Recent Findings from M. Nishikawa and Co-Researchers Yields New Information on Stem Cells

By a News Reporter-Staff News Editor at Veterinary Week -- Research findings on Stem Cell Research are discussed in a new report. According to news originating from North Hills, California, by VerticalNews correspondents, research stated, "Successful derivations of specific neuronal and glial cells from embryonic stem cells have enormous potential for cell therapies and regenerative medicine. However, the low efficiency, the complexity of induction method, and the need for purification represent obstacles that make their application impractical."

Our news journalists obtained a quote from the research, "In this study, we found that PDGFR alpha(+) cells derived from mouse embryonic stem cells (mESC) can serve as a useful source from which to induce cells that express gamma-aminobutyric-acid (GABA)-releasing (GABAergic) neuronal markers. PDGFR alpha(+) cells were induced from mESC on collagen IV-coated plates in mesenchymal stem cell (MSC) culture medium with limited exposure to retinoic acid, sorted by fluorescence-activated cell sorter and maintained in MSC culture medium containing Y-27632, a Rho-associated kinase inhibitor. We found that supplementation of vascular endothelial growth factor, fibroblast growth factor-basic, and sodium azide (NaN3) to MSC culture medium effectively differentiated PDGFR alpha(+) cells into cells that express GABAergic neuronal markers, such as Pax2, Dlx2, GAD67 NCAM, and tubulin-beta III, while markers for oligodendrocyte (Sox2) and astrocyte (Glast) were suppressed. Immunostaining for GABA showed the majority (86 +/- 5%) of the induced cells were GABA-positive. We also found that the PDGFR alpha(+) cells retained such differentiation potential even after more than ten passages and cryopreservation. In summary, this study presents a simple and highly efficient method of inducing cells that express GABAergic neuronal markers from mESC."

According to the news editors, the research concluded: "Together with its ease of maintenance in vitro, PDGFR alpha(+) cells derived from mESC may serve as a useful source for such purpose."

For more information on this research see: Effective induction of cells expressing GABAergic neuronal markers from mouse embryonic stem cell. In Vitro Cellular & Developmental Biology-Animal, 2013;49(7):479-485. In Vitro Cellular & Developmental Biology-Animal can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA.

The news correspondents report that additional information may be obtained from M. Nishikawa, VAGLAHS Sepulveda, Renal Regenerat Lab, North Hills, CA 91343, United States. Additional authors for this research include N. Yanagawa, S. Yuri, P. Hauser, O.D. Jo and N. Yanagawa.

Keywords for this news article include: Biomedical Engineering, Biomedicine, Neurons, California, North Hills, United States, Bioengineering, Stem Cell Research, Embryonic Stem Cells, Regenerative Medicine, North and Central America

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Recent Findings from M. Nishikawa and Co-Researchers Yields New Information on Stem Cells