Spontaneous contraction of cardiomyocytes differentiated using Option A (GiSB method)
19-9-11 inducible beta;-catenin shRNA cells were treated with 12 mu;M CHIR99021 at day 0 and 2 mu;g/ml dox at 36 hr. Movie S1 shows day 15 cardiomyocytes. Full protocol described in: Xiaojun Lian et al. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/ beta;-catenin signaling under fully defined conditions Nature Protocols 8, 162--175 (2013) doi:10.1038/nprot.2012.150 dx.doi.org
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Spontaneous contraction of cardiomyocytes differentiated using Option A (GiSB method) - Video
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