavior of human and mouse stem cells, and between the different states of naive cells. These differences expose the work that still needs to be done in making the dream of developing made-to-order organs a real-world actuality.

According to Hanna, understanding these differences will be pivotal for overcoming a myriad of issues still facing the field of stem cell research and application: If in the future we should wish to grow a pancreas in pigs for human transplantation, for example, we will have to take into account these massive evolutionary differences between species, beginning with mice and humans. For now, it would seem that Hanna and his team have taken a constructive leap in that direction.

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As Nave as They Get - Weizmann Wonder Wander - News, Features and Discoveries - Weizmann Institute of Science

Primary Cells, Stem Cells, Culture Media and Reagents Market report focused on the comprehensive analysis of current and future prospects of the Primary Cells, Stem Cells, Culture Media and Reagents industry. This report is a consolidation of primary and secondary research, which provides market size, share, dynamics, and forecast for various segments and sub-segments considering the macro and micro environmental factors. An in-depth analysis of past trends, future trends, demographics, technological advancements, and regulatory requirements for the Primary Cells, Stem Cells, Culture Media and Reagents market has been done in order to calculate the growth rates for each segment and sub-segments.

Primary cells are cells isolated from human or animal tissues. These cells are ideal models for cancer biomarker discovery and drug screening. They are also used to understand the biochemical pathways of cellular interactions.

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Top Key Vendors of this Market are:

BrainStorm Cell Therapeutics Inc., Caladrius Biosciences, Inc., Lonza, BioTime Inc., Cytori Therapeutics, Inc., ZenBio, Merck KGaA, Axol Biosciences, STEMCELL Technologies Inc., Cellular Engineering Technologies Inc., Thermo Fisher Scientific, STEMCELL Technologies, Cell Biologics, PromoCell, Takara Bio Inc., AllCells, Astellas Pharma Inc., Osiris Therapeutics, Inc..

Global Primary Cells, Stem Cells, Culture Media and Reagents Market Segmentation:

Market Segmentation: By Type

Primary Cells, Stem Cells, Culture Media, Reagents

Market Segmentation: By Application

Life Science Research Companies, Pharmaceutical Companies, Research Institutes, Others

Various factors are responsible for the markets growth trajectory, which are studied at length in the report. In addition, the report lists down the restraints that are posing threat to the global Primary Cells, Stem Cells, Culture Media and Reagents market. This report is a consolidation of primary and secondary research, which provides market size, share, dynamics, and forecast for various segments and sub-segments considering the macro and micro environmental factors. It also gauges the bargaining power of suppliers and buyers, threat from new entrants and product substitute, and the degree of competition prevailing in the market.

The report provides insights on the following pointers:

Market Penetration: Comprehensive information on the product portfolios of the top players in the Primary Cells, Stem Cells, Culture Media and Reagents market.

Competitive Assessment: In-depth assessment of the market strategies, geographic and business segments of the leading players in the market.

Product Development/Innovation: Detailed insights on the upcoming technologies, R&D activities, and product launches in the market.

Market Development: Comprehensive information about emerging markets. This report analyzes the market for various segments across geographies.

Market Diversification: Exhaustive information about new products, untapped geographies, recent developments, and investments in the Primary Cells, Stem Cells, Culture Media and Reagents market.

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The influence of the latest government guidelines is also analysed in detail in the report. It studies the Primary Cells, Stem Cells, Culture Media and Reagents markets trajectory between forecast periods. The cost analysis of the Global Primary Cells, Stem Cells, Culture Media and Reagents Market has been performed while keeping in view manufacturing expenses, labour cost, and raw materials and their market concentration rate, suppliers, and price trend.

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Table of Contents

Global Primary Cells, Stem Cells, Culture Media and Reagents Market Research Report 2022 2029

Chapter 1 Primary Cells, Stem Cells, Culture Media and Reagents Market Overview

Chapter 2 Global Economic Impact on Industry

Chapter 3 Global Market Competition by Manufacturers

Chapter 4 Global Production, Revenue (Value) by Region

Chapter 5 Global Supply (Production), Consumption, Export, Import by Regions

Chapter 6 Global Production, Revenue (Value), Price Trend by Type

Chapter 7 Global Market Analysis by Application

Chapter 8 Manufacturing Cost Analysis

Chapter 9 Industrial Chain, Sourcing Strategy and Downstream Buyers

Chapter 10 Marketing Strategy Analysis, Distributors/Traders

Chapter 11 Market Effect Factors Analysis

Chapter 12 Global Primary Cells, Stem Cells, Culture Media and Reagents Market Forecast

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Primary Cells, Stem Cells, Culture Media and Reagents Market to Witness Rapid Growth by 2029 | BrainStorm Cell Therapeutics Inc., Caladrius...

Global Gait Rehabilitation System Market: Overview

Products in the global gait rehabilitation system market, both computer aided as well as mechanical products, are used to assist people suffering from walking disabilities or difficulties in regaining their functional gait. Increase in various kinds of walking difficulties have exponentially increased the demand for the products in the global gait rehabilitation system market. Products in the global gait rehabilitation system market are now considered to be an integral part of the rehabilitation therapy plan for people suffering from movement disorders of the lower extremities. These products help patients in considerably improving their quality of life emotionally, socially, and physically.

Essentially, products and services in the global gait rehabilitation system market aid patient in functioning normally. At the same time, the products in the global gait rehabilitation system market also aid in improving the overall function of the patients body and thereby improving their quality of life. Most of the systems in the global gait rehabilitation system market are designed to treat patients suffering from subacute and acute neurological disorders. Several techniques applied by the players in the global gait rehabilitation system market include non-functional training or gait movements and gait cycle training, also known as functional gait training.

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Global Gait Rehabilitation System Market: Major Trends and Drivers

Some of the key drivers for the global gait rehabilitation system market include rising incidence of various neurological disorders as well as rising number of sports injuries. Severe sports injuries can damage and injure the ligaments and cartilage of the athletes. This can result in the requirement of gait adjustment therapies for them. This is creating new avenues in the global gait rehabilitation system market. Moreover, increase in the instances of orthopaedic disorders is also expected to fuel the demand in the global gait rehabilitation system market in coming years.

On the other hand, dearth of skilled and trained professionals as well as availability of alternative therapies may hinder the growth in the global gait rehabilitation system market during the forecast period. However, rising prevalence of orthopaedic disorders such as osteoarthritis as well as the increasing geriatric population are also driving the demand in the global gait rehabilitation system market in coming years. Rising instances of degenerative joint disease in the United States are also fuelling the demand in the global gait rehabilitation system market.

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Global Gait Rehabilitation System Market: Key Players and Manufacturers

Players and manufacturers in the global gait rehabilitation system market are adopting a number of different strategies in order to amass a larger share in the industry in coming years. Some of the leading strategies adopted by the vendors in the global gait rehabilitation system market include new product launches, strategic collaborations, expansion of product portfolios, mergers and acquisitions, and product innovations. Some of the leading and key players functional within the global gait rehabilitation system market include Zebris Medical GmbH, Reha Technology AG, Meditouch, BAMA Teknoloji, Meden Inmed Sp Z o. o., PRODROMUS S. A., DIH Medical, HIWIN Corporation, Restorative Therapies, Inc., and Groupe WINNCARE, among others.

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Global Gait Rehabilitation System Market: Regional Assessment

Key regions functional within the global gait rehabilitation system market include Europe, Middle East and Africa, Asia Pacific, South America, and North America. Regionally, North America led the global gait rehabilitation system market in recent past. Some of the key drivers for the global gait rehabilitation system market in North America include rising burden of stroke among the elderly and aging population, increase in the research and development activities as well as investments to develop new products, and rising number of new product launches in the region. The United States holds the leading share in the global gait rehabilitation system market. Furthermore, rapid adoption of products featuring new technology is also expected to drive the global gait rehabilitation system market in North America.

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Cell Culture Media, Sera, and Reagents Market: According to Transparency Market Researchs latest report on the global cell culture media, sera, and reagents market for the historical period 20172018 and forecast period 20192027, contract research and manufacturing, and focus on stem cell research would expand the scope of cell culture,growing demand for biopharmaceuticals and artificial organs, and trend towards serum-free, specialty and customized media solutions are projected to drive the global cellculture media, sera, and reagents market during the forecast period

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Newswise Rockville, Md.The Association for Research in Vision and Ophthalmology (ARVO) congratulates Magdalena Renner, MD, recipient of the 2022 Bert M. Glaser, MD Award for Innovative Research in Retina. The award recognizes an early-career investigator who has made a novel discovery that impacted the understanding and/or treatment of a retinal disease or condition.

Renner leads the Human Organoid Platform at the Institute of Molecular and Clinical Ophthalmology Basel (IOB), Basel, Switzerland. Together with her team she is optimizing the culture of retinal organoids as a model for the human retina and retinal disease. She is particularly interested in enabling the mass production of retinal organoids and other cell types of the human eye to facilitate high-content compound screening and testing of novel gene therapy tools.

Renner is also the recipient of the Swiss OphthAWARD Best Experimental Work (2021), the PRO RETINA award of RETINA Suisse and PRO RETINA Germany (2021) and won the 1st place of the VSY Biotechnology Ophthalmology Star Award (2021).

Recipients of the Bert M. Glaser, MD Award must demonstrate their work has led to the development of a new drug or technique that will revolutionize retina care. Renner and her team have developed a method to generate thousands of artificial human retinas (retinal organoids) from human pluripotent stem cells. "We compared retinal organoids to post-mortem human retina and analyzed their cell type-diversity, morphology, light-responses and gene-expression," says Renner. "Importantly, we analyzed which cell types express known disease genes in both human retina and retinal organoids which validates retinal organoids as a promising new model to study retinal disease."

Funded by the Glaser family through the ARVO Foundation, the $10,000 award is named in memory of Bert M. Glaser, MD, a long-time retina researcher, surgeon, innovator and teacher whose life was cut short in 2017 after a brief illness.Read more about Bert M. Glaser.

I am very proud and honored to receive this award in honor of Bert M. Glaser, says Renner. Being selected is a great motivation to keep on innovating and making retinal organoids an even better model system for the human retina. I sincerely hope that by using a human model system to study retinal degeneration and develop new treatments, these treatments can be translated efficiently into therapies that help patients with visual diseases.

For more information about the Bert M. Glaser, MD Award for Innovative Research in Retina, visitARVOs website.

# # #

TheAssociation for Research in Vision and Ophthalmology (ARVO)is the largest eye and vision research organization in the world. Members include approximately 10,000 eye and vision researchers from over 75 countries. ARVO advances research worldwide into understanding the visual system and preventing, treating and curing its disorders. Learn more atARVO.org.

Established in 2001, theARVO Foundation for Eye Researchraises funds through partnerships, grants and sponsorships to support ARVOs world-class education and career development resources for eye and vision researchers of all stages of career and education. Learn more atARVOFoundation.org.

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ARVO Foundation Announces 2022 Bert M. Glaser, MD Award for Innovative Retina Research Recipient - Newswise